Insights on the Phytochemical Profile (Cyclopeptides) and Biological Activities of Calotropis procera Latex Organic Fractions
Calotropis procera is a medicinal plant whose pharmacological properties are associated with its latex. Here, the Calotropis procera latex fractions were investigated in an attempt to trace its phytochemical profile and measure its anti-inflammatory and toxicity activity. The crude latex was partiti...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/615454 |
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| author | Thiago Lustosa Jucá Márcio Viana Ramos Frederico Bruno Mendes Batista Moreno Mayara Patrícia Viana de Matos José Delano Barreto Marinho-Filho Renato Azevedo Moreira Ana Cristina de Oliveira Monteiro-Moreira |
| author_facet | Thiago Lustosa Jucá Márcio Viana Ramos Frederico Bruno Mendes Batista Moreno Mayara Patrícia Viana de Matos José Delano Barreto Marinho-Filho Renato Azevedo Moreira Ana Cristina de Oliveira Monteiro-Moreira |
| author_sort | Thiago Lustosa Jucá |
| collection | DOAJ |
| description | Calotropis procera is a medicinal plant whose pharmacological properties are associated with its latex. Here, the Calotropis procera latex fractions were investigated in an attempt to trace its phytochemical profile and measure its anti-inflammatory and toxicity activity. The crude latex was partitioned, yielding five fractions (49.4% hexane, 5.2% dichloromethane, 2.0% ethyl acetate, 2.1% n-butanol, and 41.1% aqueous). Phytochemical screening and spectroscopy analysis revealed that dichloromethane is the most chemically diverse fraction. Triterpenes were detected in both the hexane and dichloromethane fractions, while flavonoids were detected in the dichloromethane and ethyl acetate fractions. These fractions were cytotoxic to cancer cell lines (LD50 0.05 to 3.9 μg/mL) and lethal to brine shrimp (LD50 10.9 to 65.7 μg/mL). Reduced neutrophil migration in rats was observed in carrageenan-induced peritonitis for the dichloromethane (67%), ethyl acetate (56%), and aqueous (72%) fractions. A positive reaction with tolidine and ninhydrin suggested that cyclopeptides are in the ethyl acetate fraction. It is therefore concluded that Calotropis procera latex dichloromethane and ethyl acetate fractions exhibit both in vitro and in vivo activities as well as anti-inflammatory properties. Cyclopeptide detection is especially interesting because previous attempts to investigate these low-molecular cyclic amino acid sequences in C. procera have failed. |
| format | Article |
| id | doaj-art-085b140bca9e459aa99e81ff47bde56d |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-085b140bca9e459aa99e81ff47bde56d2025-08-20T03:55:32ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/615454615454Insights on the Phytochemical Profile (Cyclopeptides) and Biological Activities of Calotropis procera Latex Organic FractionsThiago Lustosa Jucá0Márcio Viana Ramos1Frederico Bruno Mendes Batista Moreno2Mayara Patrícia Viana de Matos3José Delano Barreto Marinho-Filho4Renato Azevedo Moreira5Ana Cristina de Oliveira Monteiro-Moreira6Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Av. Humberto Monte, Campus do Pici, 60451-970 Fortaleza, CE, BrazilDepartamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Av. Humberto Monte, Campus do Pici, 60451-970 Fortaleza, CE, BrazilDepartamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Av. Humberto Monte, Campus do Pici, 60451-970 Fortaleza, CE, BrazilDepartamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Av. Humberto Monte, Campus do Pici, 60451-970 Fortaleza, CE, BrazilDepartamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Avenue Coronel Nunes de Melo, 1127, Rodolfo Teófilo, 60430-270 Fortaleza, CE, BrazilCurso de Farmácia, Universidade de Fortaleza, Avenue Washington Soares, 1321, Bloco F, Edson Queiroz, 60811-905 Fortaleza, CE, BrazilCurso de Farmácia, Universidade de Fortaleza, Avenue Washington Soares, 1321, Bloco F, Edson Queiroz, 60811-905 Fortaleza, CE, BrazilCalotropis procera is a medicinal plant whose pharmacological properties are associated with its latex. Here, the Calotropis procera latex fractions were investigated in an attempt to trace its phytochemical profile and measure its anti-inflammatory and toxicity activity. The crude latex was partitioned, yielding five fractions (49.4% hexane, 5.2% dichloromethane, 2.0% ethyl acetate, 2.1% n-butanol, and 41.1% aqueous). Phytochemical screening and spectroscopy analysis revealed that dichloromethane is the most chemically diverse fraction. Triterpenes were detected in both the hexane and dichloromethane fractions, while flavonoids were detected in the dichloromethane and ethyl acetate fractions. These fractions were cytotoxic to cancer cell lines (LD50 0.05 to 3.9 μg/mL) and lethal to brine shrimp (LD50 10.9 to 65.7 μg/mL). Reduced neutrophil migration in rats was observed in carrageenan-induced peritonitis for the dichloromethane (67%), ethyl acetate (56%), and aqueous (72%) fractions. A positive reaction with tolidine and ninhydrin suggested that cyclopeptides are in the ethyl acetate fraction. It is therefore concluded that Calotropis procera latex dichloromethane and ethyl acetate fractions exhibit both in vitro and in vivo activities as well as anti-inflammatory properties. Cyclopeptide detection is especially interesting because previous attempts to investigate these low-molecular cyclic amino acid sequences in C. procera have failed.http://dx.doi.org/10.1155/2013/615454 |
| spellingShingle | Thiago Lustosa Jucá Márcio Viana Ramos Frederico Bruno Mendes Batista Moreno Mayara Patrícia Viana de Matos José Delano Barreto Marinho-Filho Renato Azevedo Moreira Ana Cristina de Oliveira Monteiro-Moreira Insights on the Phytochemical Profile (Cyclopeptides) and Biological Activities of Calotropis procera Latex Organic Fractions The Scientific World Journal |
| title | Insights on the Phytochemical Profile (Cyclopeptides) and Biological Activities of Calotropis procera Latex Organic Fractions |
| title_full | Insights on the Phytochemical Profile (Cyclopeptides) and Biological Activities of Calotropis procera Latex Organic Fractions |
| title_fullStr | Insights on the Phytochemical Profile (Cyclopeptides) and Biological Activities of Calotropis procera Latex Organic Fractions |
| title_full_unstemmed | Insights on the Phytochemical Profile (Cyclopeptides) and Biological Activities of Calotropis procera Latex Organic Fractions |
| title_short | Insights on the Phytochemical Profile (Cyclopeptides) and Biological Activities of Calotropis procera Latex Organic Fractions |
| title_sort | insights on the phytochemical profile cyclopeptides and biological activities of calotropis procera latex organic fractions |
| url | http://dx.doi.org/10.1155/2013/615454 |
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