A critical review on purification methods and applications of marine algal haloperoxidases
Marine algae are a rich and underexplored source of haloperoxidases, enzymes with wide ranging applications in biocatalysis, pharmaceuticals, and environmental bioremediation due to their ability to catalyze the halogenation of organic compounds. This review focuses on the recent advancements in the...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Natural Products |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fntpr.2025.1537097/full |
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| author | Abdellatif Rahim Rania Benjamaa Abdel Khalid Essamadi Abdelbasset Chafik |
| author_facet | Abdellatif Rahim Rania Benjamaa Abdel Khalid Essamadi Abdelbasset Chafik |
| author_sort | Abdellatif Rahim |
| collection | DOAJ |
| description | Marine algae are a rich and underexplored source of haloperoxidases, enzymes with wide ranging applications in biocatalysis, pharmaceuticals, and environmental bioremediation due to their ability to catalyze the halogenation of organic compounds. This review focuses on the recent advancements in the purification of haloperoxidases from marine algae, highlighting both traditional and innovative methods. We discussed the limited exploration of green algal haloperoxidases, and the potential for discovering novel enzymes with unique properties. The review examines the advantages and disadvantages of chromatographic techniques, such as ion-exchange, size exclusion, and affinity chromatography, and explores emerging alternatives, including aqueous two-phase systems (ATPS) and microfluidic systems, for improving enzyme yield, purity, and stability. The use of ATPS to address challenges posed by alginate-rich brown algae is emphasized, along with optimization strategies for scaling up purification processes. The growing importance of sustainable and green chemistry approaches to minimize environmental impact while achieving high purification efficiency is also discussed. By analyzing current purification techniques, identifying knowledge gaps, and suggesting future research directions, this review aims to provide meaningful insights into the purification and industrial applications of haloperoxidases, thereby stimulating further exploration and innovation in this field. |
| format | Article |
| id | doaj-art-07bebb0748ca40c7983bd0e7e7e1c0a2 |
| institution | DOAJ |
| issn | 2813-2602 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Natural Products |
| spelling | doaj-art-07bebb0748ca40c7983bd0e7e7e1c0a22025-08-20T02:52:45ZengFrontiers Media S.A.Frontiers in Natural Products2813-26022025-03-01410.3389/fntpr.2025.15370971537097A critical review on purification methods and applications of marine algal haloperoxidasesAbdellatif Rahim0Rania Benjamaa1Abdel Khalid Essamadi2Abdelbasset Chafik3Laboratory of Biochemistry, Neurosciences, Natural Resources and Environment, Hassan First University, Faculty of Sciences and Technology, Settat, MoroccoLaboratory of Biochemistry, Neurosciences, Natural Resources and Environment, Hassan First University, Faculty of Sciences and Technology, Settat, MoroccoLaboratory of Biochemistry, Neurosciences, Natural Resources and Environment, Hassan First University, Faculty of Sciences and Technology, Settat, MoroccoHigher School of Technology of El Kelâa des Sraghna, Cadi Ayyad University, El Kelâa des Sraghna, MoroccoMarine algae are a rich and underexplored source of haloperoxidases, enzymes with wide ranging applications in biocatalysis, pharmaceuticals, and environmental bioremediation due to their ability to catalyze the halogenation of organic compounds. This review focuses on the recent advancements in the purification of haloperoxidases from marine algae, highlighting both traditional and innovative methods. We discussed the limited exploration of green algal haloperoxidases, and the potential for discovering novel enzymes with unique properties. The review examines the advantages and disadvantages of chromatographic techniques, such as ion-exchange, size exclusion, and affinity chromatography, and explores emerging alternatives, including aqueous two-phase systems (ATPS) and microfluidic systems, for improving enzyme yield, purity, and stability. The use of ATPS to address challenges posed by alginate-rich brown algae is emphasized, along with optimization strategies for scaling up purification processes. The growing importance of sustainable and green chemistry approaches to minimize environmental impact while achieving high purification efficiency is also discussed. By analyzing current purification techniques, identifying knowledge gaps, and suggesting future research directions, this review aims to provide meaningful insights into the purification and industrial applications of haloperoxidases, thereby stimulating further exploration and innovation in this field.https://www.frontiersin.org/articles/10.3389/fntpr.2025.1537097/fullmarine algaehaloperoxidasespurification techniquesaqueous two-phase systemsindustrial applicationsbiocatalysis |
| spellingShingle | Abdellatif Rahim Rania Benjamaa Abdel Khalid Essamadi Abdelbasset Chafik A critical review on purification methods and applications of marine algal haloperoxidases Frontiers in Natural Products marine algae haloperoxidases purification techniques aqueous two-phase systems industrial applications biocatalysis |
| title | A critical review on purification methods and applications of marine algal haloperoxidases |
| title_full | A critical review on purification methods and applications of marine algal haloperoxidases |
| title_fullStr | A critical review on purification methods and applications of marine algal haloperoxidases |
| title_full_unstemmed | A critical review on purification methods and applications of marine algal haloperoxidases |
| title_short | A critical review on purification methods and applications of marine algal haloperoxidases |
| title_sort | critical review on purification methods and applications of marine algal haloperoxidases |
| topic | marine algae haloperoxidases purification techniques aqueous two-phase systems industrial applications biocatalysis |
| url | https://www.frontiersin.org/articles/10.3389/fntpr.2025.1537097/full |
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