Pathogenic mechanisms and molecular features of a novel UL2 gene-deficient duck enteritis virus endemic to China
Duck enteritis virus (DEV) was identified as the etiological agent responsible for an outbreak of morbidity and mortality in adult ducks on a farm in Jiangsu, China. Diagnostic approaches confirmed that the outbreak was caused by the highly pathogenic DEV-JS2024 isolate. The clinical progression of...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-08-01
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| Series: | Virulence |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2025.2547325 |
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| author | Dan Yin Yuehua Gao Mingqing Xu Jianhua Wang Xiaofei Song Zhen Li Jianyun Peng Min Kang Bai Wei Chengdong Yu Yingjuan Qian Yong-Sam Jung Feng Hu Junfeng Lv Zhuoming Qin Yufeng Li |
| author_facet | Dan Yin Yuehua Gao Mingqing Xu Jianhua Wang Xiaofei Song Zhen Li Jianyun Peng Min Kang Bai Wei Chengdong Yu Yingjuan Qian Yong-Sam Jung Feng Hu Junfeng Lv Zhuoming Qin Yufeng Li |
| author_sort | Dan Yin |
| collection | DOAJ |
| description | Duck enteritis virus (DEV) was identified as the etiological agent responsible for an outbreak of morbidity and mortality in adult ducks on a farm in Jiangsu, China. Diagnostic approaches confirmed that the outbreak was caused by the highly pathogenic DEV-JS2024 isolate. The clinical progression of the disease, characterized by lethargy, anorexia, ocular discharge, and high mortality, was accompanied by extensive hemorrhagic lesions in critical organs such as the liver, spleen, lungs, and bursa of Fabricius, consistent with known signs of DEV infection. Genomic analysis of DEV-JS2024 revealed a 45% G+C content and 76 open reading frames. BLASTn analysis revealed that the genome of DEV-JS2024 shares the highest sequence similarity with the Chinese virulent strain CV and the DEV attenuated vaccine strain C-KCE in the database. These results indicate a close genetic relationship between DEV-JS2024 and both the virulent and attenuated strains, suggesting potential similarities in their genomic architecture. Comparative genomic analysis identified 28 nucleotide mutations, including 15 non-synonymous mutations potentially related to virulence factors. The study also highlighted the first reported 528 base pairs deletion in the UL2 gene of a virulent strain, challenging its utility as a marker for distinguishing virulent from attenuated strains. Phylogenetic analysis suggested that DEV-JS2024 May result from recombination between the vaccine and virulent strains, further complicating our understanding of DEV pathogenicity. This study provides new insights into the molecular evolution of DEV and stresses the importance of continued genomic surveillance to enhance vaccine development and control measures for duck plague. |
| format | Article |
| id | doaj-art-080b752a238c4ab5b0332d1f7771e5c4 |
| institution | Kabale University |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-080b752a238c4ab5b0332d1f7771e5c42025-08-20T03:36:01ZengTaylor & Francis GroupVirulence2150-55942150-56082025-08-0110.1080/21505594.2025.2547325Pathogenic mechanisms and molecular features of a novel UL2 gene-deficient duck enteritis virus endemic to ChinaDan Yin0Yuehua Gao1Mingqing Xu2Jianhua Wang3Xiaofei Song4Zhen Li5Jianyun Peng6Min Kang7Bai Wei8Chengdong Yu9Yingjuan Qian10Yong-Sam Jung11Feng Hu12Junfeng Lv13Zhuoming Qin14Yufeng Li15Institute of Poultry Science, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Livestock and Poultry Breeding, Jinan, ChinaInstitute of Poultry Science, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Livestock and Poultry Breeding, Jinan, ChinaShandong Hekangyuan Biological Breeding Co. Ltd, Jinan, ChinaShandong Hekangyuan Biological Breeding Co. Ltd, Jinan, ChinaQilu Animal Health Products Co. Ltd, Jinan, ChinaQilu Animal Health Products Co. Ltd, Jinan, ChinaQilu Animal Health Products Co. Ltd, Jinan, ChinaDepartment of Avian Diseases, College of Veterinary Medicine and Center for Avian Disease, Jeonbuk National University, Iksan, Republic of KoreaDepartment of Avian Diseases, College of Veterinary Medicine and Center for Avian Disease, Jeonbuk National University, Iksan, Republic of KoreaDepartment of Avian Diseases, College of Veterinary Medicine and Center for Avian Disease, Jeonbuk National University, Iksan, Republic of KoreaLaboratory of Emerging Animal Diseases and One Health, The Sanya Institute of Nanjing Agricultural University, Nanjing, ChinaLaboratory of Emerging Animal Diseases and One Health, The Sanya Institute of Nanjing Agricultural University, Nanjing, ChinaInstitute of Poultry Science, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Livestock and Poultry Breeding, Jinan, ChinaInstitute of Poultry Science, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Livestock and Poultry Breeding, Jinan, ChinaInstitute of Poultry Science, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Livestock and Poultry Breeding, Jinan, ChinaInstitute of Poultry Science, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Livestock and Poultry Breeding, Jinan, ChinaDuck enteritis virus (DEV) was identified as the etiological agent responsible for an outbreak of morbidity and mortality in adult ducks on a farm in Jiangsu, China. Diagnostic approaches confirmed that the outbreak was caused by the highly pathogenic DEV-JS2024 isolate. The clinical progression of the disease, characterized by lethargy, anorexia, ocular discharge, and high mortality, was accompanied by extensive hemorrhagic lesions in critical organs such as the liver, spleen, lungs, and bursa of Fabricius, consistent with known signs of DEV infection. Genomic analysis of DEV-JS2024 revealed a 45% G+C content and 76 open reading frames. BLASTn analysis revealed that the genome of DEV-JS2024 shares the highest sequence similarity with the Chinese virulent strain CV and the DEV attenuated vaccine strain C-KCE in the database. These results indicate a close genetic relationship between DEV-JS2024 and both the virulent and attenuated strains, suggesting potential similarities in their genomic architecture. Comparative genomic analysis identified 28 nucleotide mutations, including 15 non-synonymous mutations potentially related to virulence factors. The study also highlighted the first reported 528 base pairs deletion in the UL2 gene of a virulent strain, challenging its utility as a marker for distinguishing virulent from attenuated strains. Phylogenetic analysis suggested that DEV-JS2024 May result from recombination between the vaccine and virulent strains, further complicating our understanding of DEV pathogenicity. This study provides new insights into the molecular evolution of DEV and stresses the importance of continued genomic surveillance to enhance vaccine development and control measures for duck plague.https://www.tandfonline.com/doi/10.1080/21505594.2025.2547325Duck enteritis viruspathogenicitywhole genome sequencingUL2 gene-deficientendemic |
| spellingShingle | Dan Yin Yuehua Gao Mingqing Xu Jianhua Wang Xiaofei Song Zhen Li Jianyun Peng Min Kang Bai Wei Chengdong Yu Yingjuan Qian Yong-Sam Jung Feng Hu Junfeng Lv Zhuoming Qin Yufeng Li Pathogenic mechanisms and molecular features of a novel UL2 gene-deficient duck enteritis virus endemic to China Virulence Duck enteritis virus pathogenicity whole genome sequencing UL2 gene-deficient endemic |
| title | Pathogenic mechanisms and molecular features of a novel UL2 gene-deficient duck enteritis virus endemic to China |
| title_full | Pathogenic mechanisms and molecular features of a novel UL2 gene-deficient duck enteritis virus endemic to China |
| title_fullStr | Pathogenic mechanisms and molecular features of a novel UL2 gene-deficient duck enteritis virus endemic to China |
| title_full_unstemmed | Pathogenic mechanisms and molecular features of a novel UL2 gene-deficient duck enteritis virus endemic to China |
| title_short | Pathogenic mechanisms and molecular features of a novel UL2 gene-deficient duck enteritis virus endemic to China |
| title_sort | pathogenic mechanisms and molecular features of a novel ul2 gene deficient duck enteritis virus endemic to china |
| topic | Duck enteritis virus pathogenicity whole genome sequencing UL2 gene-deficient endemic |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2025.2547325 |
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