Characteristics of the monkeypox virus isolate obtained from the first patient in Russia and its sensitivity to 7-[N-(4-trifluoromethylbenzoyl)-hydrazinocarbonyl]-tricyclo-[3.2.2.0^2,4]non-8-en-6-carboxylic acid
Introduction. Since early May 2022, more than 90,000 cases of monkeypox virus infection have been reported in more than 70 countries around the World. This is the largest outbreak of monkeypox ever recorded outside of Africa. The aim of the study is to confirm the first case of monkeypox in Russi...
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Central Research Institute for Epidemiology
2024-12-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
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| Online Access: | https://microbiol.crie.ru/jour/article/viewFile/18661/1549 |
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| author | Alena S. Ovchinnikova Dmitrii A. Odnoshevsky Alexey S. Kabanov Sergey A. Bodnev Oleg V. Pyankov Oksana P. Os'kina Maria V. Sivay Andrey V. Bespalov Tatyana V. Tregubchak Larisa N. Shishkina Oleg S. Taranov Vladimir V. Zolin Artemiy A. Sergeev Alexander P. Agafonov |
| author_facet | Alena S. Ovchinnikova Dmitrii A. Odnoshevsky Alexey S. Kabanov Sergey A. Bodnev Oleg V. Pyankov Oksana P. Os'kina Maria V. Sivay Andrey V. Bespalov Tatyana V. Tregubchak Larisa N. Shishkina Oleg S. Taranov Vladimir V. Zolin Artemiy A. Sergeev Alexander P. Agafonov |
| author_sort | Alena S. Ovchinnikova |
| collection | DOAJ |
| description | Introduction. Since early May 2022, more than 90,000 cases of monkeypox virus infection have been reported in more than 70 countries around the World. This is the largest outbreak of monkeypox ever recorded outside of Africa.
The aim of the study is to confirm the first case of monkeypox in Russia, to isolate and sequence a new strain of monkeypox virus (MPXV), and to assess its sensitivity to the 7-[N-(4-trifluoromethylbenzoyl)-hydrazinocarbonyl]-tricyclo-[3.2.2.0^2,4]non-8-en-6-carboxylic acid (NIOCH-14) antipox drug.
Materials and methods. The biological materials obtained from the affected area of the skin (contents of vesicles), a nasopharyngeal smear, sputum and venous blood from a patient with suspected monkeypox were used. The disease was confirmed by PCR followed by determination of the nucleotide sequence of viral DNA by sequencing. Isolation of the new MPXV strain from clinical samples was carried out in Vero E6 cells. The antiviral effectiveness of NIOCH-14 against the new MPXV strain was assessed using an adapted spectrophotometric method.
Results. A diagnostic study of the biological samples of a patient who returned from a tourist trip to European countries with complaints of skin rashes all over the body revealed MPXV DNA. A new strain of MPXV was isolated from vesicles in Vero E6 cells, and the genomic sequence MPXV-pustule S45 was assembled using high-throughput parallel sequencing (NGS).
Discussion. The effectiveness of the finished dosage form of NIOCH-14 against the new strain of MPXV based on the results of determining the 50% virus inhibitory concentration (IC50) was 0.02 μg/mL, and the selectivity index (SI) was 15,000.
Conclusion. In this study, the pathogen of monkeypox was detected and identified using real-time PCR, NGS and electron microscopy, and the first imported case of this disease in Russia was confirmed. It has been proven that the drug NIOCH-14 exhibits high antiviral activity in vitro against the new MPXV strain. |
| format | Article |
| id | doaj-art-073f97d6d0c4486eadd2d32c7ba94616 |
| institution | Kabale University |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| publishDate | 2024-12-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj-art-073f97d6d0c4486eadd2d32c7ba946162025-02-06T21:11:31ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132024-12-01101674875710.36233/0372-9311-5892797Characteristics of the monkeypox virus isolate obtained from the first patient in Russia and its sensitivity to 7-[N-(4-trifluoromethylbenzoyl)-hydrazinocarbonyl]-tricyclo-[3.2.2.0^2,4]non-8-en-6-carboxylic acidAlena S. Ovchinnikova0https://orcid.org/0000-0002-1745-7643Dmitrii A. Odnoshevsky1https://orcid.org/0000-0003-0616-7488Alexey S. Kabanov2https://orcid.org/0000-0002-6287-0912Sergey A. Bodnev3https://orcid.org/0000-0003-0599-3817Oleg V. Pyankov4https://orcid.org/0000-0003-3340-8750Oksana P. Os'kina5https://orcid.org/0000-0002-9165-1524Maria V. Sivay6https://orcid.org/0000-0002-4193-0779Andrey V. Bespalov7https://orcid.org/0000-0002-0281-3414Tatyana V. Tregubchak8https://orcid.org/0000-0001-9608-2044Larisa N. Shishkina9https://orcid.org/0000-0002-8264-0217Oleg S. Taranov10https://orcid.org/0000-0002-6746-8092Vladimir V. Zolin11https://orcid.org/0000-0003-4120-1178Artemiy A. Sergeev12https://orcid.org/0000-0002-3591-1571Alexander P. Agafonov13https://orcid.org/0000-0003-2577-0434State Scientific Center of Virology and Biotechnology "Vector"State Scientific Center of Virology and Biotechnology "Vector"State Scientific Center of Virology and Biotechnology "Vector"State Scientific Center of Virology and Biotechnology "Vector"State Scientific Center of Virology and Biotechnology "Vector"State Scientific Center of Virology and Biotechnology "Vector"State Scientific Center of Virology and Biotechnology "Vector"State Scientific Center of Virology and Biotechnology "Vector"State Scientific Center of Virology and Biotechnology "Vector"State Scientific Center of Virology and Biotechnology "Vector"State Scientific Center of Virology and Biotechnology "Vector"State Scientific Center of Virology and Biotechnology "Vector"State Scientific Center of Virology and Biotechnology "Vector"State Scientific Center of Virology and Biotechnology "Vector"Introduction. Since early May 2022, more than 90,000 cases of monkeypox virus infection have been reported in more than 70 countries around the World. This is the largest outbreak of monkeypox ever recorded outside of Africa. The aim of the study is to confirm the first case of monkeypox in Russia, to isolate and sequence a new strain of monkeypox virus (MPXV), and to assess its sensitivity to the 7-[N-(4-trifluoromethylbenzoyl)-hydrazinocarbonyl]-tricyclo-[3.2.2.0^2,4]non-8-en-6-carboxylic acid (NIOCH-14) antipox drug. Materials and methods. The biological materials obtained from the affected area of the skin (contents of vesicles), a nasopharyngeal smear, sputum and venous blood from a patient with suspected monkeypox were used. The disease was confirmed by PCR followed by determination of the nucleotide sequence of viral DNA by sequencing. Isolation of the new MPXV strain from clinical samples was carried out in Vero E6 cells. The antiviral effectiveness of NIOCH-14 against the new MPXV strain was assessed using an adapted spectrophotometric method. Results. A diagnostic study of the biological samples of a patient who returned from a tourist trip to European countries with complaints of skin rashes all over the body revealed MPXV DNA. A new strain of MPXV was isolated from vesicles in Vero E6 cells, and the genomic sequence MPXV-pustule S45 was assembled using high-throughput parallel sequencing (NGS). Discussion. The effectiveness of the finished dosage form of NIOCH-14 against the new strain of MPXV based on the results of determining the 50% virus inhibitory concentration (IC50) was 0.02 μg/mL, and the selectivity index (SI) was 15,000. Conclusion. In this study, the pathogen of monkeypox was detected and identified using real-time PCR, NGS and electron microscopy, and the first imported case of this disease in Russia was confirmed. It has been proven that the drug NIOCH-14 exhibits high antiviral activity in vitro against the new MPXV strain.https://microbiol.crie.ru/jour/article/viewFile/18661/1549monkeypox virusgenomic sequencing of virusespcrcytopathic effectantiviral activitynioch-14 |
| spellingShingle | Alena S. Ovchinnikova Dmitrii A. Odnoshevsky Alexey S. Kabanov Sergey A. Bodnev Oleg V. Pyankov Oksana P. Os'kina Maria V. Sivay Andrey V. Bespalov Tatyana V. Tregubchak Larisa N. Shishkina Oleg S. Taranov Vladimir V. Zolin Artemiy A. Sergeev Alexander P. Agafonov Characteristics of the monkeypox virus isolate obtained from the first patient in Russia and its sensitivity to 7-[N-(4-trifluoromethylbenzoyl)-hydrazinocarbonyl]-tricyclo-[3.2.2.0^2,4]non-8-en-6-carboxylic acid Журнал микробиологии, эпидемиологии и иммунобиологии monkeypox virus genomic sequencing of viruses pcr cytopathic effect antiviral activity nioch-14 |
| title | Characteristics of the monkeypox virus isolate obtained from the first patient in Russia and its sensitivity to 7-[N-(4-trifluoromethylbenzoyl)-hydrazinocarbonyl]-tricyclo-[3.2.2.0^2,4]non-8-en-6-carboxylic acid |
| title_full | Characteristics of the monkeypox virus isolate obtained from the first patient in Russia and its sensitivity to 7-[N-(4-trifluoromethylbenzoyl)-hydrazinocarbonyl]-tricyclo-[3.2.2.0^2,4]non-8-en-6-carboxylic acid |
| title_fullStr | Characteristics of the monkeypox virus isolate obtained from the first patient in Russia and its sensitivity to 7-[N-(4-trifluoromethylbenzoyl)-hydrazinocarbonyl]-tricyclo-[3.2.2.0^2,4]non-8-en-6-carboxylic acid |
| title_full_unstemmed | Characteristics of the monkeypox virus isolate obtained from the first patient in Russia and its sensitivity to 7-[N-(4-trifluoromethylbenzoyl)-hydrazinocarbonyl]-tricyclo-[3.2.2.0^2,4]non-8-en-6-carboxylic acid |
| title_short | Characteristics of the monkeypox virus isolate obtained from the first patient in Russia and its sensitivity to 7-[N-(4-trifluoromethylbenzoyl)-hydrazinocarbonyl]-tricyclo-[3.2.2.0^2,4]non-8-en-6-carboxylic acid |
| title_sort | characteristics of the monkeypox virus isolate obtained from the first patient in russia and its sensitivity to 7 n 4 trifluoromethylbenzoyl hydrazinocarbonyl tricyclo 3 2 2 0 2 4 non 8 en 6 carboxylic acid |
| topic | monkeypox virus genomic sequencing of viruses pcr cytopathic effect antiviral activity nioch-14 |
| url | https://microbiol.crie.ru/jour/article/viewFile/18661/1549 |
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