Branched RNA: A New Architecture for RNA Interference
Branched RNAs with two and four strands were synthesized. These structures were used to obtain branched siRNA. The branched siRNA duplexes had similar inhibitory capacity as those of unmodified siRNA duplexes, as deduced from gene silencing experiments of the TNF-α protein. Branched RNAs are conside...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.4061/2011/586935 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850238123053678592 |
|---|---|
| author | Anna Aviñó Sandra M. Ocampo José Carlos Perales Ramon Eritja |
| author_facet | Anna Aviñó Sandra M. Ocampo José Carlos Perales Ramon Eritja |
| author_sort | Anna Aviñó |
| collection | DOAJ |
| description | Branched RNAs with two and four strands were synthesized. These structures were used to obtain branched siRNA. The branched siRNA duplexes had similar inhibitory capacity as those of unmodified siRNA duplexes, as deduced from gene silencing experiments of the TNF-α protein. Branched RNAs are considered novel structures for siRNA technology, and they provide an innovative tool for specific gene inhibition. As the method described here is compatible with most RNA modifications described to date, these compounds may be further functionalized to obtain more potent siRNA derivatives and can be attached to suitable delivery systems. |
| format | Article |
| id | doaj-art-08181e3c39a74850b00dc7f8521b4e07 |
| institution | OA Journals |
| issn | 2090-021X |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-08181e3c39a74850b00dc7f8521b4e072025-08-20T02:01:34ZengWileyJournal of Nucleic Acids2090-021X2011-01-01201110.4061/2011/586935586935Branched RNA: A New Architecture for RNA InterferenceAnna Aviñó0Sandra M. Ocampo1José Carlos Perales2Ramon Eritja3Institute for Research in Biomedicine (IRB Barcelona), Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), Networking Centre on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Baldiri Reixac 10, 08028 Barcelona, SpainInstitute for Research in Biomedicine (IRB Barcelona), Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), Networking Centre on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Baldiri Reixac 10, 08028 Barcelona, SpainDepartment of Physiological Sciences, School of Medicine, University of Barcelona (Campus Bellvitge), Feixa Llarga, L'Hospitalet de Llobregat, 08907 Barcelona, SpainInstitute for Research in Biomedicine (IRB Barcelona), Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), Networking Centre on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Baldiri Reixac 10, 08028 Barcelona, SpainBranched RNAs with two and four strands were synthesized. These structures were used to obtain branched siRNA. The branched siRNA duplexes had similar inhibitory capacity as those of unmodified siRNA duplexes, as deduced from gene silencing experiments of the TNF-α protein. Branched RNAs are considered novel structures for siRNA technology, and they provide an innovative tool for specific gene inhibition. As the method described here is compatible with most RNA modifications described to date, these compounds may be further functionalized to obtain more potent siRNA derivatives and can be attached to suitable delivery systems.http://dx.doi.org/10.4061/2011/586935 |
| spellingShingle | Anna Aviñó Sandra M. Ocampo José Carlos Perales Ramon Eritja Branched RNA: A New Architecture for RNA Interference Journal of Nucleic Acids |
| title | Branched RNA: A New Architecture for RNA Interference |
| title_full | Branched RNA: A New Architecture for RNA Interference |
| title_fullStr | Branched RNA: A New Architecture for RNA Interference |
| title_full_unstemmed | Branched RNA: A New Architecture for RNA Interference |
| title_short | Branched RNA: A New Architecture for RNA Interference |
| title_sort | branched rna a new architecture for rna interference |
| url | http://dx.doi.org/10.4061/2011/586935 |
| work_keys_str_mv | AT annaavino branchedrnaanewarchitectureforrnainterference AT sandramocampo branchedrnaanewarchitectureforrnainterference AT josecarlosperales branchedrnaanewarchitectureforrnainterference AT ramoneritja branchedrnaanewarchitectureforrnainterference |