Evaluation of Serum Testosterone, Progesterone, Seminal Antisperm Antibody, and Fructose Levels among Jordanian Males with a History of Infertility
Due to the biochemical complexity of seminal fluid, we attempt to study the possible correlation between fructose, which is secreted under the effect of androgen hormone, and autoimmunity, which might play a role in varicocele associated infertility, in reducing sperm motility. Seminal fructose, ant...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2010/409640 |
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| author | Hala I. Al-Daghistani Abdul-Wahab R. Hamad Muna Abdel-Dayem Mohammad Al-Swaifi Mohammad Abu Zaid |
| author_facet | Hala I. Al-Daghistani Abdul-Wahab R. Hamad Muna Abdel-Dayem Mohammad Al-Swaifi Mohammad Abu Zaid |
| author_sort | Hala I. Al-Daghistani |
| collection | DOAJ |
| description | Due to the biochemical complexity of seminal fluid, we attempt to study the possible correlation between fructose, which is secreted under the effect of androgen hormone, and autoimmunity, which might play a role in varicocele associated infertility, in reducing sperm motility. Seminal fructose, antisperm antibodies (ASAs) and blood steroids hormones (testosterone and progesterone) levels were measured in 66 infertile males with varicocele and 84 without varicocele referred for fertility treatment. Seminal analysis was performed with biochemical measurements of seminal fructose and mixed agglutination reaction (MAR) for ASA. Serum levels of progesterone and testosterone were estimated using a competitive chemoluminescent enzyme immunoassay. The mean values for serum testosterone were 380.74±24.331, 365.9±16.55, and 367.5±21.8 ng/dl, progesterone 0.325±0.243, 0.341±0.022, and 0.357 ± 0.0306 ng/ml, and seminal plasma fructose 359.6 ± 26.75, 315.6 ± 13.08, and 332.08 ± 24.38 mg/dl in males with varicocele, without varicocele, and fertile males, respectively. A significant high level of testosterone was observed within varicocele group (P=.001). This result showed that testosterone may play a role as an infertility determinant in subjects with varicocele. ASA was detected in 18 (26.47%) of cases with varicocele, 20 (38.46%) without varicocele, and in 16 (32.0%) fertile men. Cases with ASAs associated with low sperm motility morphology. An inverse correlation between sperm-bound antibodies and viscosity has been shown (P=.017). ASA showed some significant inverse relations with ages, durations of infertility, and viscosity (P<.05). In addition, a significant correlation was observed between ASA positive seminal plasma and testosterone concentration among infertile cases (with or without varicocele) and fertile (P<.05). Our results suggest a relationship between testicular steroid hormone levels with autoimmunity and sperm antibodies which influence the motility of ejaculated spermatozoa among Jordanian infertile males. |
| format | Article |
| id | doaj-art-07fb9da83cf4430593f17fadd6cd4452 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-07fb9da83cf4430593f17fadd6cd44522025-08-20T03:55:27ZengWileyBiochemistry Research International2090-22472090-22552010-01-01201010.1155/2010/409640409640Evaluation of Serum Testosterone, Progesterone, Seminal Antisperm Antibody, and Fructose Levels among Jordanian Males with a History of InfertilityHala I. Al-Daghistani0Abdul-Wahab R. Hamad1Muna Abdel-Dayem2Mohammad Al-Swaifi3Mohammad Abu Zaid4Department of Medical Allied Sciences, Al-Salt University College, Al-Balqa Applied University, Al-Salt 19117, JordanDepartment of Medical Allied Sciences, Zarka University College, Al-Balqa Applied University, Zarka 13115, JordanMedical Hussein City Hospital, Amman 11855, JordanMedical Hussein City Hospital, Amman 11855, JordanMedical Hussein City Hospital, Amman 11855, JordanDue to the biochemical complexity of seminal fluid, we attempt to study the possible correlation between fructose, which is secreted under the effect of androgen hormone, and autoimmunity, which might play a role in varicocele associated infertility, in reducing sperm motility. Seminal fructose, antisperm antibodies (ASAs) and blood steroids hormones (testosterone and progesterone) levels were measured in 66 infertile males with varicocele and 84 without varicocele referred for fertility treatment. Seminal analysis was performed with biochemical measurements of seminal fructose and mixed agglutination reaction (MAR) for ASA. Serum levels of progesterone and testosterone were estimated using a competitive chemoluminescent enzyme immunoassay. The mean values for serum testosterone were 380.74±24.331, 365.9±16.55, and 367.5±21.8 ng/dl, progesterone 0.325±0.243, 0.341±0.022, and 0.357 ± 0.0306 ng/ml, and seminal plasma fructose 359.6 ± 26.75, 315.6 ± 13.08, and 332.08 ± 24.38 mg/dl in males with varicocele, without varicocele, and fertile males, respectively. A significant high level of testosterone was observed within varicocele group (P=.001). This result showed that testosterone may play a role as an infertility determinant in subjects with varicocele. ASA was detected in 18 (26.47%) of cases with varicocele, 20 (38.46%) without varicocele, and in 16 (32.0%) fertile men. Cases with ASAs associated with low sperm motility morphology. An inverse correlation between sperm-bound antibodies and viscosity has been shown (P=.017). ASA showed some significant inverse relations with ages, durations of infertility, and viscosity (P<.05). In addition, a significant correlation was observed between ASA positive seminal plasma and testosterone concentration among infertile cases (with or without varicocele) and fertile (P<.05). Our results suggest a relationship between testicular steroid hormone levels with autoimmunity and sperm antibodies which influence the motility of ejaculated spermatozoa among Jordanian infertile males.http://dx.doi.org/10.1155/2010/409640 |
| spellingShingle | Hala I. Al-Daghistani Abdul-Wahab R. Hamad Muna Abdel-Dayem Mohammad Al-Swaifi Mohammad Abu Zaid Evaluation of Serum Testosterone, Progesterone, Seminal Antisperm Antibody, and Fructose Levels among Jordanian Males with a History of Infertility Biochemistry Research International |
| title | Evaluation of Serum Testosterone, Progesterone, Seminal Antisperm Antibody, and Fructose Levels among Jordanian Males with a History of Infertility |
| title_full | Evaluation of Serum Testosterone, Progesterone, Seminal Antisperm Antibody, and Fructose Levels among Jordanian Males with a History of Infertility |
| title_fullStr | Evaluation of Serum Testosterone, Progesterone, Seminal Antisperm Antibody, and Fructose Levels among Jordanian Males with a History of Infertility |
| title_full_unstemmed | Evaluation of Serum Testosterone, Progesterone, Seminal Antisperm Antibody, and Fructose Levels among Jordanian Males with a History of Infertility |
| title_short | Evaluation of Serum Testosterone, Progesterone, Seminal Antisperm Antibody, and Fructose Levels among Jordanian Males with a History of Infertility |
| title_sort | evaluation of serum testosterone progesterone seminal antisperm antibody and fructose levels among jordanian males with a history of infertility |
| url | http://dx.doi.org/10.1155/2010/409640 |
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