Genotyping and Molecular Characterization of VP6 and NSP4 Genes of Unusual Rotavirus Group A Isolated from Children with Acute Gastroenteritis
Group A rotavirus (RVA), which causes acute gastroenteritis (AGE) in children worldwide, is categorized mainly based on VP7 (genotype G) and VP4 (genotype P) genes. Genotypes that circulate at <1% are considered unusual. Important genes also include VP6 (genotype I) and NSP4 (genotype E). VP6 est...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2024/3263228 |
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| author | Charilaos Dellis Elizabeth-Barbara Tatsi Dimitra-Maria Koukou Filippos Filippatos Evangelia-Eirini Vetouli Emmanouil Zoumakis Athanasios Michos Vasiliki Syriopoulou |
| author_facet | Charilaos Dellis Elizabeth-Barbara Tatsi Dimitra-Maria Koukou Filippos Filippatos Evangelia-Eirini Vetouli Emmanouil Zoumakis Athanasios Michos Vasiliki Syriopoulou |
| author_sort | Charilaos Dellis |
| collection | DOAJ |
| description | Group A rotavirus (RVA), which causes acute gastroenteritis (AGE) in children worldwide, is categorized mainly based on VP7 (genotype G) and VP4 (genotype P) genes. Genotypes that circulate at <1% are considered unusual. Important genes also include VP6 (genotype I) and NSP4 (genotype E). VP6 establishes the group and affects immunogenicity, while NSP4, as an enterotoxin, is responsible for the clinical symptoms. The aim of this study was to genotype the VP6 and NSP4 genes and molecularly characterize the NSP4 and VP6 genes of unusual RVA. Unusual RVA strains extracted from fecal samples of children ≤16 years with AGE were genotyped in VP6 and NSP4 genes with Sanger sequencing. In a 15-year period (2007–2021), 54.8% (34/62) of unusual RVA were successfully I and E genotyped. Three different I and E genotypes were identified; I2 (73.5%, 25/34) and E2 (35.3%, 12/34) were the most common. E3 genotype was detected from 2017 onwards. The uncommon combination of I2-E3 was found in 26.5% (9/34) of the strains and G3-P[9]-I2-E3 remained the most frequent G-P-I-E combination (20.6%, 7/34). Children infected with RVA E2 strains had a statistically higher frequency of dehydration (50%) than those infected with RVA E3 strains (p=0.019). Multiple substitutions were detected in NSP4, but their functional effect remains unknown. The result indicates the genetic diversity of RVA strains. Continuous surveillance of the RVA based on the whole genome will provide better knowledge of its evolution. |
| format | Article |
| id | doaj-art-07ee0181474d4636bec08795328f58ac |
| institution | Kabale University |
| issn | 1687-8647 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-07ee0181474d4636bec08795328f58ac2025-02-03T01:30:21ZengWileyAdvances in Virology1687-86472024-01-01202410.1155/2024/3263228Genotyping and Molecular Characterization of VP6 and NSP4 Genes of Unusual Rotavirus Group A Isolated from Children with Acute GastroenteritisCharilaos Dellis0Elizabeth-Barbara Tatsi1Dimitra-Maria Koukou2Filippos Filippatos3Evangelia-Eirini Vetouli4Emmanouil Zoumakis5Athanasios Michos6Vasiliki Syriopoulou7First Department of PediatricsFirst Department of PediatricsFirst Department of PediatricsFirst Department of PediatricsDepartment of MicrobiologyFirst Department of PediatricsFirst Department of PediatricsFirst Department of PediatricsGroup A rotavirus (RVA), which causes acute gastroenteritis (AGE) in children worldwide, is categorized mainly based on VP7 (genotype G) and VP4 (genotype P) genes. Genotypes that circulate at <1% are considered unusual. Important genes also include VP6 (genotype I) and NSP4 (genotype E). VP6 establishes the group and affects immunogenicity, while NSP4, as an enterotoxin, is responsible for the clinical symptoms. The aim of this study was to genotype the VP6 and NSP4 genes and molecularly characterize the NSP4 and VP6 genes of unusual RVA. Unusual RVA strains extracted from fecal samples of children ≤16 years with AGE were genotyped in VP6 and NSP4 genes with Sanger sequencing. In a 15-year period (2007–2021), 54.8% (34/62) of unusual RVA were successfully I and E genotyped. Three different I and E genotypes were identified; I2 (73.5%, 25/34) and E2 (35.3%, 12/34) were the most common. E3 genotype was detected from 2017 onwards. The uncommon combination of I2-E3 was found in 26.5% (9/34) of the strains and G3-P[9]-I2-E3 remained the most frequent G-P-I-E combination (20.6%, 7/34). Children infected with RVA E2 strains had a statistically higher frequency of dehydration (50%) than those infected with RVA E3 strains (p=0.019). Multiple substitutions were detected in NSP4, but their functional effect remains unknown. The result indicates the genetic diversity of RVA strains. Continuous surveillance of the RVA based on the whole genome will provide better knowledge of its evolution.http://dx.doi.org/10.1155/2024/3263228 |
| spellingShingle | Charilaos Dellis Elizabeth-Barbara Tatsi Dimitra-Maria Koukou Filippos Filippatos Evangelia-Eirini Vetouli Emmanouil Zoumakis Athanasios Michos Vasiliki Syriopoulou Genotyping and Molecular Characterization of VP6 and NSP4 Genes of Unusual Rotavirus Group A Isolated from Children with Acute Gastroenteritis Advances in Virology |
| title | Genotyping and Molecular Characterization of VP6 and NSP4 Genes of Unusual Rotavirus Group A Isolated from Children with Acute Gastroenteritis |
| title_full | Genotyping and Molecular Characterization of VP6 and NSP4 Genes of Unusual Rotavirus Group A Isolated from Children with Acute Gastroenteritis |
| title_fullStr | Genotyping and Molecular Characterization of VP6 and NSP4 Genes of Unusual Rotavirus Group A Isolated from Children with Acute Gastroenteritis |
| title_full_unstemmed | Genotyping and Molecular Characterization of VP6 and NSP4 Genes of Unusual Rotavirus Group A Isolated from Children with Acute Gastroenteritis |
| title_short | Genotyping and Molecular Characterization of VP6 and NSP4 Genes of Unusual Rotavirus Group A Isolated from Children with Acute Gastroenteritis |
| title_sort | genotyping and molecular characterization of vp6 and nsp4 genes of unusual rotavirus group a isolated from children with acute gastroenteritis |
| url | http://dx.doi.org/10.1155/2024/3263228 |
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