Anti-DENV-ED3 antibody cross-talks generate immune interference among the four DENVs
Objective: To evaluate the effects of primary anti-dengue virus envelop protein domain 3 (DENV-ED3) antibodies on secondary heterotypic anti-DENV ED3 antibody responses and the status of anti-DENV antibody responses against multivalent DENV ED3s in mice. Methods: Four different DENV-ED3s were purifi...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Asian Pacific Journal of Tropical Medicine |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/apjtm.apjtm_257_24 |
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| author | Md. Din Islam Sanjida Yesmin Tahmina Sharmin Md. Ayoub Khan Yutaka Kuroda M. Monirul Islam |
| author_facet | Md. Din Islam Sanjida Yesmin Tahmina Sharmin Md. Ayoub Khan Yutaka Kuroda M. Monirul Islam |
| author_sort | Md. Din Islam |
| collection | DOAJ |
| description | Objective:
To evaluate the effects of primary anti-dengue virus envelop protein domain 3 (DENV-ED3) antibodies on secondary heterotypic anti-DENV ED3 antibody responses and the status of anti-DENV antibody responses against multivalent DENV ED3s in mice.
Methods:
Four different DENV-ED3s were purified and their biophysical characteristics were confirmed. Swiss albino mice aged 3-4 weeks were immunized with four different DENV-ED3s and the anti-ED3 IgG responses were determined by ELISA.
Results:
Firstly, the primary 1ED3-2ED3-3ED3 cross-reactive anti-DENV1 ED3 response boosted the secondary anti-2ED3 and anti-3ED3 antibody responses. In contrast, primary anti-2ED3 and anti-3ED3 antibodies neither had cross-recognition of 1ED3, nor had any effect on secondary anti-1ED3 response. Besides, the strict serospecificity of the anti-4ED3 sera did not affect other secondary anti-DENV ED3 responses. Secondly, 1ED3, 2ED3, and 3ED3 were co-dominantly immunogenic in trivalent ED3 formulations. However, the poorly immunogenic 4ED3 became almost non-immunogenic when injected after or together with 2ED3 and 3ED3, but showed slightly increased immunogenicity when injected with 1ED3, suggesting an adjuvanticity of 1ED3 on 4ED3’s immunogenicity.
Conclusions:
Although DENV1~4 ED3s share similar sequence homologies and structures, their immune induction potentials differ significantly in terms of immune magnitude, sero-specificity, and sero-cross-reactivity. Such intrinsic features of DENV1~4 ED3s may lead to ‘antigen interference’, limiting both the understanding of dengue etiology and the success of dengue vaccine development, which needs to neutralize all four DENV serotypes equivalently. |
| format | Article |
| id | doaj-art-078691a5978e464b86cd763115cdc388 |
| institution | Kabale University |
| issn | 2352-4146 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Medicine |
| spelling | doaj-art-078691a5978e464b86cd763115cdc3882025-01-07T04:55:34ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Medicine2352-41462024-12-01171255356210.4103/apjtm.apjtm_257_24Anti-DENV-ED3 antibody cross-talks generate immune interference among the four DENVsMd. Din IslamSanjida YesminTahmina SharminMd. Ayoub KhanYutaka KurodaM. Monirul IslamObjective: To evaluate the effects of primary anti-dengue virus envelop protein domain 3 (DENV-ED3) antibodies on secondary heterotypic anti-DENV ED3 antibody responses and the status of anti-DENV antibody responses against multivalent DENV ED3s in mice. Methods: Four different DENV-ED3s were purified and their biophysical characteristics were confirmed. Swiss albino mice aged 3-4 weeks were immunized with four different DENV-ED3s and the anti-ED3 IgG responses were determined by ELISA. Results: Firstly, the primary 1ED3-2ED3-3ED3 cross-reactive anti-DENV1 ED3 response boosted the secondary anti-2ED3 and anti-3ED3 antibody responses. In contrast, primary anti-2ED3 and anti-3ED3 antibodies neither had cross-recognition of 1ED3, nor had any effect on secondary anti-1ED3 response. Besides, the strict serospecificity of the anti-4ED3 sera did not affect other secondary anti-DENV ED3 responses. Secondly, 1ED3, 2ED3, and 3ED3 were co-dominantly immunogenic in trivalent ED3 formulations. However, the poorly immunogenic 4ED3 became almost non-immunogenic when injected after or together with 2ED3 and 3ED3, but showed slightly increased immunogenicity when injected with 1ED3, suggesting an adjuvanticity of 1ED3 on 4ED3’s immunogenicity. Conclusions: Although DENV1~4 ED3s share similar sequence homologies and structures, their immune induction potentials differ significantly in terms of immune magnitude, sero-specificity, and sero-cross-reactivity. Such intrinsic features of DENV1~4 ED3s may lead to ‘antigen interference’, limiting both the understanding of dengue etiology and the success of dengue vaccine development, which needs to neutralize all four DENV serotypes equivalently.https://journals.lww.com/10.4103/apjtm.apjtm_257_24dengue viruses (denvs)denv sero-specificitydenv serotype-cross-talksprimary denv infectionssecondary denv infectionsmultivalent denv-ed3s |
| spellingShingle | Md. Din Islam Sanjida Yesmin Tahmina Sharmin Md. Ayoub Khan Yutaka Kuroda M. Monirul Islam Anti-DENV-ED3 antibody cross-talks generate immune interference among the four DENVs Asian Pacific Journal of Tropical Medicine dengue viruses (denvs) denv sero-specificity denv serotype-cross-talks primary denv infections secondary denv infections multivalent denv-ed3s |
| title | Anti-DENV-ED3 antibody cross-talks generate immune interference among the four DENVs |
| title_full | Anti-DENV-ED3 antibody cross-talks generate immune interference among the four DENVs |
| title_fullStr | Anti-DENV-ED3 antibody cross-talks generate immune interference among the four DENVs |
| title_full_unstemmed | Anti-DENV-ED3 antibody cross-talks generate immune interference among the four DENVs |
| title_short | Anti-DENV-ED3 antibody cross-talks generate immune interference among the four DENVs |
| title_sort | anti denv ed3 antibody cross talks generate immune interference among the four denvs |
| topic | dengue viruses (denvs) denv sero-specificity denv serotype-cross-talks primary denv infections secondary denv infections multivalent denv-ed3s |
| url | https://journals.lww.com/10.4103/apjtm.apjtm_257_24 |
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