Modern trends in identification of causative agents in infective endocarditis

Advances in the diagnosis and treatment of patients with infectious endocarditis are limited by the high frequency of cases with an unknown etiology and imperfection of microbiological (cultural) methods. To overcome these problems new approaches to the identification of infectious endocarditis path...

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Main Authors: E. O. Kotova, E. A. Domonova, Zh. D. Kobalava, J. L. Karaulova, A. S. Pisaryuk, A. V. Balatskiy, V. G. Akimkin
Format: Article
Language:English
Published: Столичная издательская компания 2021-03-01
Series:Рациональная фармакотерапия в кардиологии
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Online Access:https://www.rpcardio.online/jour/article/view/2401
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author E. O. Kotova
E. A. Domonova
Zh. D. Kobalava
J. L. Karaulova
A. S. Pisaryuk
A. V. Balatskiy
V. G. Akimkin
author_facet E. O. Kotova
E. A. Domonova
Zh. D. Kobalava
J. L. Karaulova
A. S. Pisaryuk
A. V. Balatskiy
V. G. Akimkin
author_sort E. O. Kotova
collection DOAJ
description Advances in the diagnosis and treatment of patients with infectious endocarditis are limited by the high frequency of cases with an unknown etiology and imperfection of microbiological (cultural) methods. To overcome these problems new approaches to the identification of infectious endocarditis pathogens were introduced, which allowed achieving certain positive results. However, it should be noted that despite the wide variety of diagnostic tools currently used, there is no ideal method for etiological laboratory diagnosis of infectious endocarditis. The article discusses the features and place of immunochemical, molecular biological (MALDI-TOF MS, real-time PCR, sequencing, in situ fluorescence hybridization, metagenomic methods, etc.), immunohistochemical methods, and their advantages and limitations.
format Article
id doaj-art-082c72c82efd4028ba06ead2ac464057
institution Kabale University
issn 1819-6446
2225-3653
language English
publishDate 2021-03-01
publisher Столичная издательская компания
record_format Article
series Рациональная фармакотерапия в кардиологии
spelling doaj-art-082c72c82efd4028ba06ead2ac4640572025-08-23T10:00:33ZengСтоличная издательская компанияРациональная фармакотерапия в кардиологии1819-64462225-36532021-03-0117115316410.20996/1819-6446-2021-02-141853Modern trends in identification of causative agents in infective endocarditisE. O. Kotova0E. A. Domonova1Zh. D. Kobalava2J. L. Karaulova3A. S. Pisaryuk4A. V. Balatskiy5V. G. Akimkin6Peoples’ Friendship University of Russia (RUDN)Central Research Institute of EpidemiologyPeoples’ Friendship University of Russia (RUDN)Peoples’ Friendship University of Russia (RUDN)Peoples’ Friendship University of Russia (RUDN); Moscow City Hospital named after V.V. VinogradovLomonosov Moscow State UniversityCentral Research Institute of EpidemiologyAdvances in the diagnosis and treatment of patients with infectious endocarditis are limited by the high frequency of cases with an unknown etiology and imperfection of microbiological (cultural) methods. To overcome these problems new approaches to the identification of infectious endocarditis pathogens were introduced, which allowed achieving certain positive results. However, it should be noted that despite the wide variety of diagnostic tools currently used, there is no ideal method for etiological laboratory diagnosis of infectious endocarditis. The article discusses the features and place of immunochemical, molecular biological (MALDI-TOF MS, real-time PCR, sequencing, in situ fluorescence hybridization, metagenomic methods, etc.), immunohistochemical methods, and their advantages and limitations.https://www.rpcardio.online/jour/article/view/2401culture negative infective endocarditismicrobiological methodsmaldi-tof msreal-time pcrmicrobiologistic
spellingShingle E. O. Kotova
E. A. Domonova
Zh. D. Kobalava
J. L. Karaulova
A. S. Pisaryuk
A. V. Balatskiy
V. G. Akimkin
Modern trends in identification of causative agents in infective endocarditis
Рациональная фармакотерапия в кардиологии
culture negative infective endocarditis
microbiological methods
maldi-tof ms
real-time pcr
microbiologistic
title Modern trends in identification of causative agents in infective endocarditis
title_full Modern trends in identification of causative agents in infective endocarditis
title_fullStr Modern trends in identification of causative agents in infective endocarditis
title_full_unstemmed Modern trends in identification of causative agents in infective endocarditis
title_short Modern trends in identification of causative agents in infective endocarditis
title_sort modern trends in identification of causative agents in infective endocarditis
topic culture negative infective endocarditis
microbiological methods
maldi-tof ms
real-time pcr
microbiologistic
url https://www.rpcardio.online/jour/article/view/2401
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AT jlkaraulova moderntrendsinidentificationofcausativeagentsininfectiveendocarditis
AT aspisaryuk moderntrendsinidentificationofcausativeagentsininfectiveendocarditis
AT avbalatskiy moderntrendsinidentificationofcausativeagentsininfectiveendocarditis
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