Gram-negative bacilli causing infections in an intensive care unit of a tertiary care hospital in Istanbul, Turkey

Introduction: This study aimed to demonstrate the changing epidemiology of infecting microorganisms and their long-term resistance profiles and to describe the microbiological point of view in anti-infective management of intensive care unit (ICU) patients. Methodology: A total of 5,690 isolates of...

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Main Authors: Seniha Senbayrak Akcay, Asuman Inan, Simin Cevan, Ayse Nilufer Ozaydın, Naz Cobanoglu, Seyfi Celik Ozyurek, Sebahat Aksaray
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2014-05-01
Series:Journal of Infection in Developing Countries
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Online Access:https://jidc.org/index.php/journal/article/view/4277
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author Seniha Senbayrak Akcay
Asuman Inan
Simin Cevan
Ayse Nilufer Ozaydın
Naz Cobanoglu
Seyfi Celik Ozyurek
Sebahat Aksaray
author_facet Seniha Senbayrak Akcay
Asuman Inan
Simin Cevan
Ayse Nilufer Ozaydın
Naz Cobanoglu
Seyfi Celik Ozyurek
Sebahat Aksaray
author_sort Seniha Senbayrak Akcay
collection DOAJ
description Introduction: This study aimed to demonstrate the changing epidemiology of infecting microorganisms and their long-term resistance profiles and to describe the microbiological point of view in anti-infective management of intensive care unit (ICU) patients. Methodology: A total of 5,690 isolates of Gram-negative bacilli were included in this study. Antibiotic susceptibility was tested using the disk diffusion method and Vitek 2 system. Chi-square tests were used for hypothesis testing. Results: The most frequently isolated organisms were A. baumannii (37.3%), P. aeruginosa (30.3%), Enterobacter spp. (10.4%), E. coli (10.4%), and Klebsiella spp. (8.9%). A. baumannii was the most frequently isolated organism from the respiratory tract (43.4%); the susceptibility rates for imipenem and meropenem decreased to 7% and 6% (p < 0.0001), respectively. The percentage of multidrug-resistant (MDR) A. baumannii isolates continuously increased from 18.7% in 2004 to 69% in 2011 (p < 0.0001), whereas MDR P. aeruginosa isolates increased from 1.5% to 22% (p < 0.0001). Carbapenem-resistant Klebsiella isolates emerged in 2010 and increased to 20% in the next year. The rates of ESBL-producing Enterobacteriaceae in the ICU was very high in 2011 – 50% for E. coli and 80% for Klebsiella strains. Conclusion: The most common isolated Gram-negative bacillus in our study was A. baumannii and that the prevalence of MDR isolates has increased markedly over. Accordingly, the comparison of antibiotic resistance of other pathogens in 2004 and 2011 displayed an increasing trend. These data imply the urgent need for new and effective strategies in our hospital and in the region.
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spelling doaj-art-079de2c9dacb424fbc54b8209bcdc32e2025-08-20T02:14:19ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802014-05-0180510.3855/jidc.4277Gram-negative bacilli causing infections in an intensive care unit of a tertiary care hospital in Istanbul, TurkeySeniha Senbayrak Akcay0Asuman Inan1Simin Cevan2Ayse Nilufer Ozaydın3Naz Cobanoglu4Seyfi Celik Ozyurek5Sebahat Aksaray6Haydarpasa Numune Education and Research Hospital, Uskudar, Istanbul, TurkeyHaydarpasa Numune Education and Research Hospital, Uskudar, Istanbul, TurkeyHaydarpasa Numune Education and Research Hospital, Uskudar, Istanbul, TurkeyMarmara University, Faculty of Medicine, Uskudar, Istanbul, TurkeyHaydarpasa Numune Education and Research Hospital, Uskudar, Istanbul, TurkeyHaydarpasa Numune Education and Research Hospital, Uskudar, Istanbul, TurkeyHaydarpasa Numune Education and Research Hospital, Uskudar, Istanbul, TurkeyIntroduction: This study aimed to demonstrate the changing epidemiology of infecting microorganisms and their long-term resistance profiles and to describe the microbiological point of view in anti-infective management of intensive care unit (ICU) patients. Methodology: A total of 5,690 isolates of Gram-negative bacilli were included in this study. Antibiotic susceptibility was tested using the disk diffusion method and Vitek 2 system. Chi-square tests were used for hypothesis testing. Results: The most frequently isolated organisms were A. baumannii (37.3%), P. aeruginosa (30.3%), Enterobacter spp. (10.4%), E. coli (10.4%), and Klebsiella spp. (8.9%). A. baumannii was the most frequently isolated organism from the respiratory tract (43.4%); the susceptibility rates for imipenem and meropenem decreased to 7% and 6% (p < 0.0001), respectively. The percentage of multidrug-resistant (MDR) A. baumannii isolates continuously increased from 18.7% in 2004 to 69% in 2011 (p < 0.0001), whereas MDR P. aeruginosa isolates increased from 1.5% to 22% (p < 0.0001). Carbapenem-resistant Klebsiella isolates emerged in 2010 and increased to 20% in the next year. The rates of ESBL-producing Enterobacteriaceae in the ICU was very high in 2011 – 50% for E. coli and 80% for Klebsiella strains. Conclusion: The most common isolated Gram-negative bacillus in our study was A. baumannii and that the prevalence of MDR isolates has increased markedly over. Accordingly, the comparison of antibiotic resistance of other pathogens in 2004 and 2011 displayed an increasing trend. These data imply the urgent need for new and effective strategies in our hospital and in the region. https://jidc.org/index.php/journal/article/view/4277Gram-negative bacilliAntibiotic resistanceICUMDR
spellingShingle Seniha Senbayrak Akcay
Asuman Inan
Simin Cevan
Ayse Nilufer Ozaydın
Naz Cobanoglu
Seyfi Celik Ozyurek
Sebahat Aksaray
Gram-negative bacilli causing infections in an intensive care unit of a tertiary care hospital in Istanbul, Turkey
Journal of Infection in Developing Countries
Gram-negative bacilli
Antibiotic resistance
ICU
MDR
title Gram-negative bacilli causing infections in an intensive care unit of a tertiary care hospital in Istanbul, Turkey
title_full Gram-negative bacilli causing infections in an intensive care unit of a tertiary care hospital in Istanbul, Turkey
title_fullStr Gram-negative bacilli causing infections in an intensive care unit of a tertiary care hospital in Istanbul, Turkey
title_full_unstemmed Gram-negative bacilli causing infections in an intensive care unit of a tertiary care hospital in Istanbul, Turkey
title_short Gram-negative bacilli causing infections in an intensive care unit of a tertiary care hospital in Istanbul, Turkey
title_sort gram negative bacilli causing infections in an intensive care unit of a tertiary care hospital in istanbul turkey
topic Gram-negative bacilli
Antibiotic resistance
ICU
MDR
url https://jidc.org/index.php/journal/article/view/4277
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