Gut Barrier Dysfunction and Microbiota Variations in Cryptosporidiosis: A Comprehensive Review

Cryptosporidiosis is a zoonotic protozoan parasite-born disease, equally significant in both animals and humans, especially affecting immunocompromised individuals (e.g., AIDS patients) and neonates. The prime concerns of this review article are to demonstrate the disruption of the intestinal barrie...

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Main Authors: Munwar Ali, Chang Xu, Mingyue Wang, Qazal Hina, Yaru Ji, Subiha Anwar, Sijia Lu, Qing He, Yawei Qiu, Kun Li
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Veterinary Sciences
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Online Access:https://www.mdpi.com/2306-7381/12/2/85
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author Munwar Ali
Chang Xu
Mingyue Wang
Qazal Hina
Yaru Ji
Subiha Anwar
Sijia Lu
Qing He
Yawei Qiu
Kun Li
author_facet Munwar Ali
Chang Xu
Mingyue Wang
Qazal Hina
Yaru Ji
Subiha Anwar
Sijia Lu
Qing He
Yawei Qiu
Kun Li
author_sort Munwar Ali
collection DOAJ
description Cryptosporidiosis is a zoonotic protozoan parasite-born disease, equally significant in both animals and humans, especially affecting immunocompromised individuals (e.g., AIDS patients) and neonates. The prime concerns of this review article are to demonstrate the disruption of the intestinal barrier and variations in the gut microbiome during cryptosporidiosis, and to explore host gut–parasite interactions that can lead to the development of novel therapeutics. The review concluded that the enteric barrier is particularly maintained by tight junction proteins (e.g., occludin, claudin, and ZO-1, etc.) and mucosal immunity, both of which are severely compromised during <i>Cryptosporidium</i> spp. infections, resulting in increased intestinal barrier permeability, inflammatory responses, diarrhea, and ultimately death in severe cases. <i>Cryptosporidium</i>-induced dysbiosis is characterized by reduced microbial diversity and richness, a shift from commensal to pathogenic bacteria, as evidenced by increased pro-inflammatory taxa like Proteobacteria, and reduced proportions of beneficial SCFAs producing bacteria, e.g., Firmicutes. Recent investigations have highlighted the interrelations between gut microbiota and epithelial barrier integrity, especially during cryptosporidiosis, demonstrating the modulations regarding tight junctions (TJs), immune reactions, and SCFA production, all of which are main players in alleviating this protozoal parasitic infection. This review comprehensively describes the fine details underlying these impairments, including autophagy-mediated TJs’ degradation, inflammasome activation, and gut microbiome-driven alterations in metabolic pathways, providing the latest relevant, and well-organized piece of knowledge regarding intestinal barrier alterations and microbial shifts during cryptosporidiosis. This work emphasizes the future need for longitudinal studies and advanced sequencing techniques to understand host gut microbiota–parasite interactions, aiming to formulate innovative strategies to mitigate cryptosporidiosis.
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issn 2306-7381
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series Veterinary Sciences
spelling doaj-art-079eb8ec34b249c08455d1f07fc162182025-08-20T02:03:31ZengMDPI AGVeterinary Sciences2306-73812025-01-011228510.3390/vetsci12020085Gut Barrier Dysfunction and Microbiota Variations in Cryptosporidiosis: A Comprehensive ReviewMunwar Ali0Chang Xu1Mingyue Wang2Qazal Hina3Yaru Ji4Subiha Anwar5Sijia Lu6Qing He7Yawei Qiu8Kun Li9College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Animal Nutrition, University of Veterinary and Animal Sciences, Lahore 54000, PakistanCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Animal Husbandry, University of Agriculture, Faisalabad 38000, PakistanCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaCryptosporidiosis is a zoonotic protozoan parasite-born disease, equally significant in both animals and humans, especially affecting immunocompromised individuals (e.g., AIDS patients) and neonates. The prime concerns of this review article are to demonstrate the disruption of the intestinal barrier and variations in the gut microbiome during cryptosporidiosis, and to explore host gut–parasite interactions that can lead to the development of novel therapeutics. The review concluded that the enteric barrier is particularly maintained by tight junction proteins (e.g., occludin, claudin, and ZO-1, etc.) and mucosal immunity, both of which are severely compromised during <i>Cryptosporidium</i> spp. infections, resulting in increased intestinal barrier permeability, inflammatory responses, diarrhea, and ultimately death in severe cases. <i>Cryptosporidium</i>-induced dysbiosis is characterized by reduced microbial diversity and richness, a shift from commensal to pathogenic bacteria, as evidenced by increased pro-inflammatory taxa like Proteobacteria, and reduced proportions of beneficial SCFAs producing bacteria, e.g., Firmicutes. Recent investigations have highlighted the interrelations between gut microbiota and epithelial barrier integrity, especially during cryptosporidiosis, demonstrating the modulations regarding tight junctions (TJs), immune reactions, and SCFA production, all of which are main players in alleviating this protozoal parasitic infection. This review comprehensively describes the fine details underlying these impairments, including autophagy-mediated TJs’ degradation, inflammasome activation, and gut microbiome-driven alterations in metabolic pathways, providing the latest relevant, and well-organized piece of knowledge regarding intestinal barrier alterations and microbial shifts during cryptosporidiosis. This work emphasizes the future need for longitudinal studies and advanced sequencing techniques to understand host gut microbiota–parasite interactions, aiming to formulate innovative strategies to mitigate cryptosporidiosis.https://www.mdpi.com/2306-7381/12/2/85<i>Cryptosporidium</i>protozoanzoonoticgutintestinal barriermicrobiota
spellingShingle Munwar Ali
Chang Xu
Mingyue Wang
Qazal Hina
Yaru Ji
Subiha Anwar
Sijia Lu
Qing He
Yawei Qiu
Kun Li
Gut Barrier Dysfunction and Microbiota Variations in Cryptosporidiosis: A Comprehensive Review
Veterinary Sciences
<i>Cryptosporidium</i>
protozoan
zoonotic
gut
intestinal barrier
microbiota
title Gut Barrier Dysfunction and Microbiota Variations in Cryptosporidiosis: A Comprehensive Review
title_full Gut Barrier Dysfunction and Microbiota Variations in Cryptosporidiosis: A Comprehensive Review
title_fullStr Gut Barrier Dysfunction and Microbiota Variations in Cryptosporidiosis: A Comprehensive Review
title_full_unstemmed Gut Barrier Dysfunction and Microbiota Variations in Cryptosporidiosis: A Comprehensive Review
title_short Gut Barrier Dysfunction and Microbiota Variations in Cryptosporidiosis: A Comprehensive Review
title_sort gut barrier dysfunction and microbiota variations in cryptosporidiosis a comprehensive review
topic <i>Cryptosporidium</i>
protozoan
zoonotic
gut
intestinal barrier
microbiota
url https://www.mdpi.com/2306-7381/12/2/85
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