The Screening of Aptamers and the Development of a Colorimetric Detection Method for the Pesticide Deltamethrin
Deltamethrin (Del), a widely utilized pyrethroid pesticide, exhibits significant risks to human health due to its persistent environmental residues. This study aims to develop an efficient sensing detector for rapid Del detection through aptamer-based recognition. A modified Capture-SELEX strategy s...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/25/7/2060 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849730157660602368 |
|---|---|
| author | Caixia Wu Wenwei Li Jiafu Wang Sheng Li |
| author_facet | Caixia Wu Wenwei Li Jiafu Wang Sheng Li |
| author_sort | Caixia Wu |
| collection | DOAJ |
| description | Deltamethrin (Del), a widely utilized pyrethroid pesticide, exhibits significant risks to human health due to its persistent environmental residues. This study aims to develop an efficient sensing detector for rapid Del detection through aptamer-based recognition. A modified Capture-SELEX strategy successfully identified Del-1, a high-affinity DNA aptamer demonstrating specific binding to Del with a dissociation constant (Kd) of 82.90 ± 6.272 nM. Molecular docking analysis revealed strong intermolecular interactions between Del-1 and Del, exhibiting a favorable binding energy of −7.35 kcal·mol<sup>−1</sup>. Leveraging these findings, we constructed a colorimetric detector using gold nanoparticles (AuNPs) and poly dimethyl diallyl ammonium chloride (PDDA)-mediated aggregation modulation. The sensing detector employed dual detection parameters: (1) a characteristic color transition from red to blue and (2) a quantitative ∆A650/A520 ratio measurement. This optimized system achieved a detection limit of 54.57 ng·mL<sup>−1</sup> with exceptional specificity against other competitive pesticides. Practical validation using spiked fruit samples (apples and pears) yielded satisfactory recoveries of 74–118%, demonstrating the sensor’s reliability in real-sample analysis. The developed methodology presents a promising approach for the on-site monitoring of pyrethroid contaminants in agricultural products. |
| format | Article |
| id | doaj-art-076e40a319654d8ea3c7e8b9be50d418 |
| institution | DOAJ |
| issn | 1424-8220 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj-art-076e40a319654d8ea3c7e8b9be50d4182025-08-20T03:08:59ZengMDPI AGSensors1424-82202025-03-01257206010.3390/s25072060The Screening of Aptamers and the Development of a Colorimetric Detection Method for the Pesticide DeltamethrinCaixia Wu0Wenwei Li1Jiafu Wang2Sheng Li3Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Minister of Education), Department of Life Science/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, ChinaKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Minister of Education), Department of Life Science/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, ChinaKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Minister of Education), Department of Life Science/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, ChinaDepartment of Medical Technology, Chongqing Medical and Pharmaceutical College, Chongqing 400000, ChinaDeltamethrin (Del), a widely utilized pyrethroid pesticide, exhibits significant risks to human health due to its persistent environmental residues. This study aims to develop an efficient sensing detector for rapid Del detection through aptamer-based recognition. A modified Capture-SELEX strategy successfully identified Del-1, a high-affinity DNA aptamer demonstrating specific binding to Del with a dissociation constant (Kd) of 82.90 ± 6.272 nM. Molecular docking analysis revealed strong intermolecular interactions between Del-1 and Del, exhibiting a favorable binding energy of −7.35 kcal·mol<sup>−1</sup>. Leveraging these findings, we constructed a colorimetric detector using gold nanoparticles (AuNPs) and poly dimethyl diallyl ammonium chloride (PDDA)-mediated aggregation modulation. The sensing detector employed dual detection parameters: (1) a characteristic color transition from red to blue and (2) a quantitative ∆A650/A520 ratio measurement. This optimized system achieved a detection limit of 54.57 ng·mL<sup>−1</sup> with exceptional specificity against other competitive pesticides. Practical validation using spiked fruit samples (apples and pears) yielded satisfactory recoveries of 74–118%, demonstrating the sensor’s reliability in real-sample analysis. The developed methodology presents a promising approach for the on-site monitoring of pyrethroid contaminants in agricultural products.https://www.mdpi.com/1424-8220/25/7/2060deltamethrinaptamerCapture-SELEXcolorimetric sensorAuNPs |
| spellingShingle | Caixia Wu Wenwei Li Jiafu Wang Sheng Li The Screening of Aptamers and the Development of a Colorimetric Detection Method for the Pesticide Deltamethrin Sensors deltamethrin aptamer Capture-SELEX colorimetric sensor AuNPs |
| title | The Screening of Aptamers and the Development of a Colorimetric Detection Method for the Pesticide Deltamethrin |
| title_full | The Screening of Aptamers and the Development of a Colorimetric Detection Method for the Pesticide Deltamethrin |
| title_fullStr | The Screening of Aptamers and the Development of a Colorimetric Detection Method for the Pesticide Deltamethrin |
| title_full_unstemmed | The Screening of Aptamers and the Development of a Colorimetric Detection Method for the Pesticide Deltamethrin |
| title_short | The Screening of Aptamers and the Development of a Colorimetric Detection Method for the Pesticide Deltamethrin |
| title_sort | screening of aptamers and the development of a colorimetric detection method for the pesticide deltamethrin |
| topic | deltamethrin aptamer Capture-SELEX colorimetric sensor AuNPs |
| url | https://www.mdpi.com/1424-8220/25/7/2060 |
| work_keys_str_mv | AT caixiawu thescreeningofaptamersandthedevelopmentofacolorimetricdetectionmethodforthepesticidedeltamethrin AT wenweili thescreeningofaptamersandthedevelopmentofacolorimetricdetectionmethodforthepesticidedeltamethrin AT jiafuwang thescreeningofaptamersandthedevelopmentofacolorimetricdetectionmethodforthepesticidedeltamethrin AT shengli thescreeningofaptamersandthedevelopmentofacolorimetricdetectionmethodforthepesticidedeltamethrin AT caixiawu screeningofaptamersandthedevelopmentofacolorimetricdetectionmethodforthepesticidedeltamethrin AT wenweili screeningofaptamersandthedevelopmentofacolorimetricdetectionmethodforthepesticidedeltamethrin AT jiafuwang screeningofaptamersandthedevelopmentofacolorimetricdetectionmethodforthepesticidedeltamethrin AT shengli screeningofaptamersandthedevelopmentofacolorimetricdetectionmethodforthepesticidedeltamethrin |