Alleviation of Acute Heat Stress in Broiler Chickens by Dietary Supplementation of Polyphenols from Shredded, Steam-Exploded Pine Particles
Reducing the detrimental effects of heat stress (HS) in poultry is essential to minimize production losses. The present study evaluates the effects of dietary polyphenols prepared from underutilized wood byproducts on the growth, gut health, and cecal microbiota in broilers subjected to acute heat s...
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2025-01-01
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| author | Akshat Goel Chris-Major Ncho Chae-Mi Jeong Vaishali Gupta Ji-Young Jung Si-Young Ha Jae-Kyung Yang Yang-Ho Choi |
| author_facet | Akshat Goel Chris-Major Ncho Chae-Mi Jeong Vaishali Gupta Ji-Young Jung Si-Young Ha Jae-Kyung Yang Yang-Ho Choi |
| author_sort | Akshat Goel |
| collection | DOAJ |
| description | Reducing the detrimental effects of heat stress (HS) in poultry is essential to minimize production losses. The present study evaluates the effects of dietary polyphenols prepared from underutilized wood byproducts on the growth, gut health, and cecal microbiota in broilers subjected to acute heat stress (AHS). One hundred eight one-day-old Indian River broilers were fed with 0%, 0.5%, or 1% polyphenols from shredded, steam-exploded pine particles (PSPP) in their diet. On the 37th day, forty birds were equally distributed among four groups containing either a control diet at thermoneutral temperatures (NT0%) or AHS temperatures with 0% (AHS0%), 0.5% (AHS0.5%), and 1% (AHS1%) PSPP-supplemented diets. The temperature in the NT room was maintained at 21.0 °C, while, in the AHS room, it was increased to 31 °C. AHS negatively influenced performance parameters and increased rectal temperature (RT) in broilers. The AHS0% group showed a higher expression of NOX4, HSP-70, and HSP-90 genes, while the expression was lower in PSPP-supplemented birds. In the jejunum, mRNA expression of <i>SOD</i> was increased in all the birds under AHS compared to NT. The expression of the <i>CLDN1</i> and <i>ZO2</i> genes was increased in AHS0%, while that of the <i>ZO1</i> and <i>MUC2</i> genes was increased in PSPP-supplemented birds. HS tends to increase <i>TLR2</i> and <i>TLR4</i> gene expression in chickens. The significantly modified genera were <i>Bariatricus</i>, <i>Sporobacter</i>, <i>Sporanaerobacter</i>, and <i>Natranaerovirga</i>. Concludingly, AHS negatively influences the performance parameters, RT, stress, gut-health-related genes, and pathogenic penetration, but PSPP supplementation reduces its bad impact by overcoming the stress and gut-health-related genes, increasing favorable bacterial abundance and reducing pathogenic penetration in chickens. |
| format | Article |
| id | doaj-art-080a9c999d06407ba758cb36dbdc089f |
| institution | OA Journals |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Microorganisms |
| spelling | doaj-art-080a9c999d06407ba758cb36dbdc089f2025-08-20T02:03:40ZengMDPI AGMicroorganisms2076-26072025-01-0113223510.3390/microorganisms13020235Alleviation of Acute Heat Stress in Broiler Chickens by Dietary Supplementation of Polyphenols from Shredded, Steam-Exploded Pine ParticlesAkshat Goel0Chris-Major Ncho1Chae-Mi Jeong2Vaishali Gupta3Ji-Young Jung4Si-Young Ha5Jae-Kyung Yang6Yang-Ho Choi7Division of Animal Science, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Animal Science, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Animal Science, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Animal Science, Gyeongsang National University, Jinju 52828, Republic of KoreaInstitute of Agriculture and Life Sciences, Gyeongsang National University, Jinju 52828, Republic of KoreaDepartment of Environmental Materials Science, Gyeongsang National University, Jinju 52828, Republic of KoreaInstitute of Agriculture and Life Sciences, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Animal Science, Gyeongsang National University, Jinju 52828, Republic of KoreaReducing the detrimental effects of heat stress (HS) in poultry is essential to minimize production losses. The present study evaluates the effects of dietary polyphenols prepared from underutilized wood byproducts on the growth, gut health, and cecal microbiota in broilers subjected to acute heat stress (AHS). One hundred eight one-day-old Indian River broilers were fed with 0%, 0.5%, or 1% polyphenols from shredded, steam-exploded pine particles (PSPP) in their diet. On the 37th day, forty birds were equally distributed among four groups containing either a control diet at thermoneutral temperatures (NT0%) or AHS temperatures with 0% (AHS0%), 0.5% (AHS0.5%), and 1% (AHS1%) PSPP-supplemented diets. The temperature in the NT room was maintained at 21.0 °C, while, in the AHS room, it was increased to 31 °C. AHS negatively influenced performance parameters and increased rectal temperature (RT) in broilers. The AHS0% group showed a higher expression of NOX4, HSP-70, and HSP-90 genes, while the expression was lower in PSPP-supplemented birds. In the jejunum, mRNA expression of <i>SOD</i> was increased in all the birds under AHS compared to NT. The expression of the <i>CLDN1</i> and <i>ZO2</i> genes was increased in AHS0%, while that of the <i>ZO1</i> and <i>MUC2</i> genes was increased in PSPP-supplemented birds. HS tends to increase <i>TLR2</i> and <i>TLR4</i> gene expression in chickens. The significantly modified genera were <i>Bariatricus</i>, <i>Sporobacter</i>, <i>Sporanaerobacter</i>, and <i>Natranaerovirga</i>. Concludingly, AHS negatively influences the performance parameters, RT, stress, gut-health-related genes, and pathogenic penetration, but PSPP supplementation reduces its bad impact by overcoming the stress and gut-health-related genes, increasing favorable bacterial abundance and reducing pathogenic penetration in chickens.https://www.mdpi.com/2076-2607/13/2/235acute heat stressbroilerscecum metagenomegene expressionpolyphenols from steam-exploded pine particles |
| spellingShingle | Akshat Goel Chris-Major Ncho Chae-Mi Jeong Vaishali Gupta Ji-Young Jung Si-Young Ha Jae-Kyung Yang Yang-Ho Choi Alleviation of Acute Heat Stress in Broiler Chickens by Dietary Supplementation of Polyphenols from Shredded, Steam-Exploded Pine Particles Microorganisms acute heat stress broilers cecum metagenome gene expression polyphenols from steam-exploded pine particles |
| title | Alleviation of Acute Heat Stress in Broiler Chickens by Dietary Supplementation of Polyphenols from Shredded, Steam-Exploded Pine Particles |
| title_full | Alleviation of Acute Heat Stress in Broiler Chickens by Dietary Supplementation of Polyphenols from Shredded, Steam-Exploded Pine Particles |
| title_fullStr | Alleviation of Acute Heat Stress in Broiler Chickens by Dietary Supplementation of Polyphenols from Shredded, Steam-Exploded Pine Particles |
| title_full_unstemmed | Alleviation of Acute Heat Stress in Broiler Chickens by Dietary Supplementation of Polyphenols from Shredded, Steam-Exploded Pine Particles |
| title_short | Alleviation of Acute Heat Stress in Broiler Chickens by Dietary Supplementation of Polyphenols from Shredded, Steam-Exploded Pine Particles |
| title_sort | alleviation of acute heat stress in broiler chickens by dietary supplementation of polyphenols from shredded steam exploded pine particles |
| topic | acute heat stress broilers cecum metagenome gene expression polyphenols from steam-exploded pine particles |
| url | https://www.mdpi.com/2076-2607/13/2/235 |
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