A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH
Background. FISH is a molecular cytogenetic technique enabling rapid detection of genetic abnormalities. Facilities that can run fresh/wet samples for molecular diagnosis and monitoring of neoplastic disorders are not readily available in Ghana and other neighbouring countries. This study aims to de...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Advances in Hematology |
| Online Access: | http://dx.doi.org/10.1155/2014/604165 |
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| author | Alice Charwudzi Edeghonghon E. Olayemi Ivy Ekem Olufunmilayo Olopade Mariann Coyle Amma Anima Benneh Emmanuel Alote Allotey |
| author_facet | Alice Charwudzi Edeghonghon E. Olayemi Ivy Ekem Olufunmilayo Olopade Mariann Coyle Amma Anima Benneh Emmanuel Alote Allotey |
| author_sort | Alice Charwudzi |
| collection | DOAJ |
| description | Background. FISH is a molecular cytogenetic technique enabling rapid detection of genetic abnormalities. Facilities that can run fresh/wet samples for molecular diagnosis and monitoring of neoplastic disorders are not readily available in Ghana and other neighbouring countries. This study aims to demonstrate that interphase FISH can successfully be applied to archival methanol-fixed bone marrow and peripheral blood smear slides transported to a more equipped facility for molecular diagnosis of CML. Methods. Interphase FISH was performed on 22 archival methanol-fixed marrow (BM) and 3 peripheral blood (PB) smear slides obtained at diagnosis. The BM smears included 20 CML and 2 CMML cases diagnosed by morphology; the 3 PB smears were from 3 of the CML patients at the time of diagnosis. Six cases had known BCR-ABL fusion results at diagnosis by RQ-PCR. Full blood count reports at diagnosis were also retrieved. Result. 19 (95%) of the CML marrow smears demonstrated the BCR-ABL translocation. There was a significant correlation between the BCR-ABL transcript detected at diagnosis by RQ-PCR and that retrospectively detected by FISH from the aged BM smears at diagnosis (r=0.870; P=0.035). Conclusion. Archival methanol-fixed marrow and peripheral blood smears can be used to detect the BCR-ABL transcript for CML diagnosis. |
| format | Article |
| id | doaj-art-084c668dffee4d26b80834b7d626c80c |
| institution | OA Journals |
| issn | 1687-9104 1687-9112 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Hematology |
| spelling | doaj-art-084c668dffee4d26b80834b7d626c80c2025-08-20T02:01:35ZengWileyAdvances in Hematology1687-91041687-91122014-01-01201410.1155/2014/604165604165A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISHAlice Charwudzi0Edeghonghon E. Olayemi1Ivy Ekem2Olufunmilayo Olopade3Mariann Coyle4Amma Anima Benneh5Emmanuel Alote Allotey6Department of Chemical Pathology, University of Cape Coast School of Medical Sciences, Cape Coast, GhanaDepartment of Haematology, University of Ghana Medical School, Accra, GhanaDepartment of Haematology, University of Ghana Medical School, Accra, GhanaDepartment of Medicine, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USADepartment of Medicine, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USADepartment of Haematology, Korle-Bu Teaching Hospital, Accra, GhanaHaematology Unit, Tamale Teaching Hospital, Tamale, GhanaBackground. FISH is a molecular cytogenetic technique enabling rapid detection of genetic abnormalities. Facilities that can run fresh/wet samples for molecular diagnosis and monitoring of neoplastic disorders are not readily available in Ghana and other neighbouring countries. This study aims to demonstrate that interphase FISH can successfully be applied to archival methanol-fixed bone marrow and peripheral blood smear slides transported to a more equipped facility for molecular diagnosis of CML. Methods. Interphase FISH was performed on 22 archival methanol-fixed marrow (BM) and 3 peripheral blood (PB) smear slides obtained at diagnosis. The BM smears included 20 CML and 2 CMML cases diagnosed by morphology; the 3 PB smears were from 3 of the CML patients at the time of diagnosis. Six cases had known BCR-ABL fusion results at diagnosis by RQ-PCR. Full blood count reports at diagnosis were also retrieved. Result. 19 (95%) of the CML marrow smears demonstrated the BCR-ABL translocation. There was a significant correlation between the BCR-ABL transcript detected at diagnosis by RQ-PCR and that retrospectively detected by FISH from the aged BM smears at diagnosis (r=0.870; P=0.035). Conclusion. Archival methanol-fixed marrow and peripheral blood smears can be used to detect the BCR-ABL transcript for CML diagnosis.http://dx.doi.org/10.1155/2014/604165 |
| spellingShingle | Alice Charwudzi Edeghonghon E. Olayemi Ivy Ekem Olufunmilayo Olopade Mariann Coyle Amma Anima Benneh Emmanuel Alote Allotey A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH Advances in Hematology |
| title | A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH |
| title_full | A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH |
| title_fullStr | A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH |
| title_full_unstemmed | A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH |
| title_short | A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH |
| title_sort | preliminary study of the suitability of archival bone marrow and peripheral blood smears for diagnosis of cml using fish |
| url | http://dx.doi.org/10.1155/2014/604165 |
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