Genomic analyses reveal broad impact of miR-137 on genes associated with malignant transformation and neuronal differentiation in glioblastoma cells.
miR-137 plays critical roles in the nervous system and tumor development; an increase in its expression is required for neuronal differentiation while its reduction is implicated in gliomagenesis. To evaluate the potential of miR-137 in glioblastoma therapy, we conducted genome-wide target mapping i...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0085591 |
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| author | Saleh Tamim Dat T Vo Philip J Uren Mei Qiao Eckart Bindewald Wojciech K Kasprzak Bruce A Shapiro Helder I Nakaya Suzanne C Burns Patricia R Araujo Ichiro Nakano Agnes J Radek Scott Kuersten Andrew D Smith Luiz O F Penalva |
| author_facet | Saleh Tamim Dat T Vo Philip J Uren Mei Qiao Eckart Bindewald Wojciech K Kasprzak Bruce A Shapiro Helder I Nakaya Suzanne C Burns Patricia R Araujo Ichiro Nakano Agnes J Radek Scott Kuersten Andrew D Smith Luiz O F Penalva |
| author_sort | Saleh Tamim |
| collection | DOAJ |
| description | miR-137 plays critical roles in the nervous system and tumor development; an increase in its expression is required for neuronal differentiation while its reduction is implicated in gliomagenesis. To evaluate the potential of miR-137 in glioblastoma therapy, we conducted genome-wide target mapping in glioblastoma cells by measuring the level of association between PABP and mRNAs in cells transfected with miR-137 mimics vs. controls via RIPSeq. Impact on mRNA levels was also measured by RNASeq. By combining the results of both experimental approaches, 1468 genes were found to be negatively impacted by miR-137--among them, 595 (40%) contain miR-137 predicted sites. The most relevant targets include oncogenic proteins and key players in neurogenesis like c-KIT, YBX1, AKT2, CDC42, CDK6 and TGFβ2. Interestingly, we observed that several identified miR-137 targets are also predicted to be regulated by miR-124, miR-128 and miR-7, which are equally implicated in neuronal differentiation and gliomagenesis. We suggest that the concomitant increase of these four miRNAs in neuronal stem cells or their repression in tumor cells could produce a robust regulatory effect with major consequences to neuronal differentiation and tumorigenesis. |
| format | Article |
| id | doaj-art-07dfca16fddd4fbc9d890bfd951dd082 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-07dfca16fddd4fbc9d890bfd951dd0822025-08-20T02:34:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8559110.1371/journal.pone.0085591Genomic analyses reveal broad impact of miR-137 on genes associated with malignant transformation and neuronal differentiation in glioblastoma cells.Saleh TamimDat T VoPhilip J UrenMei QiaoEckart BindewaldWojciech K KasprzakBruce A ShapiroHelder I NakayaSuzanne C BurnsPatricia R AraujoIchiro NakanoAgnes J RadekScott KuerstenAndrew D SmithLuiz O F PenalvamiR-137 plays critical roles in the nervous system and tumor development; an increase in its expression is required for neuronal differentiation while its reduction is implicated in gliomagenesis. To evaluate the potential of miR-137 in glioblastoma therapy, we conducted genome-wide target mapping in glioblastoma cells by measuring the level of association between PABP and mRNAs in cells transfected with miR-137 mimics vs. controls via RIPSeq. Impact on mRNA levels was also measured by RNASeq. By combining the results of both experimental approaches, 1468 genes were found to be negatively impacted by miR-137--among them, 595 (40%) contain miR-137 predicted sites. The most relevant targets include oncogenic proteins and key players in neurogenesis like c-KIT, YBX1, AKT2, CDC42, CDK6 and TGFβ2. Interestingly, we observed that several identified miR-137 targets are also predicted to be regulated by miR-124, miR-128 and miR-7, which are equally implicated in neuronal differentiation and gliomagenesis. We suggest that the concomitant increase of these four miRNAs in neuronal stem cells or their repression in tumor cells could produce a robust regulatory effect with major consequences to neuronal differentiation and tumorigenesis.https://doi.org/10.1371/journal.pone.0085591 |
| spellingShingle | Saleh Tamim Dat T Vo Philip J Uren Mei Qiao Eckart Bindewald Wojciech K Kasprzak Bruce A Shapiro Helder I Nakaya Suzanne C Burns Patricia R Araujo Ichiro Nakano Agnes J Radek Scott Kuersten Andrew D Smith Luiz O F Penalva Genomic analyses reveal broad impact of miR-137 on genes associated with malignant transformation and neuronal differentiation in glioblastoma cells. PLoS ONE |
| title | Genomic analyses reveal broad impact of miR-137 on genes associated with malignant transformation and neuronal differentiation in glioblastoma cells. |
| title_full | Genomic analyses reveal broad impact of miR-137 on genes associated with malignant transformation and neuronal differentiation in glioblastoma cells. |
| title_fullStr | Genomic analyses reveal broad impact of miR-137 on genes associated with malignant transformation and neuronal differentiation in glioblastoma cells. |
| title_full_unstemmed | Genomic analyses reveal broad impact of miR-137 on genes associated with malignant transformation and neuronal differentiation in glioblastoma cells. |
| title_short | Genomic analyses reveal broad impact of miR-137 on genes associated with malignant transformation and neuronal differentiation in glioblastoma cells. |
| title_sort | genomic analyses reveal broad impact of mir 137 on genes associated with malignant transformation and neuronal differentiation in glioblastoma cells |
| url | https://doi.org/10.1371/journal.pone.0085591 |
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