The Gαo Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons
Mastoparan-7 (Mas-7), an analogue of the peptide mastoparan, which is derived from wasp venom, is a direct activator of Pertussis toxin- (PTX-) sensitive G proteins. Mas-7 produces several biological effects in different cell types; however, little is known about how Mas-7 influences mature hippocam...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2016/4258171 |
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| author | Valerie T. Ramírez Eva Ramos-Fernández Nibaldo C. Inestrosa |
| author_facet | Valerie T. Ramírez Eva Ramos-Fernández Nibaldo C. Inestrosa |
| author_sort | Valerie T. Ramírez |
| collection | DOAJ |
| description | Mastoparan-7 (Mas-7), an analogue of the peptide mastoparan, which is derived from wasp venom, is a direct activator of Pertussis toxin- (PTX-) sensitive G proteins. Mas-7 produces several biological effects in different cell types; however, little is known about how Mas-7 influences mature hippocampal neurons. We examined the specific role of Mas-7 in the development of dendritic spines, the sites of excitatory synaptic contact that are crucial for synaptic plasticity. We report here that exposure of hippocampal neurons to a low dose of Mas-7 increases dendritic spine density and spine head width in a time-dependent manner. Additionally, Mas-7 enhances postsynaptic density protein-95 (PSD-95) clustering in neurites and activates Gαo signaling, increasing the intracellular Ca2+ concentration. To define the role of signaling intermediates, we measured the levels of phosphorylated protein kinase C (PKC), c-Jun N-terminal kinase (JNK), and calcium-calmodulin dependent protein kinase IIα (CaMKIIα) after Mas-7 treatment and determined that CaMKII activation is necessary for the Mas-7-dependent increase in dendritic spine density. Our results demonstrate a critical role for Gαo subunit signaling in the regulation of synapse formation. |
| format | Article |
| id | doaj-art-073fb345bf1b4f13ab57aeffdf71d3be |
| institution | OA Journals |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-073fb345bf1b4f13ab57aeffdf71d3be2025-08-20T02:02:48ZengWileyNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/42581714258171The Gαo Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal NeuronsValerie T. Ramírez0Eva Ramos-Fernández1Nibaldo C. Inestrosa2Centro de Envejecimiento y Regeneración (CARE), Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, 8331150 Santiago, ChileCentro de Envejecimiento y Regeneración (CARE), Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, 8331150 Santiago, ChileCentro de Envejecimiento y Regeneración (CARE), Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, 8331150 Santiago, ChileMastoparan-7 (Mas-7), an analogue of the peptide mastoparan, which is derived from wasp venom, is a direct activator of Pertussis toxin- (PTX-) sensitive G proteins. Mas-7 produces several biological effects in different cell types; however, little is known about how Mas-7 influences mature hippocampal neurons. We examined the specific role of Mas-7 in the development of dendritic spines, the sites of excitatory synaptic contact that are crucial for synaptic plasticity. We report here that exposure of hippocampal neurons to a low dose of Mas-7 increases dendritic spine density and spine head width in a time-dependent manner. Additionally, Mas-7 enhances postsynaptic density protein-95 (PSD-95) clustering in neurites and activates Gαo signaling, increasing the intracellular Ca2+ concentration. To define the role of signaling intermediates, we measured the levels of phosphorylated protein kinase C (PKC), c-Jun N-terminal kinase (JNK), and calcium-calmodulin dependent protein kinase IIα (CaMKIIα) after Mas-7 treatment and determined that CaMKII activation is necessary for the Mas-7-dependent increase in dendritic spine density. Our results demonstrate a critical role for Gαo subunit signaling in the regulation of synapse formation.http://dx.doi.org/10.1155/2016/4258171 |
| spellingShingle | Valerie T. Ramírez Eva Ramos-Fernández Nibaldo C. Inestrosa The Gαo Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons Neural Plasticity |
| title | The Gαo Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons |
| title_full | The Gαo Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons |
| title_fullStr | The Gαo Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons |
| title_full_unstemmed | The Gαo Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons |
| title_short | The Gαo Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons |
| title_sort | gαo activator mastoparan 7 promotes dendritic spine formation in hippocampal neurons |
| url | http://dx.doi.org/10.1155/2016/4258171 |
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