<i>Lactobacillus helveticus R0052</i> and <i>Bifidobacterium longum R0175</i> Supplementation: An Exploratory, Randomized, Placebo-Controlled Trial of Endocannabinoid and Inflammatory Responses in Female Dancers

The anandamide (AEA) and lipopolysaccharide (LPS) interaction is gaining attention, but evidence on the influence of probiotics on endocannabinoid system (ECS) biomarkers remains limited. This study (NCT05567653) investigated the effects of 12-week supplementation with <i>Lactobacillus helveti...

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Main Authors: Jakub Wiącek, Karolina Skonieczna-Żydecka, Igor Łoniewski, Chariklia K. Deli, Ioannis G. Fatouros, Athanasios Z. Jamurtas, Dominika Moszczyńska, Joanna Karolkiewicz
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Language:English
Published: MDPI AG 2025-05-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/6/1284
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author Jakub Wiącek
Karolina Skonieczna-Żydecka
Igor Łoniewski
Chariklia K. Deli
Ioannis G. Fatouros
Athanasios Z. Jamurtas
Dominika Moszczyńska
Joanna Karolkiewicz
author_facet Jakub Wiącek
Karolina Skonieczna-Żydecka
Igor Łoniewski
Chariklia K. Deli
Ioannis G. Fatouros
Athanasios Z. Jamurtas
Dominika Moszczyńska
Joanna Karolkiewicz
author_sort Jakub Wiącek
collection DOAJ
description The anandamide (AEA) and lipopolysaccharide (LPS) interaction is gaining attention, but evidence on the influence of probiotics on endocannabinoid system (ECS) biomarkers remains limited. This study (NCT05567653) investigated the effects of 12-week supplementation with <i>Lactobacillus helveticus</i> R0052 and <i>Bifidobacterium longum</i> R0175 on AEA (main outcome) and inflammatory biomarkers in female dancers. Fifteen participants (5 probiotic, 10 placebo) were included in the final analysis. Serum levels of AEA, LPS, and cytokines (tumor necrosis factor-alpha—TNF-α, interleukin-1 beta—IL-1β, and interleukin-10—IL-10) were measured using an ELISA (enzyme-linked immunosorbent assay), and the psychological stress responses were evaluated using the Mini-COPE questionnaire. At the baseline, a correlation between AEA and LPS was observed (Spearman’s r = 0.9677, <i>p</i> < 0.05). After 12 weeks, no statistically significant differences in the AEA, LPS, cytokine levels, or stress-coping strategies were observed between the probiotic and placebo groups (LPS–probiotic: +3.48 EU/L, <i>p</i> = 0.9361; placebo: +56.98 EU/L, <i>p</i> = 0.0694; AEA–probiotic: −1.11 ng/mL, <i>p</i> = 0.9538; placebo: +14.08 ng/mL, <i>p</i> = 0.4749). The direction of change may indicate a trend toward increased inflammation in the absence of probiotics, consistent with patterns described in previous literature. However, these results should be viewed as hypothesis generating and warrant confirmation in larger trials.
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spelling doaj-art-080af5d9fc8d4144b1a971d028f85ba82025-08-20T02:21:07ZengMDPI AGMicroorganisms2076-26072025-05-01136128410.3390/microorganisms13061284<i>Lactobacillus helveticus R0052</i> and <i>Bifidobacterium longum R0175</i> Supplementation: An Exploratory, Randomized, Placebo-Controlled Trial of Endocannabinoid and Inflammatory Responses in Female DancersJakub Wiącek0Karolina Skonieczna-Żydecka1Igor Łoniewski2Chariklia K. Deli3Ioannis G. Fatouros4Athanasios Z. Jamurtas5Dominika Moszczyńska6Joanna Karolkiewicz7Department of Food and Nutrition, Poznan University of Physical Education, 61-871 Poznan, PolandDepartment of Biochemical Research, Pomeranian Medical University, 70-204 Szczecin, PolandSanprobi Sp. z o.o. Sp.K., 70-535 Szczecin, PolandDepartment of Physical Education and Sport Science, University of Thessaly, 38221 Trikala, GreeceDepartment of Physical Education and Sport Science, University of Thessaly, 38221 Trikala, GreeceDepartment of Physical Education and Sport Science, University of Thessaly, 38221 Trikala, GreeceDepartment of Food and Nutrition, Poznan University of Physical Education, 61-871 Poznan, PolandDepartment of Food and Nutrition, Poznan University of Physical Education, 61-871 Poznan, PolandThe anandamide (AEA) and lipopolysaccharide (LPS) interaction is gaining attention, but evidence on the influence of probiotics on endocannabinoid system (ECS) biomarkers remains limited. This study (NCT05567653) investigated the effects of 12-week supplementation with <i>Lactobacillus helveticus</i> R0052 and <i>Bifidobacterium longum</i> R0175 on AEA (main outcome) and inflammatory biomarkers in female dancers. Fifteen participants (5 probiotic, 10 placebo) were included in the final analysis. Serum levels of AEA, LPS, and cytokines (tumor necrosis factor-alpha—TNF-α, interleukin-1 beta—IL-1β, and interleukin-10—IL-10) were measured using an ELISA (enzyme-linked immunosorbent assay), and the psychological stress responses were evaluated using the Mini-COPE questionnaire. At the baseline, a correlation between AEA and LPS was observed (Spearman’s r = 0.9677, <i>p</i> < 0.05). After 12 weeks, no statistically significant differences in the AEA, LPS, cytokine levels, or stress-coping strategies were observed between the probiotic and placebo groups (LPS–probiotic: +3.48 EU/L, <i>p</i> = 0.9361; placebo: +56.98 EU/L, <i>p</i> = 0.0694; AEA–probiotic: −1.11 ng/mL, <i>p</i> = 0.9538; placebo: +14.08 ng/mL, <i>p</i> = 0.4749). The direction of change may indicate a trend toward increased inflammation in the absence of probiotics, consistent with patterns described in previous literature. However, these results should be viewed as hypothesis generating and warrant confirmation in larger trials.https://www.mdpi.com/2076-2607/13/6/1284endocannabinoid systemgut microbiomeanandamidelipopolysaccharideprobioticsdancers
spellingShingle Jakub Wiącek
Karolina Skonieczna-Żydecka
Igor Łoniewski
Chariklia K. Deli
Ioannis G. Fatouros
Athanasios Z. Jamurtas
Dominika Moszczyńska
Joanna Karolkiewicz
<i>Lactobacillus helveticus R0052</i> and <i>Bifidobacterium longum R0175</i> Supplementation: An Exploratory, Randomized, Placebo-Controlled Trial of Endocannabinoid and Inflammatory Responses in Female Dancers
Microorganisms
endocannabinoid system
gut microbiome
anandamide
lipopolysaccharide
probiotics
dancers
title <i>Lactobacillus helveticus R0052</i> and <i>Bifidobacterium longum R0175</i> Supplementation: An Exploratory, Randomized, Placebo-Controlled Trial of Endocannabinoid and Inflammatory Responses in Female Dancers
title_full <i>Lactobacillus helveticus R0052</i> and <i>Bifidobacterium longum R0175</i> Supplementation: An Exploratory, Randomized, Placebo-Controlled Trial of Endocannabinoid and Inflammatory Responses in Female Dancers
title_fullStr <i>Lactobacillus helveticus R0052</i> and <i>Bifidobacterium longum R0175</i> Supplementation: An Exploratory, Randomized, Placebo-Controlled Trial of Endocannabinoid and Inflammatory Responses in Female Dancers
title_full_unstemmed <i>Lactobacillus helveticus R0052</i> and <i>Bifidobacterium longum R0175</i> Supplementation: An Exploratory, Randomized, Placebo-Controlled Trial of Endocannabinoid and Inflammatory Responses in Female Dancers
title_short <i>Lactobacillus helveticus R0052</i> and <i>Bifidobacterium longum R0175</i> Supplementation: An Exploratory, Randomized, Placebo-Controlled Trial of Endocannabinoid and Inflammatory Responses in Female Dancers
title_sort i lactobacillus helveticus r0052 i and i bifidobacterium longum r0175 i supplementation an exploratory randomized placebo controlled trial of endocannabinoid and inflammatory responses in female dancers
topic endocannabinoid system
gut microbiome
anandamide
lipopolysaccharide
probiotics
dancers
url https://www.mdpi.com/2076-2607/13/6/1284
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