Genome-wide identification and expression analysis of the AS2/LOB gene family in physic nut

IntroductionAS2/LOB genes, a class of transcription factors ubiquitously existing in plants, are vital for plant growth, development, and stress tolerance. Despite the availability of the physic nut genome, information regarding the expression profiles and evolutionary histories of its AS2/LOB genes...

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Main Authors: Yuehui Tang, Xiaohui Wang, Jiayu Feng, Yaoyao Wang, Tengfei Liu, Xinxin Bao
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-12-01
Series:Frontiers in Plant Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2024.1504093/full
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author Yuehui Tang
Xiaohui Wang
Jiayu Feng
Yaoyao Wang
Tengfei Liu
Xinxin Bao
author_facet Yuehui Tang
Xiaohui Wang
Jiayu Feng
Yaoyao Wang
Tengfei Liu
Xinxin Bao
author_sort Yuehui Tang
collection DOAJ
description IntroductionAS2/LOB genes, a class of transcription factors ubiquitously existing in plants, are vital for plant growth, development, and stress tolerance. Despite the availability of the physic nut genome, information regarding the expression profiles and evolutionary histories of its AS2/LOB genes remains scarce.MethodsAn elaborate exploration of the AS2/LOB gene family was conducted, including phylogeny, exon-intron structure, chromosomal location, conserved domain characteristics, conserved motifs, promoter cis-acting elements, protein interaction, and expression profiles under normal growth and abiotic stress conditions.ResultsIn this study, 28 AS2/LOB genes (JcASLs) were identified in the physic nut genome. Phylogenetic analysis, based on homologs from Arabidopsis, classified the 28 JcASLs genes into two groups (calss I and II). Chromosome localization indicated that the 28 JcASLs genes were unevenly distributed across nine chromosomes. RNA-seq and qRT-PCR results revealed that the majority of the 28 JcASLs genes exhibited differential expression in tissues such as roots, cortex stems, leaves, and seeds. Notably, JcASL8 and JcASL13 were exclusively expressed in seeds, and 16 JcASLs genes responded to drought and salt stress at least at one time point under at least one treatment condition.DiscussionThese results establish a basis for future investigations into the molecular mechanism by which the JcASLs genes regulate physic nut's response to drought and salt stress and their role in modulating the growth and development of physic nut.
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spelling doaj-art-0783baee13cb4e90952f83e95f9a937b2025-08-20T02:38:29ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-12-011510.3389/fpls.2024.15040931504093Genome-wide identification and expression analysis of the AS2/LOB gene family in physic nutYuehui TangXiaohui WangJiayu FengYaoyao WangTengfei LiuXinxin BaoIntroductionAS2/LOB genes, a class of transcription factors ubiquitously existing in plants, are vital for plant growth, development, and stress tolerance. Despite the availability of the physic nut genome, information regarding the expression profiles and evolutionary histories of its AS2/LOB genes remains scarce.MethodsAn elaborate exploration of the AS2/LOB gene family was conducted, including phylogeny, exon-intron structure, chromosomal location, conserved domain characteristics, conserved motifs, promoter cis-acting elements, protein interaction, and expression profiles under normal growth and abiotic stress conditions.ResultsIn this study, 28 AS2/LOB genes (JcASLs) were identified in the physic nut genome. Phylogenetic analysis, based on homologs from Arabidopsis, classified the 28 JcASLs genes into two groups (calss I and II). Chromosome localization indicated that the 28 JcASLs genes were unevenly distributed across nine chromosomes. RNA-seq and qRT-PCR results revealed that the majority of the 28 JcASLs genes exhibited differential expression in tissues such as roots, cortex stems, leaves, and seeds. Notably, JcASL8 and JcASL13 were exclusively expressed in seeds, and 16 JcASLs genes responded to drought and salt stress at least at one time point under at least one treatment condition.DiscussionThese results establish a basis for future investigations into the molecular mechanism by which the JcASLs genes regulate physic nut's response to drought and salt stress and their role in modulating the growth and development of physic nut.https://www.frontiersin.org/articles/10.3389/fpls.2024.1504093/fullAS2/LOB gene familyabiotic stressphysic nutexpression patternphylogenetic tree
spellingShingle Yuehui Tang
Xiaohui Wang
Jiayu Feng
Yaoyao Wang
Tengfei Liu
Xinxin Bao
Genome-wide identification and expression analysis of the AS2/LOB gene family in physic nut
Frontiers in Plant Science
AS2/LOB gene family
abiotic stress
physic nut
expression pattern
phylogenetic tree
title Genome-wide identification and expression analysis of the AS2/LOB gene family in physic nut
title_full Genome-wide identification and expression analysis of the AS2/LOB gene family in physic nut
title_fullStr Genome-wide identification and expression analysis of the AS2/LOB gene family in physic nut
title_full_unstemmed Genome-wide identification and expression analysis of the AS2/LOB gene family in physic nut
title_short Genome-wide identification and expression analysis of the AS2/LOB gene family in physic nut
title_sort genome wide identification and expression analysis of the as2 lob gene family in physic nut
topic AS2/LOB gene family
abiotic stress
physic nut
expression pattern
phylogenetic tree
url https://www.frontiersin.org/articles/10.3389/fpls.2024.1504093/full
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AT yaoyaowang genomewideidentificationandexpressionanalysisoftheas2lobgenefamilyinphysicnut
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