Survey of Potyviruses, Carlaviruses, and Begomoviruses in Potato Cultivation Centers of West, Central, and East Java Provinces, Indonesia
Our knowledge of the molecular data on viruses infecting potato (<i>Solanum tuberosum</i>) and its weeds in Indonesia still needs to be expanded. Thirteen potato leaves, together with one carrot (<i>Daucus carota</i> subsp. <i>sativus</i>) and one <i>Oxalis...
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MDPI AG
2025-06-01
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| Series: | International Journal of Plant Biology |
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| Online Access: | https://www.mdpi.com/2037-0164/16/2/65 |
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| author | Adyatma Irawan Santosa Rossa Wulandari Meyrin Novia Vadilah Erlin Sabila Asista Fatma Kusuma Dedi Mulyadi Intan Berlian Made Getas Pudak Wangi Ade Mahendra Sutejo Ali Çelik |
| author_facet | Adyatma Irawan Santosa Rossa Wulandari Meyrin Novia Vadilah Erlin Sabila Asista Fatma Kusuma Dedi Mulyadi Intan Berlian Made Getas Pudak Wangi Ade Mahendra Sutejo Ali Çelik |
| author_sort | Adyatma Irawan Santosa |
| collection | DOAJ |
| description | Our knowledge of the molecular data on viruses infecting potato (<i>Solanum tuberosum</i>) and its weeds in Indonesia still needs to be expanded. Thirteen potato leaves, together with one carrot (<i>Daucus carota</i> subsp. <i>sativus</i>) and one <i>Oxalis latifolia</i> leaves grew adjacent to potato fields were sampled in West, Central, and East Java Provinces of Indonesia to be tested using RT-PCR with universal primers to monitor the presence of potyviruses, carlaviruses, and begomoviruses in the surveyed regions. According to the test results and BLAST nucleotide analysis, four potato samples were found to be infected by potato virus Y (PVY, <i>Potyvirus</i>). All samples tested negative for carlaviruses and begomoviruses. Both phylogenetic trees constructed by MEGA11 software based on partial coat protein and partial coat protein + 3′-UTR sequences demonstrated that the four new PVY isolates belong to strain N. Nucleotide and amino acid homology analysis determined that all four new and 12 other strain N isolates registered in NBCI GenBank were identical at the observed genome region, suggested the same origin of Indonesian isolates. This study improved our understanding of the variation in PVY isolates in Indonesia and highlighted the need for tight surveillance since the country imports most of its tubers used as planting material. |
| format | Article |
| id | doaj-art-0817f289900245e49afafc2f9c2e379c |
| institution | Kabale University |
| issn | 2037-0164 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Plant Biology |
| spelling | doaj-art-0817f289900245e49afafc2f9c2e379c2025-08-20T03:26:57ZengMDPI AGInternational Journal of Plant Biology2037-01642025-06-011626510.3390/ijpb16020065Survey of Potyviruses, Carlaviruses, and Begomoviruses in Potato Cultivation Centers of West, Central, and East Java Provinces, IndonesiaAdyatma Irawan Santosa0Rossa Wulandari1Meyrin Novia Vadilah2Erlin Sabila3Asista Fatma Kusuma4Dedi Mulyadi5Intan Berlian6Made Getas Pudak Wangi7Ade Mahendra Sutejo8Ali Çelik9Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora No. 1, Sleman 55281, D.I. Yogyakarta, IndonesiaDepartment of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora No. 1, Sleman 55281, D.I. Yogyakarta, IndonesiaDepartment of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora No. 1, Sleman 55281, D.I. Yogyakarta, IndonesiaDepartment of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora No. 1, Sleman 55281, D.I. Yogyakarta, IndonesiaDoctoral Program in Agricultural Science, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora No. 1, Sleman 55281, D.I. Yogyakarta, IndonesiaDepartment of Plant Protection, Faculty of Agriculture, Universitas Riau, Kampus Bina Widya Km. 12.5, Kota Pekanbaru 28292, Riau, IndonesiaDoctoral Program in Agricultural Science, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora No. 1, Sleman 55281, D.I. Yogyakarta, IndonesiaDoctoral Program in Agricultural Science, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora No. 1, Sleman 55281, D.I. Yogyakarta, IndonesiaDoctoral Program in Agricultural Science, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora No. 1, Sleman 55281, D.I. Yogyakarta, IndonesiaDepartment of Plant Protection, Faculty of Agriculture, Bolu Abant İzzet Baysal University, Bolu Merkez 14030, Bolu, TürkiyeOur knowledge of the molecular data on viruses infecting potato (<i>Solanum tuberosum</i>) and its weeds in Indonesia still needs to be expanded. Thirteen potato leaves, together with one carrot (<i>Daucus carota</i> subsp. <i>sativus</i>) and one <i>Oxalis latifolia</i> leaves grew adjacent to potato fields were sampled in West, Central, and East Java Provinces of Indonesia to be tested using RT-PCR with universal primers to monitor the presence of potyviruses, carlaviruses, and begomoviruses in the surveyed regions. According to the test results and BLAST nucleotide analysis, four potato samples were found to be infected by potato virus Y (PVY, <i>Potyvirus</i>). All samples tested negative for carlaviruses and begomoviruses. Both phylogenetic trees constructed by MEGA11 software based on partial coat protein and partial coat protein + 3′-UTR sequences demonstrated that the four new PVY isolates belong to strain N. Nucleotide and amino acid homology analysis determined that all four new and 12 other strain N isolates registered in NBCI GenBank were identical at the observed genome region, suggested the same origin of Indonesian isolates. This study improved our understanding of the variation in PVY isolates in Indonesia and highlighted the need for tight surveillance since the country imports most of its tubers used as planting material.https://www.mdpi.com/2037-0164/16/2/65genetic diversitymolecular testpercentage identityphylogenyplant virus disease |
| spellingShingle | Adyatma Irawan Santosa Rossa Wulandari Meyrin Novia Vadilah Erlin Sabila Asista Fatma Kusuma Dedi Mulyadi Intan Berlian Made Getas Pudak Wangi Ade Mahendra Sutejo Ali Çelik Survey of Potyviruses, Carlaviruses, and Begomoviruses in Potato Cultivation Centers of West, Central, and East Java Provinces, Indonesia International Journal of Plant Biology genetic diversity molecular test percentage identity phylogeny plant virus disease |
| title | Survey of Potyviruses, Carlaviruses, and Begomoviruses in Potato Cultivation Centers of West, Central, and East Java Provinces, Indonesia |
| title_full | Survey of Potyviruses, Carlaviruses, and Begomoviruses in Potato Cultivation Centers of West, Central, and East Java Provinces, Indonesia |
| title_fullStr | Survey of Potyviruses, Carlaviruses, and Begomoviruses in Potato Cultivation Centers of West, Central, and East Java Provinces, Indonesia |
| title_full_unstemmed | Survey of Potyviruses, Carlaviruses, and Begomoviruses in Potato Cultivation Centers of West, Central, and East Java Provinces, Indonesia |
| title_short | Survey of Potyviruses, Carlaviruses, and Begomoviruses in Potato Cultivation Centers of West, Central, and East Java Provinces, Indonesia |
| title_sort | survey of potyviruses carlaviruses and begomoviruses in potato cultivation centers of west central and east java provinces indonesia |
| topic | genetic diversity molecular test percentage identity phylogeny plant virus disease |
| url | https://www.mdpi.com/2037-0164/16/2/65 |
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