Oxidative Stress Induced Damage and Early Senescence in Preterm Placenta
Introduction. Senescent cells have been demonstrated to release High Mobility Group Box 1 (HMGB1) which induces labor through an inflammatory pathway. This research is aimed at demonstrating whether telomere shortening, proinflammatory HMGB1, and oxidative damage marker 8-OHdG play a role in the pla...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Journal of Pregnancy |
| Online Access: | http://dx.doi.org/10.1155/2021/9923761 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546036823883776 |
|---|---|
| author | Yudianto Budi Saroyo Noroyono Wibowo Rima Irwinda Ani Retno Prijanti Evy Yunihastuti Saptawati Bardosono Sofie Rifayani Krisnadi Putri Indah Permata Stephanie Wijaya Victor Prana Andika Santawi |
| author_facet | Yudianto Budi Saroyo Noroyono Wibowo Rima Irwinda Ani Retno Prijanti Evy Yunihastuti Saptawati Bardosono Sofie Rifayani Krisnadi Putri Indah Permata Stephanie Wijaya Victor Prana Andika Santawi |
| author_sort | Yudianto Budi Saroyo |
| collection | DOAJ |
| description | Introduction. Senescent cells have been demonstrated to release High Mobility Group Box 1 (HMGB1) which induces labor through an inflammatory pathway. This research is aimed at demonstrating whether telomere shortening, proinflammatory HMGB1, and oxidative damage marker 8-OHdG play a role in the placenta of preterm birth in comparison to term birth. Method. A cross-sectional study on 67 full thickness of the placenta obtained from mothers with term and preterm birth. Mothers with clinical signs of infection (fever>38°C, leukocytosis>18000/μL, or abnormal vaginal discharge) and other pregnancy complications were excluded. Real-time polymerase chain reaction was performed to measure T/S ratio and ELISA quantification to measure the amount of HMGB1 and 8-OHdG. Result. A total of 34 placentas from preterm and 33 placentas from term birth were examined. Maternal characteristics were comparable between the two groups. There were no statistical difference of T/S ratio (p=0.181), HMGB1 (p=0.119), and 8-OHdG (p=0.144) between the preterm and term groups. HMGB1 was moderately correlated with 8-OHdG (r=0.314). Telomere T/S ratio of the placenta did not differ between preterm and term labor despite difference in gestational age, suggesting earlier shortening in the preterm group. It is possible that critical telomere length has been achieved in both term and preterm placenta that warrants labor through senescence process. The result of our study also showed that HMGB1 was not correlated to telomere length, due to the fact that HMGB1 is not upregulated until the critical length of telomere for senescence is exhibited. Conclusion. Similar telomere length might be exhibited due to early telomere shortening in preterm birth that mimics the term placenta. The relationship between placental telomere shortening and HMGB1 release remains to be uncovered. Further research is needed to discover the factors leading to early telomere shortening in the placenta of preterm birth. |
| format | Article |
| id | doaj-art-0808781191e44d0ab0ada6b2c526db3e |
| institution | Kabale University |
| issn | 2090-2727 2090-2735 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Pregnancy |
| spelling | doaj-art-0808781191e44d0ab0ada6b2c526db3e2025-02-03T07:23:57ZengWileyJournal of Pregnancy2090-27272090-27352021-01-01202110.1155/2021/99237619923761Oxidative Stress Induced Damage and Early Senescence in Preterm PlacentaYudianto Budi Saroyo0Noroyono Wibowo1Rima Irwinda2Ani Retno Prijanti3Evy Yunihastuti4Saptawati Bardosono5Sofie Rifayani Krisnadi6Putri Indah Permata7Stephanie Wijaya8Victor Prana Andika Santawi9Maternal Fetal Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia/Cipto-Mangunkusumo Hospital, IndonesiaMaternal Fetal Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia/Cipto-Mangunkusumo Hospital, IndonesiaMaternal Fetal Division, Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia/Cipto-Mangunkusumo Hospital, IndonesiaDepartment of Biochemistry and Molecular Biology, Faculty of Medicine Universitas Indonesia/Cipto-Mangunkusumo Hospital, IndonesiaDivision of Allergy Immunology, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia/Cipto-Mangunkusumo Hospital, IndonesiaDepartment of Clinical Nutrition, Universitas Indonesia/Cipto-Mangunkusumo Hospital, IndonesiaDepartment of Obstetrics and Gynecology, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital, IndonesiaDepartment of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, IndonesiaDepartment of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, IndonesiaDepartment of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, IndonesiaIntroduction. Senescent cells have been demonstrated to release High Mobility Group Box 1 (HMGB1) which induces labor through an inflammatory pathway. This research is aimed at demonstrating whether telomere shortening, proinflammatory HMGB1, and oxidative damage marker 8-OHdG play a role in the placenta of preterm birth in comparison to term birth. Method. A cross-sectional study on 67 full thickness of the placenta obtained from mothers with term and preterm birth. Mothers with clinical signs of infection (fever>38°C, leukocytosis>18000/μL, or abnormal vaginal discharge) and other pregnancy complications were excluded. Real-time polymerase chain reaction was performed to measure T/S ratio and ELISA quantification to measure the amount of HMGB1 and 8-OHdG. Result. A total of 34 placentas from preterm and 33 placentas from term birth were examined. Maternal characteristics were comparable between the two groups. There were no statistical difference of T/S ratio (p=0.181), HMGB1 (p=0.119), and 8-OHdG (p=0.144) between the preterm and term groups. HMGB1 was moderately correlated with 8-OHdG (r=0.314). Telomere T/S ratio of the placenta did not differ between preterm and term labor despite difference in gestational age, suggesting earlier shortening in the preterm group. It is possible that critical telomere length has been achieved in both term and preterm placenta that warrants labor through senescence process. The result of our study also showed that HMGB1 was not correlated to telomere length, due to the fact that HMGB1 is not upregulated until the critical length of telomere for senescence is exhibited. Conclusion. Similar telomere length might be exhibited due to early telomere shortening in preterm birth that mimics the term placenta. The relationship between placental telomere shortening and HMGB1 release remains to be uncovered. Further research is needed to discover the factors leading to early telomere shortening in the placenta of preterm birth.http://dx.doi.org/10.1155/2021/9923761 |
| spellingShingle | Yudianto Budi Saroyo Noroyono Wibowo Rima Irwinda Ani Retno Prijanti Evy Yunihastuti Saptawati Bardosono Sofie Rifayani Krisnadi Putri Indah Permata Stephanie Wijaya Victor Prana Andika Santawi Oxidative Stress Induced Damage and Early Senescence in Preterm Placenta Journal of Pregnancy |
| title | Oxidative Stress Induced Damage and Early Senescence in Preterm Placenta |
| title_full | Oxidative Stress Induced Damage and Early Senescence in Preterm Placenta |
| title_fullStr | Oxidative Stress Induced Damage and Early Senescence in Preterm Placenta |
| title_full_unstemmed | Oxidative Stress Induced Damage and Early Senescence in Preterm Placenta |
| title_short | Oxidative Stress Induced Damage and Early Senescence in Preterm Placenta |
| title_sort | oxidative stress induced damage and early senescence in preterm placenta |
| url | http://dx.doi.org/10.1155/2021/9923761 |
| work_keys_str_mv | AT yudiantobudisaroyo oxidativestressinduceddamageandearlysenescenceinpretermplacenta AT noroyonowibowo oxidativestressinduceddamageandearlysenescenceinpretermplacenta AT rimairwinda oxidativestressinduceddamageandearlysenescenceinpretermplacenta AT aniretnoprijanti oxidativestressinduceddamageandearlysenescenceinpretermplacenta AT evyyunihastuti oxidativestressinduceddamageandearlysenescenceinpretermplacenta AT saptawatibardosono oxidativestressinduceddamageandearlysenescenceinpretermplacenta AT sofierifayanikrisnadi oxidativestressinduceddamageandearlysenescenceinpretermplacenta AT putriindahpermata oxidativestressinduceddamageandearlysenescenceinpretermplacenta AT stephaniewijaya oxidativestressinduceddamageandearlysenescenceinpretermplacenta AT victorpranaandikasantawi oxidativestressinduceddamageandearlysenescenceinpretermplacenta |