Application of Multivariate Statistical Analysis to Simultaneous Spectrophotometric Enzymatic Determination of Glucose and Cholesterol in Serum Samples
A method using UV-Vis spectroscopy and multivariate tools for simultaneous determination of glucose and cholesterol was developed in this paper. The method is based on the development of the reaction between the analytes (cholesterol and glucose) and enzymatic reagents. The spectra were analyzed by...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/7532687 |
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| author | Jessica Torres-Gamez Jose A. Rodriguez M. Elena Paez-Hernandez Carlos A. Galan-Vidal |
| author_facet | Jessica Torres-Gamez Jose A. Rodriguez M. Elena Paez-Hernandez Carlos A. Galan-Vidal |
| author_sort | Jessica Torres-Gamez |
| collection | DOAJ |
| description | A method using UV-Vis spectroscopy and multivariate tools for simultaneous determination of glucose and cholesterol was developed in this paper. The method is based on the development of the reaction between the analytes (cholesterol and glucose) and enzymatic reagents. The spectra were analyzed by partial least squares regression and artificial neural networks. The precision estimated between nominal and calculate concentration demonstrate that artificial neural network model was adequate to quantify both analytes in serum samples, since the % relative error obtained was in the interval from 5.1 to 8.3. The proposed model was applied to analyze blood serum samples, and the results are similar compared to those obtained employing the reference method. |
| format | Article |
| id | doaj-art-080149e84f76453c8caa52d559f61071 |
| institution | DOAJ |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-080149e84f76453c8caa52d559f610712025-08-20T03:17:31ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792019-01-01201910.1155/2019/75326877532687Application of Multivariate Statistical Analysis to Simultaneous Spectrophotometric Enzymatic Determination of Glucose and Cholesterol in Serum SamplesJessica Torres-Gamez0Jose A. Rodriguez1M. Elena Paez-Hernandez2Carlos A. Galan-Vidal3Universidad Autonoma del Estado de Hidalgo, Area Academica de Quimica, Carr. Pachuca-Tulancingo Km. 4.5, 42184 Mineral de la Reforma, HGO, MexicoUniversidad Autonoma del Estado de Hidalgo, Area Academica de Quimica, Carr. Pachuca-Tulancingo Km. 4.5, 42184 Mineral de la Reforma, HGO, MexicoUniversidad Autonoma del Estado de Hidalgo, Area Academica de Quimica, Carr. Pachuca-Tulancingo Km. 4.5, 42184 Mineral de la Reforma, HGO, MexicoUniversidad Autonoma del Estado de Hidalgo, Area Academica de Quimica, Carr. Pachuca-Tulancingo Km. 4.5, 42184 Mineral de la Reforma, HGO, MexicoA method using UV-Vis spectroscopy and multivariate tools for simultaneous determination of glucose and cholesterol was developed in this paper. The method is based on the development of the reaction between the analytes (cholesterol and glucose) and enzymatic reagents. The spectra were analyzed by partial least squares regression and artificial neural networks. The precision estimated between nominal and calculate concentration demonstrate that artificial neural network model was adequate to quantify both analytes in serum samples, since the % relative error obtained was in the interval from 5.1 to 8.3. The proposed model was applied to analyze blood serum samples, and the results are similar compared to those obtained employing the reference method.http://dx.doi.org/10.1155/2019/7532687 |
| spellingShingle | Jessica Torres-Gamez Jose A. Rodriguez M. Elena Paez-Hernandez Carlos A. Galan-Vidal Application of Multivariate Statistical Analysis to Simultaneous Spectrophotometric Enzymatic Determination of Glucose and Cholesterol in Serum Samples International Journal of Analytical Chemistry |
| title | Application of Multivariate Statistical Analysis to Simultaneous Spectrophotometric Enzymatic Determination of Glucose and Cholesterol in Serum Samples |
| title_full | Application of Multivariate Statistical Analysis to Simultaneous Spectrophotometric Enzymatic Determination of Glucose and Cholesterol in Serum Samples |
| title_fullStr | Application of Multivariate Statistical Analysis to Simultaneous Spectrophotometric Enzymatic Determination of Glucose and Cholesterol in Serum Samples |
| title_full_unstemmed | Application of Multivariate Statistical Analysis to Simultaneous Spectrophotometric Enzymatic Determination of Glucose and Cholesterol in Serum Samples |
| title_short | Application of Multivariate Statistical Analysis to Simultaneous Spectrophotometric Enzymatic Determination of Glucose and Cholesterol in Serum Samples |
| title_sort | application of multivariate statistical analysis to simultaneous spectrophotometric enzymatic determination of glucose and cholesterol in serum samples |
| url | http://dx.doi.org/10.1155/2019/7532687 |
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