Transcriptional profile of the rat cardiovascular system at single-cell resolution
Summary: We sought to characterize cellular composition across the cardiovascular system of the healthy Wistar rat, an important model in preclinical cardiovascular research. We performed single-nucleus RNA sequencing (snRNA-seq) in 78 samples in 10 distinct regions, including the four chambers of t...
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Elsevier
2025-01-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124724014426 |
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| author | Alessandro Arduini Stephen J. Fleming Ling Xiao Amelia W. Hall Amer-Denis Akkad Mark D. Chaffin Kayla J. Bendinelli Nathan R. Tucker Irinna Papangeli Helene Mantineo Patricio Flores-Bringas Mehrtash Babadi Christian M. Stegmann Guillermo García-Cardeña Mark E. Lindsay Carla Klattenhoff Patrick T. Ellinor |
| author_facet | Alessandro Arduini Stephen J. Fleming Ling Xiao Amelia W. Hall Amer-Denis Akkad Mark D. Chaffin Kayla J. Bendinelli Nathan R. Tucker Irinna Papangeli Helene Mantineo Patricio Flores-Bringas Mehrtash Babadi Christian M. Stegmann Guillermo García-Cardeña Mark E. Lindsay Carla Klattenhoff Patrick T. Ellinor |
| author_sort | Alessandro Arduini |
| collection | DOAJ |
| description | Summary: We sought to characterize cellular composition across the cardiovascular system of the healthy Wistar rat, an important model in preclinical cardiovascular research. We performed single-nucleus RNA sequencing (snRNA-seq) in 78 samples in 10 distinct regions, including the four chambers of the heart, ventricular septum, sinoatrial node, atrioventricular node, aorta, pulmonary artery, and pulmonary veins, which produced 505,835 nuclei. We identified 26 distinct cell types and additional subtypes, with different cellular composition across cardiac regions and tissue-specific transcription for each cell type. Several cell subtypes were region specific, including a subtype of vascular smooth muscle cells enriched in the large vasculature. We observed tissue-enriched cellular communication networks, including heightened Nppa-Npr1/2/3 signaling in the sinoatrial node. The existence of tissue-restricted cell types suggests regional regulation of cardiovascular physiology. Our detailed transcriptional characterization of each cell type offers the potential to identify novel therapeutic targets and improve preclinical models of cardiovascular disease. |
| format | Article |
| id | doaj-art-0767ac83a4d74870a6d56da8ae01844d |
| institution | DOAJ |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-0767ac83a4d74870a6d56da8ae01844d2025-08-20T02:40:24ZengElsevierCell Reports2211-12472025-01-0144111509110.1016/j.celrep.2024.115091Transcriptional profile of the rat cardiovascular system at single-cell resolutionAlessandro Arduini0Stephen J. Fleming1Ling Xiao2Amelia W. Hall3Amer-Denis Akkad4Mark D. Chaffin5Kayla J. Bendinelli6Nathan R. Tucker7Irinna Papangeli8Helene Mantineo9Patricio Flores-Bringas10Mehrtash Babadi11Christian M. Stegmann12Guillermo García-Cardeña13Mark E. Lindsay14Carla Klattenhoff15Patrick T. Ellinor16Precision Cardiology Laboratory, The Broad Institute, Cambridge, MA 02142, USAPrecision Cardiology Laboratory, The Broad Institute, Cambridge, MA 02142, USA; Data Sciences Platform, The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USAPrecision Cardiology Laboratory, The Broad Institute, Cambridge, MA 02142, USA; Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA 02114, USAGene Regulation Observatory, The Broad Institute, Cambridge, MA 02142, USAPrecision Cardiology Laboratory, Bayer US LLC, Cambridge, MA 02142, USAPrecision Cardiology Laboratory, The Broad Institute, Cambridge, MA 02142, USAPrecision Cardiology Laboratory, The Broad Institute, Cambridge, MA 02142, USASUNY Upstate Medical University, Syracuse, NY 13210, USAPrecision Cardiology Laboratory, Bayer US LLC, Cambridge, MA 02142, USAPrecision Cardiology Laboratory, The Broad Institute, Cambridge, MA 02142, USA; Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA 02114, USAPrecision Cardiology Laboratory, The Broad Institute, Cambridge, MA 02142, USAData Sciences Platform, The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USAPrecision Cardiology Laboratory, Bayer US LLC, Cambridge, MA 02142, USAPrecision Cardiology Laboratory, The Broad Institute, Cambridge, MA 02142, USA; Department of Pathology, Brigham and Women’s Hospital, Boston, MA 02215, USAPrecision Cardiology Laboratory, The Broad Institute, Cambridge, MA 02142, USA; Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA 02114, USAPrecision Cardiology Laboratory, Bayer US LLC, Cambridge, MA 02142, USAPrecision Cardiology Laboratory, The Broad Institute, Cambridge, MA 02142, USA; Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA 02114, USA; Cardiology Division, Massachusetts General Hospital, Boston, MA 02114, USA; Corresponding authorSummary: We sought to characterize cellular composition across the cardiovascular system of the healthy Wistar rat, an important model in preclinical cardiovascular research. We performed single-nucleus RNA sequencing (snRNA-seq) in 78 samples in 10 distinct regions, including the four chambers of the heart, ventricular septum, sinoatrial node, atrioventricular node, aorta, pulmonary artery, and pulmonary veins, which produced 505,835 nuclei. We identified 26 distinct cell types and additional subtypes, with different cellular composition across cardiac regions and tissue-specific transcription for each cell type. Several cell subtypes were region specific, including a subtype of vascular smooth muscle cells enriched in the large vasculature. We observed tissue-enriched cellular communication networks, including heightened Nppa-Npr1/2/3 signaling in the sinoatrial node. The existence of tissue-restricted cell types suggests regional regulation of cardiovascular physiology. Our detailed transcriptional characterization of each cell type offers the potential to identify novel therapeutic targets and improve preclinical models of cardiovascular disease.http://www.sciencedirect.com/science/article/pii/S2211124724014426CP: Developmental biology |
| spellingShingle | Alessandro Arduini Stephen J. Fleming Ling Xiao Amelia W. Hall Amer-Denis Akkad Mark D. Chaffin Kayla J. Bendinelli Nathan R. Tucker Irinna Papangeli Helene Mantineo Patricio Flores-Bringas Mehrtash Babadi Christian M. Stegmann Guillermo García-Cardeña Mark E. Lindsay Carla Klattenhoff Patrick T. Ellinor Transcriptional profile of the rat cardiovascular system at single-cell resolution Cell Reports CP: Developmental biology |
| title | Transcriptional profile of the rat cardiovascular system at single-cell resolution |
| title_full | Transcriptional profile of the rat cardiovascular system at single-cell resolution |
| title_fullStr | Transcriptional profile of the rat cardiovascular system at single-cell resolution |
| title_full_unstemmed | Transcriptional profile of the rat cardiovascular system at single-cell resolution |
| title_short | Transcriptional profile of the rat cardiovascular system at single-cell resolution |
| title_sort | transcriptional profile of the rat cardiovascular system at single cell resolution |
| topic | CP: Developmental biology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124724014426 |
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