A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies.
The development of high-yielding crops with drought tolerance is necessary to increase food, feed, fiber and fuel production. Methods that create similar environmental conditions for a large number of genotypes are essential to investigate plant responses to drought in gene discovery studies. Modern...
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| _version_ | 1850076445106241536 |
|---|---|
| author | Diego Ortiz Alexander G Litvin Maria G Salas Fernandez |
| author_facet | Diego Ortiz Alexander G Litvin Maria G Salas Fernandez |
| author_sort | Diego Ortiz |
| collection | DOAJ |
| description | The development of high-yielding crops with drought tolerance is necessary to increase food, feed, fiber and fuel production. Methods that create similar environmental conditions for a large number of genotypes are essential to investigate plant responses to drought in gene discovery studies. Modern facilities that control water availability for each plant remain cost-prohibited to some sections of the research community. We present an alternative cost-effective automated irrigation system scalable for a high-throughput and controlled dry-down treatment of plants. This system was tested in sorghum using two experiments. First, four genotypes were subjected to ten days of dry-down to achieve three final Volumetric Water Content (VWC) levels: drought (0.10 and 0.20 m3 m-3) and control (0.30 m3 m-3). The final average VWC was 0.11, 0.22, and 0.31 m3 m-3, respectively, and significant differences in biomass accumulation were observed between control and drought treatments. Second, 42 diverse sorghum genotypes were subjected to a seven-day dry-down treatment for a final drought stress of 0.15 m3 m-3 VWC. The final average VWC was 0.17 m3 m-3, and plants presented significant differences in photosynthetic rate during the drought period. These results demonstrate that cost-effective automation systems can successfully control substrate water content for each plant, to accurately compare their phenotypic responses to drought, and be scaled up for high-throughput phenotyping studies. |
| format | Article |
| id | doaj-art-07ce163e982a41b4959679cb0aad462f |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-07ce163e982a41b4959679cb0aad462f2025-08-20T02:46:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01136e019854610.1371/journal.pone.0198546A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies.Diego OrtizAlexander G LitvinMaria G Salas FernandezThe development of high-yielding crops with drought tolerance is necessary to increase food, feed, fiber and fuel production. Methods that create similar environmental conditions for a large number of genotypes are essential to investigate plant responses to drought in gene discovery studies. Modern facilities that control water availability for each plant remain cost-prohibited to some sections of the research community. We present an alternative cost-effective automated irrigation system scalable for a high-throughput and controlled dry-down treatment of plants. This system was tested in sorghum using two experiments. First, four genotypes were subjected to ten days of dry-down to achieve three final Volumetric Water Content (VWC) levels: drought (0.10 and 0.20 m3 m-3) and control (0.30 m3 m-3). The final average VWC was 0.11, 0.22, and 0.31 m3 m-3, respectively, and significant differences in biomass accumulation were observed between control and drought treatments. Second, 42 diverse sorghum genotypes were subjected to a seven-day dry-down treatment for a final drought stress of 0.15 m3 m-3 VWC. The final average VWC was 0.17 m3 m-3, and plants presented significant differences in photosynthetic rate during the drought period. These results demonstrate that cost-effective automation systems can successfully control substrate water content for each plant, to accurately compare their phenotypic responses to drought, and be scaled up for high-throughput phenotyping studies.https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0198546/1/pone.0198546.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210222%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210222T202325Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=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 |
| spellingShingle | Diego Ortiz Alexander G Litvin Maria G Salas Fernandez A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies. PLoS ONE |
| title | A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies. |
| title_full | A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies. |
| title_fullStr | A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies. |
| title_full_unstemmed | A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies. |
| title_short | A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies. |
| title_sort | cost effective and customizable automated irrigation system for precise high throughput phenotyping in drought stress studies |
| url | https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0198546/1/pone.0198546.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210222%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210222T202325Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=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 |
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