Identification of powdery mildew on Prunus rufoides in China, caused by Podosphaera prunigena

Prunus rufoides is a deciduous wild tree, native to China, which is also used as an ornamental. From late March to December in the years 2018 to 2023, P. rufoides plants growing in Siming Mountain (29°71’08”N, 121°15’12”E), Ningbo, Zhejiang Province, were severely affected by powdery mildew. The dis...

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Main Authors: Zhilong WANG, Wen LI, Yueqiu HE, Feng LIU, Kai FENG, Liang LIU, Tao FU, Qi YE, Guoliang WANG
Format: Article
Language:English
Published: Firenze University Press 2024-12-01
Series:Phytopathologia Mediterranea
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Online Access:https://oajournals.fupress.net/index.php/pm/article/view/15560
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author Zhilong WANG
Wen LI
Yueqiu HE
Feng LIU
Kai FENG
Liang LIU
Tao FU
Qi YE
Guoliang WANG
author_facet Zhilong WANG
Wen LI
Yueqiu HE
Feng LIU
Kai FENG
Liang LIU
Tao FU
Qi YE
Guoliang WANG
author_sort Zhilong WANG
collection DOAJ
description Prunus rufoides is a deciduous wild tree, native to China, which is also used as an ornamental. From late March to December in the years 2018 to 2023, P. rufoides plants growing in Siming Mountain (29°71’08”N, 121°15’12”E), Ningbo, Zhejiang Province, were severely affected by powdery mildew. The disease initially emerged in late March each year, and was characterized as white, irregular mycelial patches on the adaxial surfaces of young leaves. Between July and August, the powdery mildew colonies on affected parts of leaves disappeared, leaving only irregular yellow-brown spots. The disease recurred in September and persisted until late December. Chasmothecia containing asci and ascospores were observed on the leaves in December. Morphological analyses of the chasmothecia indicated a Podosphaera sp. as the causative agent. Molecular identification, based on the internal transcribed spacer (ITS) region (primers ITS4/ITS5) confirmed the identity of the pathogen as Podosphaera prunigena. Koch’s postulates were fulfilled by inoculation tests in which the same pathogen was identified from the inoculated leaf tissue. This study represents the first report confirming the powdery mildew disease on P. rufoides in China to be caused by P. prunigena.
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issn 0031-9465
1593-2095
language English
publishDate 2024-12-01
publisher Firenze University Press
record_format Article
series Phytopathologia Mediterranea
spelling doaj-art-07e11a2046e04170881f5cbb23ea23b12025-08-20T03:08:35ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952024-12-0163336737310.36253/phyto-1556014397Identification of powdery mildew on Prunus rufoides in China, caused by Podosphaera prunigenaZhilong WANG0https://orcid.org/0000-0001-8734-1729Wen LI1https://orcid.org/0000-0002-0983-7910Yueqiu HE2https://orcid.org/0009-0002-8988-9012Feng LIU3Kai FENG4https://orcid.org/0009-0000-4501-7883Liang LIU5https://orcid.org/0000-0001-8044-1006Tao FU6https://orcid.org/0009-0007-4913-6370Qi YE7https://orcid.org/0009-0001-5237-0586Guoliang WANG8https://orcid.org/0009-0004-1767-0817Ningbo City College of Vocational Technology, Ningbo Zhejiang, 315000Ningbo City College of Vocational Technology, Ningbo Zhejiang, 315000Ningbo City College of Vocational Technology, Ningbo Zhejiang, 315000Ningbo City College of Vocational Technology, Ningbo Zhejiang, 315000Ningbo City College of Vocational Technology, Ningbo Zhejiang, 315000Ningbo City College of Vocational Technology, Ningbo Zhejiang, 315000Ningbo City College of Vocational Technology, Ningbo Zhejiang, 315000Ningbo City College of Vocational Technology, Ningbo Zhejiang, 315000College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo Zhejiang, 315000Prunus rufoides is a deciduous wild tree, native to China, which is also used as an ornamental. From late March to December in the years 2018 to 2023, P. rufoides plants growing in Siming Mountain (29°71’08”N, 121°15’12”E), Ningbo, Zhejiang Province, were severely affected by powdery mildew. The disease initially emerged in late March each year, and was characterized as white, irregular mycelial patches on the adaxial surfaces of young leaves. Between July and August, the powdery mildew colonies on affected parts of leaves disappeared, leaving only irregular yellow-brown spots. The disease recurred in September and persisted until late December. Chasmothecia containing asci and ascospores were observed on the leaves in December. Morphological analyses of the chasmothecia indicated a Podosphaera sp. as the causative agent. Molecular identification, based on the internal transcribed spacer (ITS) region (primers ITS4/ITS5) confirmed the identity of the pathogen as Podosphaera prunigena. Koch’s postulates were fulfilled by inoculation tests in which the same pathogen was identified from the inoculated leaf tissue. This study represents the first report confirming the powdery mildew disease on P. rufoides in China to be caused by P. prunigena.https://oajournals.fupress.net/index.php/pm/article/view/15560 erysiphaceaemorphologymolecular analysistaxonomy
spellingShingle Zhilong WANG
Wen LI
Yueqiu HE
Feng LIU
Kai FENG
Liang LIU
Tao FU
Qi YE
Guoliang WANG
Identification of powdery mildew on Prunus rufoides in China, caused by Podosphaera prunigena
Phytopathologia Mediterranea
erysiphaceae
morphology
molecular analysis
taxonomy
title Identification of powdery mildew on Prunus rufoides in China, caused by Podosphaera prunigena
title_full Identification of powdery mildew on Prunus rufoides in China, caused by Podosphaera prunigena
title_fullStr Identification of powdery mildew on Prunus rufoides in China, caused by Podosphaera prunigena
title_full_unstemmed Identification of powdery mildew on Prunus rufoides in China, caused by Podosphaera prunigena
title_short Identification of powdery mildew on Prunus rufoides in China, caused by Podosphaera prunigena
title_sort identification of powdery mildew on prunus rufoides in china caused by podosphaera prunigena
topic erysiphaceae
morphology
molecular analysis
taxonomy
url https://oajournals.fupress.net/index.php/pm/article/view/15560
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