Influence of Fungal Colonization on Exacerbations in Patients with Cystic Fibrosis
The importance of fungal pathogens in cystic fibrosis (CF) patients and their diagnosis remains a challenge, so our aim was to analyze the influence of the detection of fungi in sputum by using conventional culture and molecular techniques, polymerase chain reaction (PCR), lateral flow devices (LFDs...
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MDPI AG
2024-12-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/10/12/875 |
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| author | Claudia Janeth Madrid-Carbajal Teresa Peláez-García de la Rasilla Marta Iscar-Urrutia Marta Solís-García Ramón Fernández-Álvarez Liliana Pérez-Martínez María Soledad Zapico-González Marta Garcia-Clemente |
| author_facet | Claudia Janeth Madrid-Carbajal Teresa Peláez-García de la Rasilla Marta Iscar-Urrutia Marta Solís-García Ramón Fernández-Álvarez Liliana Pérez-Martínez María Soledad Zapico-González Marta Garcia-Clemente |
| author_sort | Claudia Janeth Madrid-Carbajal |
| collection | DOAJ |
| description | The importance of fungal pathogens in cystic fibrosis (CF) patients and their diagnosis remains a challenge, so our aim was to analyze the influence of the detection of fungi in sputum by using conventional culture and molecular techniques, polymerase chain reaction (PCR), lateral flow devices (LFDs), and galactomannan (GM) on exacerbations in patients with cystic fibrosis. A prospective study was conducted in patients via follow-up in the CF Unit of the Central University Hospital of Asturias from January 2021 to April 2022. Adult patients with at least one documented exacerbation were included. A complete fungal analysis of sputum samples was performed both in a period of clinical stability and in the exacerbation period. The microbiological study included conventional cultures for fungi, qPCR (polymerase chain reaction), LFDs (lateral flow devices), and galactomannan (GM) in sputum. We found that there were changes in their detection according to whether the patient is in a period of clinical stability or exacerbation; the positivity of the molecular tests and biomarkers in the period of exacerbation increased by 14%, 25%, and 21% for the analysis by qPCR, GM, and LFDs for <i>Aspergillus</i> and by 15% for the sputum culture for <i>Aspergillus</i>, which may mean that fungal isolates may play a role in the exacerbations of these patients. |
| format | Article |
| id | doaj-art-074008e46ca248b8aed2afd05a4e0b2a |
| institution | OA Journals |
| issn | 2309-608X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-074008e46ca248b8aed2afd05a4e0b2a2025-08-20T02:00:42ZengMDPI AGJournal of Fungi2309-608X2024-12-01101287510.3390/jof10120875Influence of Fungal Colonization on Exacerbations in Patients with Cystic FibrosisClaudia Janeth Madrid-Carbajal0Teresa Peláez-García de la Rasilla1Marta Iscar-Urrutia2Marta Solís-García3Ramón Fernández-Álvarez4Liliana Pérez-Martínez5María Soledad Zapico-González6Marta Garcia-Clemente7Pneumology Department, Central Universitary Hospital of Asturias (HUCA), 33011 Oviedo, SpainMicrobiology Department, Central Universitary Hospital of Asturias (HUCA), 33011 Oviedo, SpainPneumology Department, Central Universitary Hospital of Asturias (HUCA), 33011 Oviedo, SpainPneumology Department, Central Universitary Hospital of Asturias (HUCA), 33011 Oviedo, SpainPneumology Department, Central Universitary Hospital of Asturias (HUCA), 33011 Oviedo, SpainPneumology Department, Central Universitary Hospital of Asturias (HUCA), 33011 Oviedo, SpainMicrobiology Department, Central Universitary Hospital of Asturias (HUCA), 33011 Oviedo, SpainPneumology Department, Central Universitary Hospital of Asturias (HUCA), 33011 Oviedo, SpainThe importance of fungal pathogens in cystic fibrosis (CF) patients and their diagnosis remains a challenge, so our aim was to analyze the influence of the detection of fungi in sputum by using conventional culture and molecular techniques, polymerase chain reaction (PCR), lateral flow devices (LFDs), and galactomannan (GM) on exacerbations in patients with cystic fibrosis. A prospective study was conducted in patients via follow-up in the CF Unit of the Central University Hospital of Asturias from January 2021 to April 2022. Adult patients with at least one documented exacerbation were included. A complete fungal analysis of sputum samples was performed both in a period of clinical stability and in the exacerbation period. The microbiological study included conventional cultures for fungi, qPCR (polymerase chain reaction), LFDs (lateral flow devices), and galactomannan (GM) in sputum. We found that there were changes in their detection according to whether the patient is in a period of clinical stability or exacerbation; the positivity of the molecular tests and biomarkers in the period of exacerbation increased by 14%, 25%, and 21% for the analysis by qPCR, GM, and LFDs for <i>Aspergillus</i> and by 15% for the sputum culture for <i>Aspergillus</i>, which may mean that fungal isolates may play a role in the exacerbations of these patients.https://www.mdpi.com/2309-608X/10/12/875fibrosis cysticaspergillosisdiagnosticfungal screeningmolecular techniques |
| spellingShingle | Claudia Janeth Madrid-Carbajal Teresa Peláez-García de la Rasilla Marta Iscar-Urrutia Marta Solís-García Ramón Fernández-Álvarez Liliana Pérez-Martínez María Soledad Zapico-González Marta Garcia-Clemente Influence of Fungal Colonization on Exacerbations in Patients with Cystic Fibrosis Journal of Fungi fibrosis cystic aspergillosis diagnostic fungal screening molecular techniques |
| title | Influence of Fungal Colonization on Exacerbations in Patients with Cystic Fibrosis |
| title_full | Influence of Fungal Colonization on Exacerbations in Patients with Cystic Fibrosis |
| title_fullStr | Influence of Fungal Colonization on Exacerbations in Patients with Cystic Fibrosis |
| title_full_unstemmed | Influence of Fungal Colonization on Exacerbations in Patients with Cystic Fibrosis |
| title_short | Influence of Fungal Colonization on Exacerbations in Patients with Cystic Fibrosis |
| title_sort | influence of fungal colonization on exacerbations in patients with cystic fibrosis |
| topic | fibrosis cystic aspergillosis diagnostic fungal screening molecular techniques |
| url | https://www.mdpi.com/2309-608X/10/12/875 |
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