Impact of Phosphogypsum on Viability of <i>Trichuris suis</i> Eggs in Anaerobic Digestion of Swine Manure

Waste from livestock farms contains various pathogens, including eggs and larvae of helminths—pathogens of parasitic diseases harmful to animals and humans. One of the methods for their effective processing to obtain biofertilizer and biofuel is anaerobic digestion, which requires further improvemen...

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Main Authors: Olexandra Boyko, Viktor Brygadyrenko, Yelizaveta Chernysh, Viktoriia Chubur, Hynek Roubík
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/5/1165
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author Olexandra Boyko
Viktor Brygadyrenko
Yelizaveta Chernysh
Viktoriia Chubur
Hynek Roubík
author_facet Olexandra Boyko
Viktor Brygadyrenko
Yelizaveta Chernysh
Viktoriia Chubur
Hynek Roubík
author_sort Olexandra Boyko
collection DOAJ
description Waste from livestock farms contains various pathogens, including eggs and larvae of helminths—pathogens of parasitic diseases harmful to animals and humans. One of the methods for their effective processing to obtain biofertilizer and biofuel is anaerobic digestion, which requires further improvement to completely suppress the viability of pathogenic microorganisms in mesophilic conditions. To this end, the use of anaerobic digestion under sulfate reduction conditions to suppress pathogens using biogenic hydrogen sulfide is promising. Consequently, this study aims to study the effect of a sulfur-containing additive such as phosphogypsum on the disinfection of pig manure during anaerobic digestion. Egg mortality was already found to increase significantly compared to the control (80% and more), even at a minimum concentration of phosphogypsum (5%), on the fifth day of the experiment. At the same time, the maximum effect (100% mortality of <i>Trichuris suis</i> eggs) was recorded at a 10% concentration of phosphogypsum, starting from the 10th day of the study. Our experiment showed that changes in anaerobic digestion conditions using phosphogypsum could positively affect digestate disinfection. However, further research is needed to optimize the conditions of the process for an effective combination of disinfection with the production of environmentally safe organic fertilizers and high-quality biogas with a high level of methane.
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spelling doaj-art-07c7c8d4aff94068ba83bd3e66682a412025-08-20T01:56:41ZengMDPI AGMicroorganisms2076-26072025-05-01135116510.3390/microorganisms13051165Impact of Phosphogypsum on Viability of <i>Trichuris suis</i> Eggs in Anaerobic Digestion of Swine ManureOlexandra Boyko0Viktor Brygadyrenko1Yelizaveta Chernysh2Viktoriia Chubur3Hynek Roubík4Department of Parasitology and Veterinary and Sanitary Expertise, Dnipro State Agrarian and Economic University, Serhii Efremov St., 25, 49000 Dnipro, UkraineDepartment of Biodiversity and Ecology, Oles Honchar Dnipro National University, Nauky Av., 72, 49010 Dnipro, UkraineDepartment of Sustainable Technologies, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamýcká 129, 16500 Prague, Czech RepublicDepartment of Sustainable Technologies, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamýcká 129, 16500 Prague, Czech RepublicDepartment of Sustainable Technologies, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamýcká 129, 16500 Prague, Czech RepublicWaste from livestock farms contains various pathogens, including eggs and larvae of helminths—pathogens of parasitic diseases harmful to animals and humans. One of the methods for their effective processing to obtain biofertilizer and biofuel is anaerobic digestion, which requires further improvement to completely suppress the viability of pathogenic microorganisms in mesophilic conditions. To this end, the use of anaerobic digestion under sulfate reduction conditions to suppress pathogens using biogenic hydrogen sulfide is promising. Consequently, this study aims to study the effect of a sulfur-containing additive such as phosphogypsum on the disinfection of pig manure during anaerobic digestion. Egg mortality was already found to increase significantly compared to the control (80% and more), even at a minimum concentration of phosphogypsum (5%), on the fifth day of the experiment. At the same time, the maximum effect (100% mortality of <i>Trichuris suis</i> eggs) was recorded at a 10% concentration of phosphogypsum, starting from the 10th day of the study. Our experiment showed that changes in anaerobic digestion conditions using phosphogypsum could positively affect digestate disinfection. However, further research is needed to optimize the conditions of the process for an effective combination of disinfection with the production of environmentally safe organic fertilizers and high-quality biogas with a high level of methane.https://www.mdpi.com/2076-2607/13/5/1165phosphogypsumanaerobic digestionsulfate reductiondigestion<i>Trichuris suis</i>Nematoda
spellingShingle Olexandra Boyko
Viktor Brygadyrenko
Yelizaveta Chernysh
Viktoriia Chubur
Hynek Roubík
Impact of Phosphogypsum on Viability of <i>Trichuris suis</i> Eggs in Anaerobic Digestion of Swine Manure
Microorganisms
phosphogypsum
anaerobic digestion
sulfate reduction
digestion
<i>Trichuris suis</i>
Nematoda
title Impact of Phosphogypsum on Viability of <i>Trichuris suis</i> Eggs in Anaerobic Digestion of Swine Manure
title_full Impact of Phosphogypsum on Viability of <i>Trichuris suis</i> Eggs in Anaerobic Digestion of Swine Manure
title_fullStr Impact of Phosphogypsum on Viability of <i>Trichuris suis</i> Eggs in Anaerobic Digestion of Swine Manure
title_full_unstemmed Impact of Phosphogypsum on Viability of <i>Trichuris suis</i> Eggs in Anaerobic Digestion of Swine Manure
title_short Impact of Phosphogypsum on Viability of <i>Trichuris suis</i> Eggs in Anaerobic Digestion of Swine Manure
title_sort impact of phosphogypsum on viability of i trichuris suis i eggs in anaerobic digestion of swine manure
topic phosphogypsum
anaerobic digestion
sulfate reduction
digestion
<i>Trichuris suis</i>
Nematoda
url https://www.mdpi.com/2076-2607/13/5/1165
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