Detection of Blastocystis species in immunocompromised patients (cancer, diabetes mellitus, and chronic renal diseases) by restriction fragment length polymorphism (RFLP)
Abstract Background Blastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans worldwide. Using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP), these subtypes are classified into fou...
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SpringerOpen
2025-04-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
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| Online Access: | https://doi.org/10.1186/s43088-025-00631-z |
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| author | Manal Z. M. Abdellatif Ekhlas H. Abdel-Hafeez Usama S. Belal Noha H. Abdelgelil Heba Marey Manar M. S. A. Nasr Mohamed Mahmoud Abdel-Fatah |
| author_facet | Manal Z. M. Abdellatif Ekhlas H. Abdel-Hafeez Usama S. Belal Noha H. Abdelgelil Heba Marey Manar M. S. A. Nasr Mohamed Mahmoud Abdel-Fatah |
| author_sort | Manal Z. M. Abdellatif |
| collection | DOAJ |
| description | Abstract Background Blastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans worldwide. Using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP), these subtypes are classified into four groups (A to D). This study aimed to detect Blastocystis spp. groups in immunocompromised patients and evaluate their correlation with clinical presentations. Materials and methods A cross-sectional study was conducted on 150 immunocompromised patients and 50 immunocompetent individuals. All subjects were evaluated for socio-demographic data, clinical features, and parasitic infections. RFLP analysis of the SSU rRNA gene was performed for Blastocystis spp. grouping. Results Among 150 immunocompromised patients, Blastocystis spp. was detected in 83.3% by direct wet mount and 94.7% by PCR, showing significant difference (P = 0.003) compared to immunocompetent individuals (40% and 50%, respectively). Of the PCR-positive immunocompromised patients, 86.7% were symptomatic, with diarrhea being the most common symptom (66%). In immunocompromised patients, group B of Blastocystis spp. subtypes (ST3, ST4, and ST8) dominated (86.7%). On the other hand, just 12% of immunocompetent people had symptoms, with group A subtypes (ST1 and ST2) accounting for 38% of the study cases. Conclusion Blastocystis spp. group B (ST3, ST4, and ST8) showed higher prevalence than group A (ST1 and ST2) in immunocompromised patients, with diarrhea being the most common clinical manifestation. |
| format | Article |
| id | doaj-art-074d7f4c014e4e098231393eba515657 |
| institution | OA Journals |
| issn | 2314-8543 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj-art-074d7f4c014e4e098231393eba5156572025-08-20T02:11:23ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432025-04-011411810.1186/s43088-025-00631-zDetection of Blastocystis species in immunocompromised patients (cancer, diabetes mellitus, and chronic renal diseases) by restriction fragment length polymorphism (RFLP)Manal Z. M. Abdellatif0Ekhlas H. Abdel-Hafeez1Usama S. Belal2Noha H. Abdelgelil3Heba Marey4Manar M. S. A. Nasr5Mohamed Mahmoud Abdel-Fatah6Minia UniversityMinia UniversityMinia UniversityMinia UniversityMinia UniversityMinia UniversityMinia UniversityAbstract Background Blastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans worldwide. Using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP), these subtypes are classified into four groups (A to D). This study aimed to detect Blastocystis spp. groups in immunocompromised patients and evaluate their correlation with clinical presentations. Materials and methods A cross-sectional study was conducted on 150 immunocompromised patients and 50 immunocompetent individuals. All subjects were evaluated for socio-demographic data, clinical features, and parasitic infections. RFLP analysis of the SSU rRNA gene was performed for Blastocystis spp. grouping. Results Among 150 immunocompromised patients, Blastocystis spp. was detected in 83.3% by direct wet mount and 94.7% by PCR, showing significant difference (P = 0.003) compared to immunocompetent individuals (40% and 50%, respectively). Of the PCR-positive immunocompromised patients, 86.7% were symptomatic, with diarrhea being the most common symptom (66%). In immunocompromised patients, group B of Blastocystis spp. subtypes (ST3, ST4, and ST8) dominated (86.7%). On the other hand, just 12% of immunocompetent people had symptoms, with group A subtypes (ST1 and ST2) accounting for 38% of the study cases. Conclusion Blastocystis spp. group B (ST3, ST4, and ST8) showed higher prevalence than group A (ST1 and ST2) in immunocompromised patients, with diarrhea being the most common clinical manifestation.https://doi.org/10.1186/s43088-025-00631-zBlastocystis spp.GroupingImmuno-compromisedPCR–RFLP |
| spellingShingle | Manal Z. M. Abdellatif Ekhlas H. Abdel-Hafeez Usama S. Belal Noha H. Abdelgelil Heba Marey Manar M. S. A. Nasr Mohamed Mahmoud Abdel-Fatah Detection of Blastocystis species in immunocompromised patients (cancer, diabetes mellitus, and chronic renal diseases) by restriction fragment length polymorphism (RFLP) Beni-Suef University Journal of Basic and Applied Sciences Blastocystis spp. Grouping Immuno-compromised PCR–RFLP |
| title | Detection of Blastocystis species in immunocompromised patients (cancer, diabetes mellitus, and chronic renal diseases) by restriction fragment length polymorphism (RFLP) |
| title_full | Detection of Blastocystis species in immunocompromised patients (cancer, diabetes mellitus, and chronic renal diseases) by restriction fragment length polymorphism (RFLP) |
| title_fullStr | Detection of Blastocystis species in immunocompromised patients (cancer, diabetes mellitus, and chronic renal diseases) by restriction fragment length polymorphism (RFLP) |
| title_full_unstemmed | Detection of Blastocystis species in immunocompromised patients (cancer, diabetes mellitus, and chronic renal diseases) by restriction fragment length polymorphism (RFLP) |
| title_short | Detection of Blastocystis species in immunocompromised patients (cancer, diabetes mellitus, and chronic renal diseases) by restriction fragment length polymorphism (RFLP) |
| title_sort | detection of blastocystis species in immunocompromised patients cancer diabetes mellitus and chronic renal diseases by restriction fragment length polymorphism rflp |
| topic | Blastocystis spp. Grouping Immuno-compromised PCR–RFLP |
| url | https://doi.org/10.1186/s43088-025-00631-z |
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