ATP Synthase β-Chain Overexpression in SR-BI Knockout Mice Increases HDL Uptake and Reduces Plasma HDL Level
HDL cholesterol is known to be inversely correlated with cardiovascular disease due to its diverse antiatherogenic functions. SR-BI mediates the selective uptake of HDL-C. SR-BI knockout diminishes but does not completely block the transport of HDL; other receptors may be involved. Ectopic ATP synth...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2014/356432 |
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| author | Kexiu Song Yingchun Han Linhua Zhang Guoqing Liu Peng Yang Xiaoyun Cheng Le Bu Hui Sheng Shen Qu |
| author_facet | Kexiu Song Yingchun Han Linhua Zhang Guoqing Liu Peng Yang Xiaoyun Cheng Le Bu Hui Sheng Shen Qu |
| author_sort | Kexiu Song |
| collection | DOAJ |
| description | HDL cholesterol is known to be inversely correlated with cardiovascular disease due to its diverse antiatherogenic functions. SR-BI mediates the selective uptake of HDL-C. SR-BI knockout diminishes but does not completely block the transport of HDL; other receptors may be involved. Ectopic ATP synthase β-chain in hepatocytes has been previously characterized as an apoA-I receptor, triggering HDL internalization. This study was undertaken to identify the overexpression of ectopic ATP synthase β-chain on DIL-HDL uptake in primary hepatocytes in vitro and on plasma HDL levels in SR-BI knockout mice. Human ATP synthase β-chain cDNA was delivered to the mouse liver by adenovirus and GFP adenovirus as control. The adenovirus-mediated overexpression of β-chain was identified at both mRNA and protein levels on mice liver and validated by its increasing of DiL-HDL uptake in primary hepatocytes. In response to hepatic overexpression of β-chain, plasma HDL-C levels and cholesterol were reduced in SR-BI knockout mice, compared with the control. The present data suggest that ATP synthase β-chain can serve as the endocytic receptor of HDL, and its overexpression can reduce plasma HDL-C. |
| format | Article |
| id | doaj-art-0765140e45f6467495b8a2cbcae0dcff |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-0765140e45f6467495b8a2cbcae0dcff2025-08-20T03:55:28ZengWileyInternational Journal of Endocrinology1687-83371687-83452014-01-01201410.1155/2014/356432356432ATP Synthase β-Chain Overexpression in SR-BI Knockout Mice Increases HDL Uptake and Reduces Plasma HDL LevelKexiu Song0Yingchun Han1Linhua Zhang2Guoqing Liu3Peng Yang4Xiaoyun Cheng5Le Bu6Hui Sheng7Shen Qu8Department of Endocrinology, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaInstitute of Cardiovascular Science, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Peking University Health Science Center, Beijing 100191, ChinaDepartment of Endocrinology, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaInstitute of Cardiovascular Science, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Peking University Health Science Center, Beijing 100191, ChinaDepartment of Endocrinology, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaDepartment of Endocrinology, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaDepartment of Endocrinology, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaDepartment of Endocrinology, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaDepartment of Endocrinology, Shanghai Tenth People’s Hospital, Tongji University, 301 Middle Yanchang Road, Shanghai 200072, ChinaHDL cholesterol is known to be inversely correlated with cardiovascular disease due to its diverse antiatherogenic functions. SR-BI mediates the selective uptake of HDL-C. SR-BI knockout diminishes but does not completely block the transport of HDL; other receptors may be involved. Ectopic ATP synthase β-chain in hepatocytes has been previously characterized as an apoA-I receptor, triggering HDL internalization. This study was undertaken to identify the overexpression of ectopic ATP synthase β-chain on DIL-HDL uptake in primary hepatocytes in vitro and on plasma HDL levels in SR-BI knockout mice. Human ATP synthase β-chain cDNA was delivered to the mouse liver by adenovirus and GFP adenovirus as control. The adenovirus-mediated overexpression of β-chain was identified at both mRNA and protein levels on mice liver and validated by its increasing of DiL-HDL uptake in primary hepatocytes. In response to hepatic overexpression of β-chain, plasma HDL-C levels and cholesterol were reduced in SR-BI knockout mice, compared with the control. The present data suggest that ATP synthase β-chain can serve as the endocytic receptor of HDL, and its overexpression can reduce plasma HDL-C.http://dx.doi.org/10.1155/2014/356432 |
| spellingShingle | Kexiu Song Yingchun Han Linhua Zhang Guoqing Liu Peng Yang Xiaoyun Cheng Le Bu Hui Sheng Shen Qu ATP Synthase β-Chain Overexpression in SR-BI Knockout Mice Increases HDL Uptake and Reduces Plasma HDL Level International Journal of Endocrinology |
| title | ATP Synthase β-Chain Overexpression in SR-BI Knockout Mice Increases HDL Uptake and Reduces Plasma HDL Level |
| title_full | ATP Synthase β-Chain Overexpression in SR-BI Knockout Mice Increases HDL Uptake and Reduces Plasma HDL Level |
| title_fullStr | ATP Synthase β-Chain Overexpression in SR-BI Knockout Mice Increases HDL Uptake and Reduces Plasma HDL Level |
| title_full_unstemmed | ATP Synthase β-Chain Overexpression in SR-BI Knockout Mice Increases HDL Uptake and Reduces Plasma HDL Level |
| title_short | ATP Synthase β-Chain Overexpression in SR-BI Knockout Mice Increases HDL Uptake and Reduces Plasma HDL Level |
| title_sort | atp synthase β chain overexpression in sr bi knockout mice increases hdl uptake and reduces plasma hdl level |
| url | http://dx.doi.org/10.1155/2014/356432 |
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