Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens
Abstract Background Avian pox viruses (APVs) are highly contagious poultry diseases, posing a major economic threat to poultry production systems, and have a high mortality rate among young birds, Infected birds also face condemnation of affected carcasses due to the unsightly appearance of the nodu...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2025-01-01
|
| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43088-024-00589-4 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832571421814947840 |
|---|---|
| author | Amira M. Helal Osama Mahana Ali Zanaty Karim Selim Mohamed. E. Taha Mostafa M. Saleh Mohamed I. Hassan Mona S. Abdallah |
| author_facet | Amira M. Helal Osama Mahana Ali Zanaty Karim Selim Mohamed. E. Taha Mostafa M. Saleh Mohamed I. Hassan Mona S. Abdallah |
| author_sort | Amira M. Helal |
| collection | DOAJ |
| description | Abstract Background Avian pox viruses (APVs) are highly contagious poultry diseases, posing a major economic threat to poultry production systems, and have a high mortality rate among young birds, Infected birds also face condemnation of affected carcasses due to the unsightly appearance of the nodular skin lesions. This study aimed to provide an overview of the current genetic status of APV in backyard poultry, with a focus on the commercially available vaccines. To achieve this, molecular characterization and phylogenetic analysis of APVs were conducted, comparing their sequences with vaccine strains used in Egypt. Results The polymerase chain reaction (PCR) assay was able to detect APV in all the tested samples; 12 positive samples (6 chicken flocks and 6 pigeon flocks) were selected for DNA sequencing. The sequences were submitted to GenBank with accession numbers OR027032 to OR027043. The chicken strains exhibited 100% nucleotide identity with commercially available fowl pox virus (FPV) vaccines and were phylogenetically clustered within subclade A1 with other FPVs. On the other hand, the pigeon pox virus (PPV) strains were closely related to other PPV strains within subclade A2 and showing 100% and 91% nucleotide identity with the PPV and the FPV vaccines, respectively. Conclusions Despite the availability of APV vaccines in Egypt, a persistent threat of APV to poultry in the backyard system remains a significant concern. Our molecular characterization revealed the high genetic similarity between our field strains and commercially available vaccine strains, suggesting an urgent need for vaccination in backyard systems to counteract this emerging threat to bird populations. |
| format | Article |
| id | doaj-art-0819f2f6f9664043a91333798ce43221 |
| institution | Kabale University |
| issn | 2314-8543 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj-art-0819f2f6f9664043a91333798ce432212025-02-02T12:36:55ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432025-01-011411810.1186/s43088-024-00589-4Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickensAmira M. Helal0Osama Mahana1Ali Zanaty2Karim Selim3Mohamed. E. Taha4Mostafa M. Saleh5Mohamed I. Hassan6Mona S. Abdallah7Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Center (ARC)Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Center (ARC)Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Center (ARC)Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Center (ARC)Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center (ARC)Animal Health Research Institute, Agricultural Research Center (ARC)Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center (ARC)Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Suez Canal UniversityAbstract Background Avian pox viruses (APVs) are highly contagious poultry diseases, posing a major economic threat to poultry production systems, and have a high mortality rate among young birds, Infected birds also face condemnation of affected carcasses due to the unsightly appearance of the nodular skin lesions. This study aimed to provide an overview of the current genetic status of APV in backyard poultry, with a focus on the commercially available vaccines. To achieve this, molecular characterization and phylogenetic analysis of APVs were conducted, comparing their sequences with vaccine strains used in Egypt. Results The polymerase chain reaction (PCR) assay was able to detect APV in all the tested samples; 12 positive samples (6 chicken flocks and 6 pigeon flocks) were selected for DNA sequencing. The sequences were submitted to GenBank with accession numbers OR027032 to OR027043. The chicken strains exhibited 100% nucleotide identity with commercially available fowl pox virus (FPV) vaccines and were phylogenetically clustered within subclade A1 with other FPVs. On the other hand, the pigeon pox virus (PPV) strains were closely related to other PPV strains within subclade A2 and showing 100% and 91% nucleotide identity with the PPV and the FPV vaccines, respectively. Conclusions Despite the availability of APV vaccines in Egypt, a persistent threat of APV to poultry in the backyard system remains a significant concern. Our molecular characterization revealed the high genetic similarity between our field strains and commercially available vaccine strains, suggesting an urgent need for vaccination in backyard systems to counteract this emerging threat to bird populations.https://doi.org/10.1186/s43088-024-00589-4MolecularPhylogeneticPoxSequencingVaccine |
| spellingShingle | Amira M. Helal Osama Mahana Ali Zanaty Karim Selim Mohamed. E. Taha Mostafa M. Saleh Mohamed I. Hassan Mona S. Abdallah Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens Beni-Suef University Journal of Basic and Applied Sciences Molecular Phylogenetic Pox Sequencing Vaccine |
| title | Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens |
| title_full | Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens |
| title_fullStr | Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens |
| title_full_unstemmed | Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens |
| title_short | Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens |
| title_sort | identification and genetic analysis of the recent avian pox viruses from infected pigeons and chickens |
| topic | Molecular Phylogenetic Pox Sequencing Vaccine |
| url | https://doi.org/10.1186/s43088-024-00589-4 |
| work_keys_str_mv | AT amiramhelal identificationandgeneticanalysisoftherecentavianpoxvirusesfrominfectedpigeonsandchickens AT osamamahana identificationandgeneticanalysisoftherecentavianpoxvirusesfrominfectedpigeonsandchickens AT alizanaty identificationandgeneticanalysisoftherecentavianpoxvirusesfrominfectedpigeonsandchickens AT karimselim identificationandgeneticanalysisoftherecentavianpoxvirusesfrominfectedpigeonsandchickens AT mohamedetaha identificationandgeneticanalysisoftherecentavianpoxvirusesfrominfectedpigeonsandchickens AT mostafamsaleh identificationandgeneticanalysisoftherecentavianpoxvirusesfrominfectedpigeonsandchickens AT mohamedihassan identificationandgeneticanalysisoftherecentavianpoxvirusesfrominfectedpigeonsandchickens AT monasabdallah identificationandgeneticanalysisoftherecentavianpoxvirusesfrominfectedpigeonsandchickens |