Topical Adipose Mesenchymal Stem cell metabolites regulate the expression of MMP-1, MMP-9, EGF, TGF-β in oral mucosa ulcer rat model
Objective: Matrix metalloproteinase (MMP-1 and −9), Epidermal Growth Factor (EGF), and Transforming Growth factor (TGF)-β are expressed in the oral ulcer wound-healing process. The Adipose mesenchymal stem cell metabolites (AdMSCMs) may accelerate wound-healing. This study aimed to investigate the e...
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| Format: | Article |
| Language: | English |
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Springer
2024-06-01
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| Series: | Saudi Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905224001093 |
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| author | Hendri Susanto Ade Ratu Mas Saraswati Alexander Patera Nugraha Satutya Wicaksono Nanan Nur'aeny Diah Savitri Ernawati |
| author_facet | Hendri Susanto Ade Ratu Mas Saraswati Alexander Patera Nugraha Satutya Wicaksono Nanan Nur'aeny Diah Savitri Ernawati |
| author_sort | Hendri Susanto |
| collection | DOAJ |
| description | Objective: Matrix metalloproteinase (MMP-1 and −9), Epidermal Growth Factor (EGF), and Transforming Growth factor (TGF)-β are expressed in the oral ulcer wound-healing process. The Adipose mesenchymal stem cell metabolites (AdMSCMs) may accelerate wound-healing. This study aimed to investigate the expression of MMP-1, MMP-9, EGF, and TGF-β in the oral mucosa ulcer rat model treated with topical AdMSCMs. Materials and Methods: Oral ulcer models were created in twenty healthy male Wistar rats (Rattus novergicus) divided into AdMSCMs and control groups. The oral ulcer model was treated topically using AdMSCMs oral gel three times daily for 3 and 7 days. The expression of MMP-1, MMP-9, EGF, TGF-β was evaluated through histological examination using the immunohistochemistry method. Independent t-test was used to compare the mean of expression of MMP-1, MMP-9, EGF, TGF-β between control and treatment groups (AdMSCMs), and paired t-test was used to analyze the mean between day 3 and day 7 of each group. Results: A lower expression of MMP-1, MMP-9 in AdMSCMs group and higher expression EGF and TGF-β in AdMSCMs group compared to the control group in day 3 and day 7. Independent t-test results showed a significant difference in the expression of MMP-1, MMP-9, EGF between the control and AdMSCMs group in day 3 and day 7. Only TGF-β expression mean difference between day 3 and day 7 showed a significant difference compared to the control groups (p < 0.05) Conclusions: AdMSCMs oral gel may accelerate oral ulcer healing models by reducing the expression MMP-1, MMP-9, and increasing EGF and TGF-β expressions during the wound-healing process. |
| format | Article |
| id | doaj-art-083a62c09bdc47e9bbfedaa8e50b03bb |
| institution | DOAJ |
| issn | 1013-9052 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Springer |
| record_format | Article |
| series | Saudi Dental Journal |
| spelling | doaj-art-083a62c09bdc47e9bbfedaa8e50b03bb2025-08-20T03:17:23ZengSpringerSaudi Dental Journal1013-90522024-06-0136693293910.1016/j.sdentj.2024.03.021Topical Adipose Mesenchymal Stem cell metabolites regulate the expression of MMP-1, MMP-9, EGF, TGF-β in oral mucosa ulcer rat modelHendri Susanto0Ade Ratu Mas Saraswati1Alexander Patera Nugraha2Satutya Wicaksono3Nanan Nur'aeny4Diah Savitri Ernawati5Departement of Oral Medicine, Faculty of Dentistry, Universitas Gadjah Mada, Indonesia; Corresponding author at: Department of Oral Medicine, Faculty of Dentistry, Universitas Gadjah Mada, Denta Sekip Utara, Yogyakarta 55281, Indonesia.Clinical Dentistry, Magister Study Program, Faculty of Dentistry, Universitas Gadjah Mada, IndonesiaDepartement of Orthodontic, Faculty of Dentistry, Universitas Airlangga, IndonesiaDepartment of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, IndonesiaDepartement of Oral Medicine, Faculty of Dentistry, Universitas Padjajaran, IndonesiaDepartment of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, IndonesiaObjective: Matrix metalloproteinase (MMP-1 and −9), Epidermal Growth Factor (EGF), and Transforming Growth factor (TGF)-β are expressed in the oral ulcer wound-healing process. The Adipose mesenchymal stem cell metabolites (AdMSCMs) may accelerate wound-healing. This study aimed to investigate the expression of MMP-1, MMP-9, EGF, and TGF-β in the oral mucosa ulcer rat model treated with topical AdMSCMs. Materials and Methods: Oral ulcer models were created in twenty healthy male Wistar rats (Rattus novergicus) divided into AdMSCMs and control groups. The oral ulcer model was treated topically using AdMSCMs oral gel three times daily for 3 and 7 days. The expression of MMP-1, MMP-9, EGF, TGF-β was evaluated through histological examination using the immunohistochemistry method. Independent t-test was used to compare the mean of expression of MMP-1, MMP-9, EGF, TGF-β between control and treatment groups (AdMSCMs), and paired t-test was used to analyze the mean between day 3 and day 7 of each group. Results: A lower expression of MMP-1, MMP-9 in AdMSCMs group and higher expression EGF and TGF-β in AdMSCMs group compared to the control group in day 3 and day 7. Independent t-test results showed a significant difference in the expression of MMP-1, MMP-9, EGF between the control and AdMSCMs group in day 3 and day 7. Only TGF-β expression mean difference between day 3 and day 7 showed a significant difference compared to the control groups (p < 0.05) Conclusions: AdMSCMs oral gel may accelerate oral ulcer healing models by reducing the expression MMP-1, MMP-9, and increasing EGF and TGF-β expressions during the wound-healing process.http://www.sciencedirect.com/science/article/pii/S1013905224001093Adipose Mesenchymal Stem Cell MetabolitesMMP-1MMP-9EGFTGF-βOral mucosa Ulcer rat Model |
| spellingShingle | Hendri Susanto Ade Ratu Mas Saraswati Alexander Patera Nugraha Satutya Wicaksono Nanan Nur'aeny Diah Savitri Ernawati Topical Adipose Mesenchymal Stem cell metabolites regulate the expression of MMP-1, MMP-9, EGF, TGF-β in oral mucosa ulcer rat model Saudi Dental Journal Adipose Mesenchymal Stem Cell Metabolites MMP-1 MMP-9 EGF TGF-β Oral mucosa Ulcer rat Model |
| title | Topical Adipose Mesenchymal Stem cell metabolites regulate the expression of MMP-1, MMP-9, EGF, TGF-β in oral mucosa ulcer rat model |
| title_full | Topical Adipose Mesenchymal Stem cell metabolites regulate the expression of MMP-1, MMP-9, EGF, TGF-β in oral mucosa ulcer rat model |
| title_fullStr | Topical Adipose Mesenchymal Stem cell metabolites regulate the expression of MMP-1, MMP-9, EGF, TGF-β in oral mucosa ulcer rat model |
| title_full_unstemmed | Topical Adipose Mesenchymal Stem cell metabolites regulate the expression of MMP-1, MMP-9, EGF, TGF-β in oral mucosa ulcer rat model |
| title_short | Topical Adipose Mesenchymal Stem cell metabolites regulate the expression of MMP-1, MMP-9, EGF, TGF-β in oral mucosa ulcer rat model |
| title_sort | topical adipose mesenchymal stem cell metabolites regulate the expression of mmp 1 mmp 9 egf tgf β in oral mucosa ulcer rat model |
| topic | Adipose Mesenchymal Stem Cell Metabolites MMP-1 MMP-9 EGF TGF-β Oral mucosa Ulcer rat Model |
| url | http://www.sciencedirect.com/science/article/pii/S1013905224001093 |
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