STUDY OF ANTIBIOTIC RESISTANCE PATTERN OF CLINICAL ISOLATES
The antibiotic resistance pattern of the forty five clinical isolates (consisting of 33 Gram negative and 12 Gam positive bacteria) were determined, against nine antibiotics including streptomycin, gentamycin, neomycin, tetracycline, kanamycin, ampicillin, amoxicillin, chloramphenicol and vibramyci...
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| Language: | English |
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Baqai University Press
2024-01-01
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| Series: | Baqai Journal of Health Sciences |
| Online Access: | https://journals.baqai.edu.pk/index.php/CS/article/view/7 |
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The antibiotic resistance pattern of the forty five clinical isolates (consisting of 33 Gram negative and 12 Gam positive bacteria) were determined, against nine antibiotics including streptomycin, gentamycin, neomycin, tetracycline, kanamycin, ampicillin, amoxicillin, chloramphenicol and vibramycin.
The level of resistance was determined by Replica plate technique and tube dilution method. Minimum Inhibitory Concentration (MIC), MIC50, MIC90 and % of sensitive and resistant organisms were determined. The Gram negative isolates were found resistant to many antibiotics as compared to the Gram positive clinical isolates. Vibramycin was the only antibiotics which was found effective against 85% of the Gram negative and almost all the Gram positive clinical isolates tested. Whereas against the rest of the antibiotics moderate to higher resistance were observed.
The resistance of the clinical isolates was due to the presence of antibiotic resistant plasmid. This was determined by agarose gel electrophoresis. It was found that 75% of the Gram negative and 58% Gram positive clinical isolates have plasmid and probably the higher resistance pattern was due to presence of R-plasmid.
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| format | Article |
| id | doaj-art-07c303f480f2405f9f117f877aa01f6d |
| institution | Kabale University |
| issn | 2312-4423 2312-6884 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Baqai University Press |
| record_format | Article |
| series | Baqai Journal of Health Sciences |
| spelling | doaj-art-07c303f480f2405f9f117f877aa01f6d2025-02-11T18:46:00ZengBaqai University PressBaqai Journal of Health Sciences2312-44232312-68842024-01-0111STUDY OF ANTIBIOTIC RESISTANCE PATTERN OF CLINICAL ISOLATES The antibiotic resistance pattern of the forty five clinical isolates (consisting of 33 Gram negative and 12 Gam positive bacteria) were determined, against nine antibiotics including streptomycin, gentamycin, neomycin, tetracycline, kanamycin, ampicillin, amoxicillin, chloramphenicol and vibramycin. The level of resistance was determined by Replica plate technique and tube dilution method. Minimum Inhibitory Concentration (MIC), MIC50, MIC90 and % of sensitive and resistant organisms were determined. The Gram negative isolates were found resistant to many antibiotics as compared to the Gram positive clinical isolates. Vibramycin was the only antibiotics which was found effective against 85% of the Gram negative and almost all the Gram positive clinical isolates tested. Whereas against the rest of the antibiotics moderate to higher resistance were observed. The resistance of the clinical isolates was due to the presence of antibiotic resistant plasmid. This was determined by agarose gel electrophoresis. It was found that 75% of the Gram negative and 58% Gram positive clinical isolates have plasmid and probably the higher resistance pattern was due to presence of R-plasmid. https://journals.baqai.edu.pk/index.php/CS/article/view/7 |
| spellingShingle | STUDY OF ANTIBIOTIC RESISTANCE PATTERN OF CLINICAL ISOLATES Baqai Journal of Health Sciences |
| title | STUDY OF ANTIBIOTIC RESISTANCE PATTERN OF CLINICAL ISOLATES |
| title_full | STUDY OF ANTIBIOTIC RESISTANCE PATTERN OF CLINICAL ISOLATES |
| title_fullStr | STUDY OF ANTIBIOTIC RESISTANCE PATTERN OF CLINICAL ISOLATES |
| title_full_unstemmed | STUDY OF ANTIBIOTIC RESISTANCE PATTERN OF CLINICAL ISOLATES |
| title_short | STUDY OF ANTIBIOTIC RESISTANCE PATTERN OF CLINICAL ISOLATES |
| title_sort | study of antibiotic resistance pattern of clinical isolates |
| url | https://journals.baqai.edu.pk/index.php/CS/article/view/7 |