Proteomic profiling of saliva reveals association of complement system with primary Sjögren's syndrome
Abstract Introduction To compare the saliva proteomes of experimental Sjögren's syndrome (ESS) model mice and healthy controls to identify potential diagnostic biomarkers for primary Sjögren's syndrome (pSS). Methods Proteins were extracted from the saliva of three ESS and three normal con...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-12-01
|
| Series: | Immunity, Inflammation and Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/iid3.529 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850074435668672512 |
|---|---|
| author | Mingde Li Yajun Qi Guizhen Wang Su Bu Ming Chen Jiahui Yu Tianyang Luo Lulu Meng Anran Dai Yong Zhou Shuai Liu Xingxing Huo |
| author_facet | Mingde Li Yajun Qi Guizhen Wang Su Bu Ming Chen Jiahui Yu Tianyang Luo Lulu Meng Anran Dai Yong Zhou Shuai Liu Xingxing Huo |
| author_sort | Mingde Li |
| collection | DOAJ |
| description | Abstract Introduction To compare the saliva proteomes of experimental Sjögren's syndrome (ESS) model mice and healthy controls to identify potential diagnostic biomarkers for primary Sjögren's syndrome (pSS). Methods Proteins were extracted from the saliva of three ESS and three normal control mice using the data‐independent acquisition technique. R language was used to identify the differentially expressed proteins (DEPs). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed to functionally annotate the DEPs. The protein–protein interaction (PPI) network was constructed and the core proteins were identified with the STRING website and Cytoscape software. The concentrations of Serpin family G member 1 (SERPING1), C3, complement factor H (CFH), fibrinogen alpha (FGA), and fibrinogen gamma (FGG) in saliva were determined by ELISA. Results A total of 1722 DEPs were identified in the saliva of the ESS mice relative to the controls, of which 50 showed significantly different expression levels between the two groups. SERPING1, C3, CFH, FGA, and FGG were significantly downregulated, and keratin 4 (Krt4) and transglutaminase 3 (TGM3) were upregulated in the saliva of ESS mice. The PPI network showed that SERPING1, C3, FGG, FGA, TGM3, and hemopexin (HPX) were the core proteins. ELISA results showed that the expression of C3, CFH, FGA, and SERPING1 were significantly downregulated in the saliva of ESS mice. However, the expression of FGG was a little downregulated but with no significant difference. SERPING1, FGG, and FGA may downregulate the complement C3 by inhibiting immune complement system, thereby promoting pSS progression. Conclusions The salivary proteome of ESS mice was markedly different from that of healthy controls, suggesting that salivary proteomics is a promising noninvasive diagnostic tool for pSS. SERPING1, C3, CFH, FGA, and FGG are potential biomarkers of pSS. |
| format | Article |
| id | doaj-art-07d0283d102244ef9846d8676a9ffccb |
| institution | DOAJ |
| issn | 2050-4527 |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj-art-07d0283d102244ef9846d8676a9ffccb2025-08-20T02:46:35ZengWileyImmunity, Inflammation and Disease2050-45272021-12-01941724173910.1002/iid3.529Proteomic profiling of saliva reveals association of complement system with primary Sjögren's syndromeMingde Li0Yajun Qi1Guizhen Wang2Su Bu3Ming Chen4Jiahui Yu5Tianyang Luo6Lulu Meng7Anran Dai8Yong Zhou9Shuai Liu10Xingxing Huo11Experimental Center of Clinical Research, Scientific Research Department The First Affiliated Hospital of Anhui University of Chinese Medicine Hefei Anhui ChinaDepartment of Traditional Chinese Medicine, College of Acupuncture and Massage Anhui University of traditional Chinese Medicine Hefei Anhui ChinaDepartment of Rheumatology The First Affiliated Hospital of Anhui University of Chinese Medicine Hefei ChinaExperimental Center of Clinical Research, Scientific Research Department The First Affiliated Hospital of Anhui University of Chinese Medicine Hefei Anhui ChinaDepartment of Anesthesiology, Second Clinical Medical College Anhui Medical University Hefei Anhui ChinaDepartment of Traditional Chinese Pharmacology, College of Pharmacy Anhui Medical University Hefei Anhui ChinaDepartment of Anesthesiology, Second Clinical Medical College Anhui Medical University Hefei Anhui ChinaExperimental Center of Clinical Research, Scientific Research Department The First Affiliated Hospital of Anhui University of Chinese Medicine Hefei Anhui ChinaExperimental Center of Clinical Research, Scientific Research Department The First Affiliated Hospital of Anhui University of Chinese Medicine Hefei Anhui ChinaExperimental Center of Clinical Research, Scientific Research Department The First Affiliated Hospital of Anhui University of Chinese Medicine Hefei Anhui ChinaExperimental Center of Clinical Research, Scientific Research Department The First Affiliated Hospital of Anhui University of Chinese Medicine Hefei Anhui ChinaExperimental Center of Clinical Research, Scientific Research Department The First Affiliated Hospital of Anhui University of Chinese Medicine Hefei Anhui ChinaAbstract Introduction To compare the saliva proteomes of experimental Sjögren's syndrome (ESS) model mice and healthy controls to identify potential diagnostic biomarkers for primary Sjögren's syndrome (pSS). Methods Proteins were extracted from the saliva of three ESS and three normal control mice using the data‐independent acquisition technique. R language was used to identify the differentially expressed proteins (DEPs). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed to functionally annotate the DEPs. The protein–protein interaction (PPI) network was constructed and the core proteins were identified with the STRING website and Cytoscape software. The concentrations of Serpin family G member 1 (SERPING1), C3, complement factor H (CFH), fibrinogen alpha (FGA), and fibrinogen gamma (FGG) in saliva were determined by ELISA. Results A total of 1722 DEPs were identified in the saliva of the ESS mice relative to the controls, of which 50 showed significantly different expression levels between the two groups. SERPING1, C3, CFH, FGA, and FGG were significantly downregulated, and keratin 4 (Krt4) and transglutaminase 3 (TGM3) were upregulated in the saliva of ESS mice. The PPI network showed that SERPING1, C3, FGG, FGA, TGM3, and hemopexin (HPX) were the core proteins. ELISA results showed that the expression of C3, CFH, FGA, and SERPING1 were significantly downregulated in the saliva of ESS mice. However, the expression of FGG was a little downregulated but with no significant difference. SERPING1, FGG, and FGA may downregulate the complement C3 by inhibiting immune complement system, thereby promoting pSS progression. Conclusions The salivary proteome of ESS mice was markedly different from that of healthy controls, suggesting that salivary proteomics is a promising noninvasive diagnostic tool for pSS. SERPING1, C3, CFH, FGA, and FGG are potential biomarkers of pSS.https://doi.org/10.1002/iid3.529biomarkersdifferentially expressed proteinsprimary Sjögren's syndromesalivary proteomic |
| spellingShingle | Mingde Li Yajun Qi Guizhen Wang Su Bu Ming Chen Jiahui Yu Tianyang Luo Lulu Meng Anran Dai Yong Zhou Shuai Liu Xingxing Huo Proteomic profiling of saliva reveals association of complement system with primary Sjögren's syndrome Immunity, Inflammation and Disease biomarkers differentially expressed proteins primary Sjögren's syndrome salivary proteomic |
| title | Proteomic profiling of saliva reveals association of complement system with primary Sjögren's syndrome |
| title_full | Proteomic profiling of saliva reveals association of complement system with primary Sjögren's syndrome |
| title_fullStr | Proteomic profiling of saliva reveals association of complement system with primary Sjögren's syndrome |
| title_full_unstemmed | Proteomic profiling of saliva reveals association of complement system with primary Sjögren's syndrome |
| title_short | Proteomic profiling of saliva reveals association of complement system with primary Sjögren's syndrome |
| title_sort | proteomic profiling of saliva reveals association of complement system with primary sjogren s syndrome |
| topic | biomarkers differentially expressed proteins primary Sjögren's syndrome salivary proteomic |
| url | https://doi.org/10.1002/iid3.529 |
| work_keys_str_mv | AT mingdeli proteomicprofilingofsalivarevealsassociationofcomplementsystemwithprimarysjogrenssyndrome AT yajunqi proteomicprofilingofsalivarevealsassociationofcomplementsystemwithprimarysjogrenssyndrome AT guizhenwang proteomicprofilingofsalivarevealsassociationofcomplementsystemwithprimarysjogrenssyndrome AT subu proteomicprofilingofsalivarevealsassociationofcomplementsystemwithprimarysjogrenssyndrome AT mingchen proteomicprofilingofsalivarevealsassociationofcomplementsystemwithprimarysjogrenssyndrome AT jiahuiyu proteomicprofilingofsalivarevealsassociationofcomplementsystemwithprimarysjogrenssyndrome AT tianyangluo proteomicprofilingofsalivarevealsassociationofcomplementsystemwithprimarysjogrenssyndrome AT lulumeng proteomicprofilingofsalivarevealsassociationofcomplementsystemwithprimarysjogrenssyndrome AT anrandai proteomicprofilingofsalivarevealsassociationofcomplementsystemwithprimarysjogrenssyndrome AT yongzhou proteomicprofilingofsalivarevealsassociationofcomplementsystemwithprimarysjogrenssyndrome AT shuailiu proteomicprofilingofsalivarevealsassociationofcomplementsystemwithprimarysjogrenssyndrome AT xingxinghuo proteomicprofilingofsalivarevealsassociationofcomplementsystemwithprimarysjogrenssyndrome |