Mechanoregulation of Cell Fate by Low-intensity Pulsed Ultrasound: Mechanisms and Advances in Regenerative Medicine

As a mechanical wave capable of transmitting thermal and mechanical energy, ultrasound has emerged as a pivotal tool in regenerative medicine due to its non-invasive nature. Low-intensity pulsed ultrasound (LIPUS), a mechanoregulatory technique independent of thermal effects, delivers controlled mec...

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Main Authors: Lingling Lei, Qing Zhang, Meng Du, Li Li
Format: Article
Language:English
Published: Compuscript Ltd 2025-07-01
Series:BIO Integration
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Online Access:https://www.scienceopen.com/hosted-document?doi=10.15212/bioi-2025-0049
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author Lingling Lei
Qing Zhang
Meng Du
Li Li
author_facet Lingling Lei
Qing Zhang
Meng Du
Li Li
author_sort Lingling Lei
collection DOAJ
description As a mechanical wave capable of transmitting thermal and mechanical energy, ultrasound has emerged as a pivotal tool in regenerative medicine due to its non-invasive nature. Low-intensity pulsed ultrasound (LIPUS), a mechanoregulatory technique independent of thermal effects, delivers controlled mechanical stimuli to activate endogenous mechanotransduction pathways, such as ion channels, transmembrane proteins, and cytoskeleton-mediated signaling cascades. These pathways regulate critical cellular processes, such as proliferation, differentiation, and apoptosis, positioning LIPUS as a promising modality for targeted modulation of cell fate. Preclinical and clinical studies have demonstrated the therapeutic efficacy of LIPUS across diverse applications, including bone repair, neural regeneration, and soft tissue rehabilitation. However, optimizing stimulation parameters and advancing clinical translation remain key challenges. This review summarizes the central role of LIPUS in promoting tissue regeneration through non-thermal regulation of cellular homeostasis and explores strategies to accelerate clinical adoption of LIPUS. By integrating mechanistic insights with translational perspectives, this review provides a roadmap for advancing LIPUS-driven regenerative medicine in the era of precision bioengineering.
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spelling doaj-art-07a5695ebd954b01a09efdf4aa8c2f042025-08-20T03:15:35ZengCompuscript LtdBIO Integration2712-00822025-07-016110.15212/bioi-2025-0049e979Mechanoregulation of Cell Fate by Low-intensity Pulsed Ultrasound: Mechanisms and Advances in Regenerative MedicineLingling Lei0Qing Zhang1Meng Du2Li Li3Key Laboratory of Medical Imaging Precision Theranostics and Radiation Protection, College of Hunan Province, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, ChinaKey Laboratory of Medical Imaging Precision Theranostics and Radiation Protection, College of Hunan Province, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, ChinaKey Laboratory of Medical Imaging Precision Theranostics and Radiation Protection, College of Hunan Province, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, ChinaGulbali Institute, Charles Sturt University, Wagga Wagga, NSW 2678, AustraliaAs a mechanical wave capable of transmitting thermal and mechanical energy, ultrasound has emerged as a pivotal tool in regenerative medicine due to its non-invasive nature. Low-intensity pulsed ultrasound (LIPUS), a mechanoregulatory technique independent of thermal effects, delivers controlled mechanical stimuli to activate endogenous mechanotransduction pathways, such as ion channels, transmembrane proteins, and cytoskeleton-mediated signaling cascades. These pathways regulate critical cellular processes, such as proliferation, differentiation, and apoptosis, positioning LIPUS as a promising modality for targeted modulation of cell fate. Preclinical and clinical studies have demonstrated the therapeutic efficacy of LIPUS across diverse applications, including bone repair, neural regeneration, and soft tissue rehabilitation. However, optimizing stimulation parameters and advancing clinical translation remain key challenges. This review summarizes the central role of LIPUS in promoting tissue regeneration through non-thermal regulation of cellular homeostasis and explores strategies to accelerate clinical adoption of LIPUS. By integrating mechanistic insights with translational perspectives, this review provides a roadmap for advancing LIPUS-driven regenerative medicine in the era of precision bioengineering.https://www.scienceopen.com/hosted-document?doi=10.15212/bioi-2025-0049biological effectsdental treatmentfracture healinglow-intensity pulsed ultrasoundneuromodulationregenerative medicineurologic condition
spellingShingle Lingling Lei
Qing Zhang
Meng Du
Li Li
Mechanoregulation of Cell Fate by Low-intensity Pulsed Ultrasound: Mechanisms and Advances in Regenerative Medicine
BIO Integration
biological effects
dental treatment
fracture healing
low-intensity pulsed ultrasound
neuromodulation
regenerative medicine
urologic condition
title Mechanoregulation of Cell Fate by Low-intensity Pulsed Ultrasound: Mechanisms and Advances in Regenerative Medicine
title_full Mechanoregulation of Cell Fate by Low-intensity Pulsed Ultrasound: Mechanisms and Advances in Regenerative Medicine
title_fullStr Mechanoregulation of Cell Fate by Low-intensity Pulsed Ultrasound: Mechanisms and Advances in Regenerative Medicine
title_full_unstemmed Mechanoregulation of Cell Fate by Low-intensity Pulsed Ultrasound: Mechanisms and Advances in Regenerative Medicine
title_short Mechanoregulation of Cell Fate by Low-intensity Pulsed Ultrasound: Mechanisms and Advances in Regenerative Medicine
title_sort mechanoregulation of cell fate by low intensity pulsed ultrasound mechanisms and advances in regenerative medicine
topic biological effects
dental treatment
fracture healing
low-intensity pulsed ultrasound
neuromodulation
regenerative medicine
urologic condition
url https://www.scienceopen.com/hosted-document?doi=10.15212/bioi-2025-0049
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AT qingzhang mechanoregulationofcellfatebylowintensitypulsedultrasoundmechanismsandadvancesinregenerativemedicine
AT mengdu mechanoregulationofcellfatebylowintensitypulsedultrasoundmechanismsandadvancesinregenerativemedicine
AT lili mechanoregulationofcellfatebylowintensitypulsedultrasoundmechanismsandadvancesinregenerativemedicine