Bordetella hinzii Genomic Characteristics and Vaccine Target Discovery
This study was aimed at analyzing the pathogenic characteristics of Bordetella hinzii ( B. hinzii ), and elucidating its antibiotic resistance mechanisms, virulence gene distribution, and vaccine development potential. Genomes of 38 global strains were downloaded from the NCBI database. After qual...
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| Format: | Article |
| Language: | English |
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Compuscript Ltd
2025-06-01
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| Series: | Zoonoses |
| Online Access: | https://www.scienceopen.com/hosted-document?doi=10.15212/ZOONOSES-2025-0027 |
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| author | Jin Rui Mao Junjie Deng Yuhang Wu Wenwei Liang Xuechen Zheng Enhui Li Quwen |
| author_facet | Jin Rui Mao Junjie Deng Yuhang Wu Wenwei Liang Xuechen Zheng Enhui Li Quwen |
| author_sort | Jin Rui |
| collection | DOAJ |
| description | This study was aimed at analyzing the pathogenic characteristics of Bordetella hinzii ( B. hinzii ), and elucidating its antibiotic resistance mechanisms, virulence gene distribution, and vaccine development potential. Genomes of 38 global strains were downloaded from the NCBI database. After quality control with CheckM, pan-genome analysis was performed with Roary, SNP-based phylogenetic trees were constructed with snippy, and antibiotic resistance genes (CARD database) and virulence genes (VFDB database) were predicted with abricate. Bioinformatics tools were integrated to screen candidate vaccine proteins. All strains carried intrinsic resistance genes, including efflux pumps ( MexAB-OprM ) and the β-lactamase HBL-1 , which conferred resistance to ten antibiotic classes. Core virulence genes ( bplA-L , bvgAS , and flagella-associated genes) were highly conserved (detection rate >92.1%). SNP analysis classified the strains into six clades, and human-derived isolates showed host-specific clustering. The pan-genome was open (7,530 genes; Heaps’ law parameter B = 0.35). Two outer membrane proteins, LptD and BamD, were identified as vaccine candidates (antigenicity scores >0.4; no host homology). B. hinzii exhibits cross-host transmission risks, driven by conserved virulence genes and an open genome enabling adaptive evolution. LptD and BamD represent potential vaccine targets requiring further validation. This study provides a theoretical basis for clinical management and prevention strategies. |
| format | Article |
| id | doaj-art-077ae41e4a624ea892b3c3406cba1d3a |
| institution | Kabale University |
| issn | 2737-7466 2737-7474 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Compuscript Ltd |
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| series | Zoonoses |
| spelling | doaj-art-077ae41e4a624ea892b3c3406cba1d3a2025-08-20T03:51:48ZengCompuscript LtdZoonoses2737-74662737-74742025-06-015197310.15212/ZOONOSES-2025-0027Bordetella hinzii Genomic Characteristics and Vaccine Target DiscoveryJin RuiMao JunjieDeng YuhangWu WenweiLiang XuechenZheng EnhuiLi QuwenThis study was aimed at analyzing the pathogenic characteristics of Bordetella hinzii ( B. hinzii ), and elucidating its antibiotic resistance mechanisms, virulence gene distribution, and vaccine development potential. Genomes of 38 global strains were downloaded from the NCBI database. After quality control with CheckM, pan-genome analysis was performed with Roary, SNP-based phylogenetic trees were constructed with snippy, and antibiotic resistance genes (CARD database) and virulence genes (VFDB database) were predicted with abricate. Bioinformatics tools were integrated to screen candidate vaccine proteins. All strains carried intrinsic resistance genes, including efflux pumps ( MexAB-OprM ) and the β-lactamase HBL-1 , which conferred resistance to ten antibiotic classes. Core virulence genes ( bplA-L , bvgAS , and flagella-associated genes) were highly conserved (detection rate >92.1%). SNP analysis classified the strains into six clades, and human-derived isolates showed host-specific clustering. The pan-genome was open (7,530 genes; Heaps’ law parameter B = 0.35). Two outer membrane proteins, LptD and BamD, were identified as vaccine candidates (antigenicity scores >0.4; no host homology). B. hinzii exhibits cross-host transmission risks, driven by conserved virulence genes and an open genome enabling adaptive evolution. LptD and BamD represent potential vaccine targets requiring further validation. This study provides a theoretical basis for clinical management and prevention strategies.https://www.scienceopen.com/hosted-document?doi=10.15212/ZOONOSES-2025-0027 |
| spellingShingle | Jin Rui Mao Junjie Deng Yuhang Wu Wenwei Liang Xuechen Zheng Enhui Li Quwen Bordetella hinzii Genomic Characteristics and Vaccine Target Discovery Zoonoses |
| title | Bordetella hinzii Genomic Characteristics and Vaccine Target Discovery |
| title_full | Bordetella hinzii Genomic Characteristics and Vaccine Target Discovery |
| title_fullStr | Bordetella hinzii Genomic Characteristics and Vaccine Target Discovery |
| title_full_unstemmed | Bordetella hinzii Genomic Characteristics and Vaccine Target Discovery |
| title_short | Bordetella hinzii Genomic Characteristics and Vaccine Target Discovery |
| title_sort | bordetella hinzii genomic characteristics and vaccine target discovery |
| url | https://www.scienceopen.com/hosted-document?doi=10.15212/ZOONOSES-2025-0027 |
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