Integrating Transcriptomic and Proteomic Data: IL‐27B as a Key Protein in the Development of Septic Cardiomyopathy—A Retrospective Study
ABSTRACT Background Septic cardiomyopathy (SCM) is a potentially fatal complication of sepsis. In this study, transcriptomic and proteomic analyzes of serum samples from sepsis patients were conducted to uncover the underlying pathological mechanisms and identify potential therapeutic targets for SC...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-05-01
|
| Series: | Immunity, Inflammation and Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/iid3.70207 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850122986850353152 |
|---|---|
| author | Yifeng Mao Qingqing Chen Yongpo Jiang Xijiang Zhang Qin Si Panpan Xu Zhongheng Zhang Cheng Zheng Ronghai Lin |
| author_facet | Yifeng Mao Qingqing Chen Yongpo Jiang Xijiang Zhang Qin Si Panpan Xu Zhongheng Zhang Cheng Zheng Ronghai Lin |
| author_sort | Yifeng Mao |
| collection | DOAJ |
| description | ABSTRACT Background Septic cardiomyopathy (SCM) is a potentially fatal complication of sepsis. In this study, transcriptomic and proteomic analyzes of serum samples from sepsis patients were conducted to uncover the underlying pathological mechanisms and identify potential therapeutic targets for SCM. Methods This retrospective, dual‐center study investigated the progression of sepsis to SCM in patients admitted to intensive care units. A total of 50 patients were enrolled and divided into two groups: sepsis with cardiomyopathy (25 cases) and sepsis without cardiomyopathy (25 cases). Co‐expression network analysis was employed to elucidate the biological significance of differentially expressed proteins. By integrating proteomic and transcriptomic data, molecular networks were constructed to visualize interactions among key molecules, aiming to enhance data interpretation and support the study's findings. Results Proteomic analysis identified 216 differentially expressed proteins (Fold change > 1.5, p‐value < 0.05) between the two groups. Transcriptomic analysis revealed two proteins, including Interleukin‐27 subunit beta (IL‐27B) and carbonic anhydrase, co‐downregulated in patients with septic cardiomyopathy. IL‐27B was associated with the immune response, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated its involvement in the cytokine‐cytokine receptor interaction signaling pathway. Conclusion Comprehensive integrated transcriptomic and proteomic analyzes identified significant changes in protein expression associated with SCM, primarily associated with inflammation‐related pathways and amino acid metabolism. These findings provide new insights into the pathological mechanisms of SCM and highlight potential therapeutic targets for its treatment. Trial Registration The Clinical Research Ethics Committee of Taizhou Hospital of Zhejiang Province affiliated to Wenzhou Medical University approved this study, and written informed consent was given by all patients or their legal representatives. (NO.K20201110). |
| format | Article |
| id | doaj-art-08595433c57f4e6da906ba755d038e3d |
| institution | OA Journals |
| issn | 2050-4527 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj-art-08595433c57f4e6da906ba755d038e3d2025-08-20T02:34:42ZengWileyImmunity, Inflammation and Disease2050-45272025-05-01135n/an/a10.1002/iid3.70207Integrating Transcriptomic and Proteomic Data: IL‐27B as a Key Protein in the Development of Septic Cardiomyopathy—A Retrospective StudyYifeng Mao0Qingqing Chen1Yongpo Jiang2Xijiang Zhang3Qin Si4Panpan Xu5Zhongheng Zhang6Cheng Zheng7Ronghai Lin8Department of Critical Care Medicine Municipal Hospital Affiliated to Taizhou University Taizhou ZheJiang Province ChinaDepartment of Critical Care Medicine Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University Linhai Zhejiang Province ChinaDepartment of Critical Care Medicine Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University Linhai Zhejiang Province ChinaDepartment of Critical Care Medicine Municipal Hospital Affiliated to Taizhou University Taizhou ZheJiang Province ChinaDepartment of Critical Care Medicine Municipal Hospital Affiliated to Taizhou University Taizhou ZheJiang Province ChinaDepartment of Critical Care Medicine Municipal Hospital Affiliated to Taizhou University Taizhou ZheJiang Province ChinaDepartment of Emergency Medicine, Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou Zhejiang Province ChinaDepartment of Critical Care Medicine Municipal Hospital Affiliated to Taizhou University Taizhou ZheJiang Province ChinaDepartment of Critical Care Medicine Municipal Hospital Affiliated to Taizhou University Taizhou ZheJiang Province ChinaABSTRACT Background Septic cardiomyopathy (SCM) is a potentially fatal complication of sepsis. In this study, transcriptomic and proteomic analyzes of serum samples from sepsis patients were conducted to uncover the underlying pathological mechanisms and identify potential therapeutic targets for SCM. Methods This retrospective, dual‐center study investigated the progression of sepsis to SCM in patients admitted to intensive care units. A total of 50 patients were enrolled and divided into two groups: sepsis with cardiomyopathy (25 cases) and sepsis without cardiomyopathy (25 cases). Co‐expression network analysis was employed to elucidate the biological significance of differentially expressed proteins. By integrating proteomic and transcriptomic data, molecular networks were constructed to visualize interactions among key molecules, aiming to enhance data interpretation and support the study's findings. Results Proteomic analysis identified 216 differentially expressed proteins (Fold change > 1.5, p‐value < 0.05) between the two groups. Transcriptomic analysis revealed two proteins, including Interleukin‐27 subunit beta (IL‐27B) and carbonic anhydrase, co‐downregulated in patients with septic cardiomyopathy. IL‐27B was associated with the immune response, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated its involvement in the cytokine‐cytokine receptor interaction signaling pathway. Conclusion Comprehensive integrated transcriptomic and proteomic analyzes identified significant changes in protein expression associated with SCM, primarily associated with inflammation‐related pathways and amino acid metabolism. These findings provide new insights into the pathological mechanisms of SCM and highlight potential therapeutic targets for its treatment. Trial Registration The Clinical Research Ethics Committee of Taizhou Hospital of Zhejiang Province affiliated to Wenzhou Medical University approved this study, and written informed consent was given by all patients or their legal representatives. (NO.K20201110).https://doi.org/10.1002/iid3.70207multiomicsproteomicsseptic cardiomyopathytranscriptomics |
| spellingShingle | Yifeng Mao Qingqing Chen Yongpo Jiang Xijiang Zhang Qin Si Panpan Xu Zhongheng Zhang Cheng Zheng Ronghai Lin Integrating Transcriptomic and Proteomic Data: IL‐27B as a Key Protein in the Development of Septic Cardiomyopathy—A Retrospective Study Immunity, Inflammation and Disease multiomics proteomics septic cardiomyopathy transcriptomics |
| title | Integrating Transcriptomic and Proteomic Data: IL‐27B as a Key Protein in the Development of Septic Cardiomyopathy—A Retrospective Study |
| title_full | Integrating Transcriptomic and Proteomic Data: IL‐27B as a Key Protein in the Development of Septic Cardiomyopathy—A Retrospective Study |
| title_fullStr | Integrating Transcriptomic and Proteomic Data: IL‐27B as a Key Protein in the Development of Septic Cardiomyopathy—A Retrospective Study |
| title_full_unstemmed | Integrating Transcriptomic and Proteomic Data: IL‐27B as a Key Protein in the Development of Septic Cardiomyopathy—A Retrospective Study |
| title_short | Integrating Transcriptomic and Proteomic Data: IL‐27B as a Key Protein in the Development of Septic Cardiomyopathy—A Retrospective Study |
| title_sort | integrating transcriptomic and proteomic data il 27b as a key protein in the development of septic cardiomyopathy a retrospective study |
| topic | multiomics proteomics septic cardiomyopathy transcriptomics |
| url | https://doi.org/10.1002/iid3.70207 |
| work_keys_str_mv | AT yifengmao integratingtranscriptomicandproteomicdatail27basakeyproteininthedevelopmentofsepticcardiomyopathyaretrospectivestudy AT qingqingchen integratingtranscriptomicandproteomicdatail27basakeyproteininthedevelopmentofsepticcardiomyopathyaretrospectivestudy AT yongpojiang integratingtranscriptomicandproteomicdatail27basakeyproteininthedevelopmentofsepticcardiomyopathyaretrospectivestudy AT xijiangzhang integratingtranscriptomicandproteomicdatail27basakeyproteininthedevelopmentofsepticcardiomyopathyaretrospectivestudy AT qinsi integratingtranscriptomicandproteomicdatail27basakeyproteininthedevelopmentofsepticcardiomyopathyaretrospectivestudy AT panpanxu integratingtranscriptomicandproteomicdatail27basakeyproteininthedevelopmentofsepticcardiomyopathyaretrospectivestudy AT zhonghengzhang integratingtranscriptomicandproteomicdatail27basakeyproteininthedevelopmentofsepticcardiomyopathyaretrospectivestudy AT chengzheng integratingtranscriptomicandproteomicdatail27basakeyproteininthedevelopmentofsepticcardiomyopathyaretrospectivestudy AT ronghailin integratingtranscriptomicandproteomicdatail27basakeyproteininthedevelopmentofsepticcardiomyopathyaretrospectivestudy |