RNA-binding protein DAZAP1 accelerates the advancement of pancreatic cancer by inhibiting ferroptosis
Abstract Background Pancreatic cancer (PC) is a highly aggressive malignancy with a poor prognosis due to its late-stage diagnosis and limited treatment options. Objectives This study aimed to elucidate the molecular mechanisms underlying PC progression and identify potential molecular targets for i...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40001-024-02261-0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559782878609408 |
|---|---|
| author | Xinqing Wang Hao Fan Xiaoping Ye Yu Hu Yan Xiao Ming Zhang Yonghui Xu Jianjun Song Yongyun Luo |
| author_facet | Xinqing Wang Hao Fan Xiaoping Ye Yu Hu Yan Xiao Ming Zhang Yonghui Xu Jianjun Song Yongyun Luo |
| author_sort | Xinqing Wang |
| collection | DOAJ |
| description | Abstract Background Pancreatic cancer (PC) is a highly aggressive malignancy with a poor prognosis due to its late-stage diagnosis and limited treatment options. Objectives This study aimed to elucidate the molecular mechanisms underlying PC progression and identify potential molecular targets for its diagnosis and treatment. Methods DAZAP1 expression in PC tissues, normal tissues and cell lines was assessed using immunohistochemistry (IHC), reverse transcription–quantitative polymerase chain reaction (RT–qPCR) and western blotting. DAZAP1 knockdown was achieved through plasmid transfection, and its effects on ferroptosis and PC progression were evaluated using RT–qPCR, western blotting, CCK-8 assays, EdU staining, Fe2+ content measurement, reactive oxygen species (ROS) detection, wound healing and Transwell migration assays. Results DAZAP1 expression was significantly upregulated in PC tissues and cell lines compared to normal counterparts. DAZAP1 knockdown suppressed PC cell proliferation and induced ferroptosis, while ferroptosis inhibition reversed these effects, enhancing PC cell proliferation and metastasis. Conclusions DAZAP1 suppression promotes ferroptosis, thereby inhibiting PC cell proliferation and metastasis. These findings suggest that DAZAP1 is a potential therapeutic target for PC. |
| format | Article |
| id | doaj-art-07b1f72ab967494f9270c0499f1d9f40 |
| institution | Kabale University |
| issn | 2047-783X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj-art-07b1f72ab967494f9270c0499f1d9f402025-01-05T12:12:12ZengBMCEuropean Journal of Medical Research2047-783X2025-01-0130111010.1186/s40001-024-02261-0RNA-binding protein DAZAP1 accelerates the advancement of pancreatic cancer by inhibiting ferroptosisXinqing Wang0Hao Fan1Xiaoping Ye2Yu Hu3Yan Xiao4Ming Zhang5Yonghui Xu6Jianjun Song7Yongyun Luo8Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical UniversitySchool of Clinical Medicine, Ningxia Medical UniversityDepartment of Hepatobiliary Surgery, General Hospital of Ningxia Medical UniversitySchool of Clinical Medicine, Ningxia Medical UniversitySchool of Clinical Medicine, Ningxia Medical UniversitySchool of Clinical Medicine, Ningxia Medical UniversityDepartment of Pathology, Ningxia Medical UniversityDepartment of Hepatobiliary Surgery, General Hospital of Ningxia Medical UniversityDepartment of Hepatobiliary Surgery, General Hospital of Ningxia Medical UniversityAbstract Background Pancreatic cancer (PC) is a highly aggressive malignancy with a poor prognosis due to its late-stage diagnosis and limited treatment options. Objectives This study aimed to elucidate the molecular mechanisms underlying PC progression and identify potential molecular targets for its diagnosis and treatment. Methods DAZAP1 expression in PC tissues, normal tissues and cell lines was assessed using immunohistochemistry (IHC), reverse transcription–quantitative polymerase chain reaction (RT–qPCR) and western blotting. DAZAP1 knockdown was achieved through plasmid transfection, and its effects on ferroptosis and PC progression were evaluated using RT–qPCR, western blotting, CCK-8 assays, EdU staining, Fe2+ content measurement, reactive oxygen species (ROS) detection, wound healing and Transwell migration assays. Results DAZAP1 expression was significantly upregulated in PC tissues and cell lines compared to normal counterparts. DAZAP1 knockdown suppressed PC cell proliferation and induced ferroptosis, while ferroptosis inhibition reversed these effects, enhancing PC cell proliferation and metastasis. Conclusions DAZAP1 suppression promotes ferroptosis, thereby inhibiting PC cell proliferation and metastasis. These findings suggest that DAZAP1 is a potential therapeutic target for PC.https://doi.org/10.1186/s40001-024-02261-0DAZAP1FerroptosisProliferationMetastasisPancreatic cancer |
| spellingShingle | Xinqing Wang Hao Fan Xiaoping Ye Yu Hu Yan Xiao Ming Zhang Yonghui Xu Jianjun Song Yongyun Luo RNA-binding protein DAZAP1 accelerates the advancement of pancreatic cancer by inhibiting ferroptosis European Journal of Medical Research DAZAP1 Ferroptosis Proliferation Metastasis Pancreatic cancer |
| title | RNA-binding protein DAZAP1 accelerates the advancement of pancreatic cancer by inhibiting ferroptosis |
| title_full | RNA-binding protein DAZAP1 accelerates the advancement of pancreatic cancer by inhibiting ferroptosis |
| title_fullStr | RNA-binding protein DAZAP1 accelerates the advancement of pancreatic cancer by inhibiting ferroptosis |
| title_full_unstemmed | RNA-binding protein DAZAP1 accelerates the advancement of pancreatic cancer by inhibiting ferroptosis |
| title_short | RNA-binding protein DAZAP1 accelerates the advancement of pancreatic cancer by inhibiting ferroptosis |
| title_sort | rna binding protein dazap1 accelerates the advancement of pancreatic cancer by inhibiting ferroptosis |
| topic | DAZAP1 Ferroptosis Proliferation Metastasis Pancreatic cancer |
| url | https://doi.org/10.1186/s40001-024-02261-0 |
| work_keys_str_mv | AT xinqingwang rnabindingproteindazap1acceleratestheadvancementofpancreaticcancerbyinhibitingferroptosis AT haofan rnabindingproteindazap1acceleratestheadvancementofpancreaticcancerbyinhibitingferroptosis AT xiaopingye rnabindingproteindazap1acceleratestheadvancementofpancreaticcancerbyinhibitingferroptosis AT yuhu rnabindingproteindazap1acceleratestheadvancementofpancreaticcancerbyinhibitingferroptosis AT yanxiao rnabindingproteindazap1acceleratestheadvancementofpancreaticcancerbyinhibitingferroptosis AT mingzhang rnabindingproteindazap1acceleratestheadvancementofpancreaticcancerbyinhibitingferroptosis AT yonghuixu rnabindingproteindazap1acceleratestheadvancementofpancreaticcancerbyinhibitingferroptosis AT jianjunsong rnabindingproteindazap1acceleratestheadvancementofpancreaticcancerbyinhibitingferroptosis AT yongyunluo rnabindingproteindazap1acceleratestheadvancementofpancreaticcancerbyinhibitingferroptosis |