Sensitivity Characterization of an Impedance-Based Platform for Viability Analysis of 3D Spheroids
Electrical impedance spectroscopy (EIS) is a promising label-free tool for high-throughput analysis of 3D cellular constructs, also called spheroids. Here, we used an EIS platform featuring facing electrodes to characterize the viability of hepatic spheroids, which are used for bioprinting applicati...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-04-01
|
| Series: | Proceedings |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2504-3900/97/1/132 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850084976418095104 |
|---|---|
| author | Claudia Sampaio da Silva Christian Beyer Julia Boos Sreedhar Kumar Mario Modena Thomas Valentin Andreas Hierlemann Vincent Revol |
| author_facet | Claudia Sampaio da Silva Christian Beyer Julia Boos Sreedhar Kumar Mario Modena Thomas Valentin Andreas Hierlemann Vincent Revol |
| author_sort | Claudia Sampaio da Silva |
| collection | DOAJ |
| description | Electrical impedance spectroscopy (EIS) is a promising label-free tool for high-throughput analysis of 3D cellular constructs, also called spheroids. Here, we used an EIS platform featuring facing electrodes to characterize the viability of hepatic spheroids, which are used for bioprinting applications. By using principal component analysis (PCA), we show that this simple impedance sensor enables us to successfully distinguish healthy spheroids from spheroids exposed to toxic conditions. The sensitivity of the impedance sensor will be further characterized by using spheroids exposed to varying stress conditions like different drug concentrations and temperatures. |
| format | Article |
| id | doaj-art-084831ee5c154a42b8968cd593df9a24 |
| institution | DOAJ |
| issn | 2504-3900 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proceedings |
| spelling | doaj-art-084831ee5c154a42b8968cd593df9a242025-08-20T02:43:50ZengMDPI AGProceedings2504-39002024-04-0197113210.3390/proceedings2024097132Sensitivity Characterization of an Impedance-Based Platform for Viability Analysis of 3D SpheroidsClaudia Sampaio da Silva0Christian Beyer1Julia Boos2Sreedhar Kumar3Mario Modena4Thomas Valentin5Andreas Hierlemann6Vincent Revol7CSEM SA, 6055 Alpnach, SwitzerlandCSEM SA, 6055 Alpnach, SwitzerlandBio Engineering Laboratory, Department of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, SwitzerlandBio Engineering Laboratory, Department of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, SwitzerlandBio Engineering Laboratory, Department of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, SwitzerlandCSEM SA, 6055 Alpnach, SwitzerlandBio Engineering Laboratory, Department of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, SwitzerlandCSEM SA, 6055 Alpnach, SwitzerlandElectrical impedance spectroscopy (EIS) is a promising label-free tool for high-throughput analysis of 3D cellular constructs, also called spheroids. Here, we used an EIS platform featuring facing electrodes to characterize the viability of hepatic spheroids, which are used for bioprinting applications. By using principal component analysis (PCA), we show that this simple impedance sensor enables us to successfully distinguish healthy spheroids from spheroids exposed to toxic conditions. The sensitivity of the impedance sensor will be further characterized by using spheroids exposed to varying stress conditions like different drug concentrations and temperatures.https://www.mdpi.com/2504-3900/97/1/132impedance sensorlabel-free3D cellular constructsspectroscopybioprinting |
| spellingShingle | Claudia Sampaio da Silva Christian Beyer Julia Boos Sreedhar Kumar Mario Modena Thomas Valentin Andreas Hierlemann Vincent Revol Sensitivity Characterization of an Impedance-Based Platform for Viability Analysis of 3D Spheroids Proceedings impedance sensor label-free 3D cellular constructs spectroscopy bioprinting |
| title | Sensitivity Characterization of an Impedance-Based Platform for Viability Analysis of 3D Spheroids |
| title_full | Sensitivity Characterization of an Impedance-Based Platform for Viability Analysis of 3D Spheroids |
| title_fullStr | Sensitivity Characterization of an Impedance-Based Platform for Viability Analysis of 3D Spheroids |
| title_full_unstemmed | Sensitivity Characterization of an Impedance-Based Platform for Viability Analysis of 3D Spheroids |
| title_short | Sensitivity Characterization of an Impedance-Based Platform for Viability Analysis of 3D Spheroids |
| title_sort | sensitivity characterization of an impedance based platform for viability analysis of 3d spheroids |
| topic | impedance sensor label-free 3D cellular constructs spectroscopy bioprinting |
| url | https://www.mdpi.com/2504-3900/97/1/132 |
| work_keys_str_mv | AT claudiasampaiodasilva sensitivitycharacterizationofanimpedancebasedplatformforviabilityanalysisof3dspheroids AT christianbeyer sensitivitycharacterizationofanimpedancebasedplatformforviabilityanalysisof3dspheroids AT juliaboos sensitivitycharacterizationofanimpedancebasedplatformforviabilityanalysisof3dspheroids AT sreedharkumar sensitivitycharacterizationofanimpedancebasedplatformforviabilityanalysisof3dspheroids AT mariomodena sensitivitycharacterizationofanimpedancebasedplatformforviabilityanalysisof3dspheroids AT thomasvalentin sensitivitycharacterizationofanimpedancebasedplatformforviabilityanalysisof3dspheroids AT andreashierlemann sensitivitycharacterizationofanimpedancebasedplatformforviabilityanalysisof3dspheroids AT vincentrevol sensitivitycharacterizationofanimpedancebasedplatformforviabilityanalysisof3dspheroids |