Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model

The aim of this study was to assess the osteoconductive and osteogenic properties of processed bovine dentin using a robust rabbit calvarial defect model. In total, 16 New Zealand White rabbits were operated to create three circular defects in the calvaria. One defect was left unfilled, one filled w...

Full description

Saved in:
Bibliographic Details
Main Authors: Ibrahim Hussain, Keyvan Moharamzadeh, Ian M. Brook, Patrício José de Oliveira Neto, Luiz A. Salata
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:International Journal of Dentistry
Online Access:http://dx.doi.org/10.1155/2012/396316
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850238118130614272
author Ibrahim Hussain
Keyvan Moharamzadeh
Ian M. Brook
Patrício José de Oliveira Neto
Luiz A. Salata
author_facet Ibrahim Hussain
Keyvan Moharamzadeh
Ian M. Brook
Patrício José de Oliveira Neto
Luiz A. Salata
author_sort Ibrahim Hussain
collection DOAJ
description The aim of this study was to assess the osteoconductive and osteogenic properties of processed bovine dentin using a robust rabbit calvarial defect model. In total, 16 New Zealand White rabbits were operated to create three circular defects in the calvaria. One defect was left unfilled, one filled with collected autogenous bone, and the third defect was filled with the dentin-based bone substitute. Following surgery and after a healing period of either 1 or 6 weeks, a CT scan was obtained. Following sacrificing, the tissues were processed for histological examination. The CT data showed the density in the area grafted with the dentin-based material was higher than the surrounding bone and the areas grafted with autologous bone after 1 week and 6 weeks of healing. The area left unfilled remained an empty defect after 1 week and 6 weeks. Histological examination of the defects filled with the dentin product after 6 weeks showed soft tissue encapsulation around the dentin particles. It can be concluded that the rabbit calvarial model used in this study is a robust model for the assessment of bone materials. Bovine dentin is a biostable material; however, it may not be suitable for repairing large 4-wall defects.
format Article
id doaj-art-07a83eaf9d694c2aab79a981c028b261
institution OA Journals
issn 1687-8728
1687-8736
language English
publishDate 2012-01-01
publisher Wiley
record_format Article
series International Journal of Dentistry
spelling doaj-art-07a83eaf9d694c2aab79a981c028b2612025-08-20T02:01:34ZengWileyInternational Journal of Dentistry1687-87281687-87362012-01-01201210.1155/2012/396316396316Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect ModelIbrahim Hussain0Keyvan Moharamzadeh1Ian M. Brook2Patrício José de Oliveira Neto3Luiz A. Salata4School of Clinical Dentistry, The University of Sheffield, Claremont Crescent, Sheffield S10 2TA, UKSchool of Clinical Dentistry, The University of Sheffield, Claremont Crescent, Sheffield S10 2TA, UKSchool of Clinical Dentistry, The University of Sheffield, Claremont Crescent, Sheffield S10 2TA, UKDepartment of Oral and Maxillofacial Surgery and Periodontics, Faculty of Dentistry of Ribeirao Preto, University of São Paulo, Avenida do Cafe s/n—Campus USP, 14040-904 Ribeirão Preto, SP, BrazilDepartment of Oral and Maxillofacial Surgery and Periodontics, Faculty of Dentistry of Ribeirao Preto, University of São Paulo, Avenida do Cafe s/n—Campus USP, 14040-904 Ribeirão Preto, SP, BrazilThe aim of this study was to assess the osteoconductive and osteogenic properties of processed bovine dentin using a robust rabbit calvarial defect model. In total, 16 New Zealand White rabbits were operated to create three circular defects in the calvaria. One defect was left unfilled, one filled with collected autogenous bone, and the third defect was filled with the dentin-based bone substitute. Following surgery and after a healing period of either 1 or 6 weeks, a CT scan was obtained. Following sacrificing, the tissues were processed for histological examination. The CT data showed the density in the area grafted with the dentin-based material was higher than the surrounding bone and the areas grafted with autologous bone after 1 week and 6 weeks of healing. The area left unfilled remained an empty defect after 1 week and 6 weeks. Histological examination of the defects filled with the dentin product after 6 weeks showed soft tissue encapsulation around the dentin particles. It can be concluded that the rabbit calvarial model used in this study is a robust model for the assessment of bone materials. Bovine dentin is a biostable material; however, it may not be suitable for repairing large 4-wall defects.http://dx.doi.org/10.1155/2012/396316
spellingShingle Ibrahim Hussain
Keyvan Moharamzadeh
Ian M. Brook
Patrício José de Oliveira Neto
Luiz A. Salata
Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model
International Journal of Dentistry
title Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model
title_full Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model
title_fullStr Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model
title_full_unstemmed Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model
title_short Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model
title_sort evaluation of osteoconductive and osteogenic potential of a dentin based bone substitute using a calvarial defect model
url http://dx.doi.org/10.1155/2012/396316
work_keys_str_mv AT ibrahimhussain evaluationofosteoconductiveandosteogenicpotentialofadentinbasedbonesubstituteusingacalvarialdefectmodel
AT keyvanmoharamzadeh evaluationofosteoconductiveandosteogenicpotentialofadentinbasedbonesubstituteusingacalvarialdefectmodel
AT ianmbrook evaluationofosteoconductiveandosteogenicpotentialofadentinbasedbonesubstituteusingacalvarialdefectmodel
AT patriciojosedeoliveiraneto evaluationofosteoconductiveandosteogenicpotentialofadentinbasedbonesubstituteusingacalvarialdefectmodel
AT luizasalata evaluationofosteoconductiveandosteogenicpotentialofadentinbasedbonesubstituteusingacalvarialdefectmodel