Exploring miR-301a-3p and osteosarcoma: from expression differences to mechanism of action
Abstract Objectives The pathogenesis of osteosarcoma remains inadequately understood. This study aims to investigate the role and underlying mechanisms of miR-301a-3p in osteosarcoma progression, providing a scientific basis for the development of effective therapeutic strategies. Methods Osteosarco...
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Springer
2025-05-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-025-02345-1 |
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| author | Tianying Liu Mintao Ma |
| author_facet | Tianying Liu Mintao Ma |
| author_sort | Tianying Liu |
| collection | DOAJ |
| description | Abstract Objectives The pathogenesis of osteosarcoma remains inadequately understood. This study aims to investigate the role and underlying mechanisms of miR-301a-3p in osteosarcoma progression, providing a scientific basis for the development of effective therapeutic strategies. Methods Osteosarcoma tissues and cells were collected and miR-301a-3p expression was detected by qRT-PCR and assessed the correlation between miR-301a-3p expression and clinical disease and prognosis of osteosarcoma patients. The effects of miR-301a-3p overexpression on osteosarcoma cells were investigated by Transwell assay and CCK-8 assay. The targeting relationship between miR-301a-3p and MAFB was detected by dual luciferase reporter assay. Results miR-301a-3p was significantly downregulated in osteosarcoma. Significant differences in TNM staging and lung metastasis were observed between the high and low miR-301a-3p expression groups, with a higher mortality rate in the low expression group, indicating it as a risk factor for poor prognosis. Upregulation of miR-301a-3p inhibited the proliferation and metastasis of osteosarcoma cells. MAFB was identified as a target gene of miR-301a-3p, capable of reversing the inhibitory effects of miR-301a-3p on cancer cells. Conclusions Low expression of miR-301a-3p may serve as a warning sign for disease progression and poor prognosis in osteosarcoma patients. miR-301a-3p may inhibit malignant behaviors of osteosarcoma cells by targeting MAFB. Future studies could explore the integration of ultrasound-targeted microbubble destruction technology to enhance the targeted delivery of miR-301a-3p mimics or MAFB inhibitors, potentially overcoming limitations in drug penetration and bioavailability observed in conventional therapies. |
| format | Article |
| id | doaj-art-0732adba2ff342cd9419f912875267c7 |
| institution | Kabale University |
| issn | 2730-6011 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-0732adba2ff342cd9419f912875267c72025-08-20T03:53:46ZengSpringerDiscover Oncology2730-60112025-05-011611910.1007/s12672-025-02345-1Exploring miR-301a-3p and osteosarcoma: from expression differences to mechanism of actionTianying Liu0Mintao Ma1Department of Ultrasonography, Xidian Group HospitalDepartment of Ultrasound, The Affiliated Hospital of Northwest University/Xi’An No.3 HospitalAbstract Objectives The pathogenesis of osteosarcoma remains inadequately understood. This study aims to investigate the role and underlying mechanisms of miR-301a-3p in osteosarcoma progression, providing a scientific basis for the development of effective therapeutic strategies. Methods Osteosarcoma tissues and cells were collected and miR-301a-3p expression was detected by qRT-PCR and assessed the correlation between miR-301a-3p expression and clinical disease and prognosis of osteosarcoma patients. The effects of miR-301a-3p overexpression on osteosarcoma cells were investigated by Transwell assay and CCK-8 assay. The targeting relationship between miR-301a-3p and MAFB was detected by dual luciferase reporter assay. Results miR-301a-3p was significantly downregulated in osteosarcoma. Significant differences in TNM staging and lung metastasis were observed between the high and low miR-301a-3p expression groups, with a higher mortality rate in the low expression group, indicating it as a risk factor for poor prognosis. Upregulation of miR-301a-3p inhibited the proliferation and metastasis of osteosarcoma cells. MAFB was identified as a target gene of miR-301a-3p, capable of reversing the inhibitory effects of miR-301a-3p on cancer cells. Conclusions Low expression of miR-301a-3p may serve as a warning sign for disease progression and poor prognosis in osteosarcoma patients. miR-301a-3p may inhibit malignant behaviors of osteosarcoma cells by targeting MAFB. Future studies could explore the integration of ultrasound-targeted microbubble destruction technology to enhance the targeted delivery of miR-301a-3p mimics or MAFB inhibitors, potentially overcoming limitations in drug penetration and bioavailability observed in conventional therapies.https://doi.org/10.1007/s12672-025-02345-1OsteosarcomamiR-301a-3pPrognosisMAFBCellular processes |
| spellingShingle | Tianying Liu Mintao Ma Exploring miR-301a-3p and osteosarcoma: from expression differences to mechanism of action Discover Oncology Osteosarcoma miR-301a-3p Prognosis MAFB Cellular processes |
| title | Exploring miR-301a-3p and osteosarcoma: from expression differences to mechanism of action |
| title_full | Exploring miR-301a-3p and osteosarcoma: from expression differences to mechanism of action |
| title_fullStr | Exploring miR-301a-3p and osteosarcoma: from expression differences to mechanism of action |
| title_full_unstemmed | Exploring miR-301a-3p and osteosarcoma: from expression differences to mechanism of action |
| title_short | Exploring miR-301a-3p and osteosarcoma: from expression differences to mechanism of action |
| title_sort | exploring mir 301a 3p and osteosarcoma from expression differences to mechanism of action |
| topic | Osteosarcoma miR-301a-3p Prognosis MAFB Cellular processes |
| url | https://doi.org/10.1007/s12672-025-02345-1 |
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