Pigment mediated biogenic synthesis of silver nanoparticles using diatom Amphora sp. and its antimicrobial activity
Light induced biosynthesis of polycrystalline silver nanoparticles (SNPs) using the aqueous extract of a diatom Amphora-46 was studied. Rapid formation of stable SNPs was observed only on exposure of the reaction mixture to light. Strong surface plasmon resonance at 415 nm due to SNP formation was c...
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| Language: | English |
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Springer
2015-11-01
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| Series: | Journal of Saudi Chemical Society |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319610314000891 |
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| author | Jayashree Jena Nilotpala Pradhan Bishnu Prasad Dash Prasanna Kumar Panda Barada Kanta Mishra |
| author_facet | Jayashree Jena Nilotpala Pradhan Bishnu Prasad Dash Prasanna Kumar Panda Barada Kanta Mishra |
| author_sort | Jayashree Jena |
| collection | DOAJ |
| description | Light induced biosynthesis of polycrystalline silver nanoparticles (SNPs) using the aqueous extract of a diatom Amphora-46 was studied. Rapid formation of stable SNPs was observed only on exposure of the reaction mixture to light. Strong surface plasmon resonance at 415 nm due to SNP formation was confirmed by spectroscopic analysis. TEM analysis confirmed the formation of polycrystalline spherical SNPs of an average size of 20–25 nm which was further corroborated by XRD, EDAX and SAED results. Compositional analysis using EDAX showed strong characteristic signal for silver. The XRD spectra show four intense diffraction peaks at 38.48°, 44°, 64.74°, and 77.4° which correspond well to (111), (200), (220), and (311) plane of (fcc) polycrystalline SNP and the intensity of peak at (111) plane is more than the other peaks, suggesting that this plane is the predominant one. Both XRD and SAED results clearly indicated that the SNPs were polycrystalline in nature and were of high purity. The bio-molecule responsible for the reduction of silver ion was identified to be a photosynthetic pigment fucoxanthin, which is light sensitive and acts as a reducing agent. Furthermore, the synthesized SNPs possess significant antimicrobial activity against gram positive and gram negative bacteria.
This study demonstrates for the first time, the involvement of photosynthetic pigment fucoxanthin isolated from Amphora-46 in silver nanoparticle formation through a light dependent reaction. |
| format | Article |
| id | doaj-art-07db7fcc147f4b699438ed38c1f6bcdb |
| institution | Kabale University |
| issn | 1319-6103 |
| language | English |
| publishDate | 2015-11-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Saudi Chemical Society |
| spelling | doaj-art-07db7fcc147f4b699438ed38c1f6bcdb2025-08-20T03:55:28ZengSpringerJournal of Saudi Chemical Society1319-61032015-11-0119666166610.1016/j.jscs.2014.06.005Pigment mediated biogenic synthesis of silver nanoparticles using diatom Amphora sp. and its antimicrobial activityJayashree Jena0Nilotpala Pradhan1Bishnu Prasad Dash2Prasanna Kumar Panda3Barada Kanta Mishra4Bioresources Engineering Department, CSIR-Institute of Minerals and Materials Technology, Bhubaneswar 751013, Odisha, IndiaBioresources Engineering Department, CSIR-Institute of Minerals and Materials Technology, Bhubaneswar 751013, Odisha, IndiaFakir Mohan University, Balasore, Odisha, IndiaBioresources Engineering Department, CSIR-Institute of Minerals and Materials Technology, Bhubaneswar 751013, Odisha, IndiaCSIR-Institute of Minerals and Materials Technology, Bhubaneswar 751013, Odisha, IndiaLight induced biosynthesis of polycrystalline silver nanoparticles (SNPs) using the aqueous extract of a diatom Amphora-46 was studied. Rapid formation of stable SNPs was observed only on exposure of the reaction mixture to light. Strong surface plasmon resonance at 415 nm due to SNP formation was confirmed by spectroscopic analysis. TEM analysis confirmed the formation of polycrystalline spherical SNPs of an average size of 20–25 nm which was further corroborated by XRD, EDAX and SAED results. Compositional analysis using EDAX showed strong characteristic signal for silver. The XRD spectra show four intense diffraction peaks at 38.48°, 44°, 64.74°, and 77.4° which correspond well to (111), (200), (220), and (311) plane of (fcc) polycrystalline SNP and the intensity of peak at (111) plane is more than the other peaks, suggesting that this plane is the predominant one. Both XRD and SAED results clearly indicated that the SNPs were polycrystalline in nature and were of high purity. The bio-molecule responsible for the reduction of silver ion was identified to be a photosynthetic pigment fucoxanthin, which is light sensitive and acts as a reducing agent. Furthermore, the synthesized SNPs possess significant antimicrobial activity against gram positive and gram negative bacteria. This study demonstrates for the first time, the involvement of photosynthetic pigment fucoxanthin isolated from Amphora-46 in silver nanoparticle formation through a light dependent reaction.http://www.sciencedirect.com/science/article/pii/S1319610314000891Silver nanoparticlesDiatomAmphoraFucoxanthinAntimicrobial activity |
| spellingShingle | Jayashree Jena Nilotpala Pradhan Bishnu Prasad Dash Prasanna Kumar Panda Barada Kanta Mishra Pigment mediated biogenic synthesis of silver nanoparticles using diatom Amphora sp. and its antimicrobial activity Journal of Saudi Chemical Society Silver nanoparticles Diatom Amphora Fucoxanthin Antimicrobial activity |
| title | Pigment mediated biogenic synthesis of silver nanoparticles using diatom Amphora sp. and its antimicrobial activity |
| title_full | Pigment mediated biogenic synthesis of silver nanoparticles using diatom Amphora sp. and its antimicrobial activity |
| title_fullStr | Pigment mediated biogenic synthesis of silver nanoparticles using diatom Amphora sp. and its antimicrobial activity |
| title_full_unstemmed | Pigment mediated biogenic synthesis of silver nanoparticles using diatom Amphora sp. and its antimicrobial activity |
| title_short | Pigment mediated biogenic synthesis of silver nanoparticles using diatom Amphora sp. and its antimicrobial activity |
| title_sort | pigment mediated biogenic synthesis of silver nanoparticles using diatom amphora sp and its antimicrobial activity |
| topic | Silver nanoparticles Diatom Amphora Fucoxanthin Antimicrobial activity |
| url | http://www.sciencedirect.com/science/article/pii/S1319610314000891 |
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