Association of fracture configuration and callus formation with a concentration of proinflammatory cytokines in children with long bone fractures
Background/Aim. The inflammatory response is of utmost importance in bone healing, but the precise role of cells and cytokines remains unclear. In our study, we examined the as-sociation between interleukin-1β (IL-1β), tumor necrosis fac-tors alpha (TNF-α), monocyte chemoattractant protein-1 (MCP-1)...
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| Format: | Article |
| Language: | English |
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2021-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900078P.pdf |
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| author | Paunović Zoran Milutinović Sanja Stanković Nikola Abazović Džihan Stanojević Ivan Rakić Mia Đukić Mirjana Šupić Gordana Vojvodić Danilo Marić Dušan Khan Wasim S. Starčević Srđan |
| author_facet | Paunović Zoran Milutinović Sanja Stanković Nikola Abazović Džihan Stanojević Ivan Rakić Mia Đukić Mirjana Šupić Gordana Vojvodić Danilo Marić Dušan Khan Wasim S. Starčević Srđan |
| author_sort | Paunović Zoran |
| collection | DOAJ |
| description | Background/Aim. The inflammatory response is of utmost importance in bone healing, but the precise role of cells and cytokines remains unclear. In our study, we examined the as-sociation between interleukin-1β (IL-1β), tumor necrosis fac-tors alpha (TNF-α), monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) concentrations, fracture configuration, and callus formation. Methods. Serum cyto-kine concentrations were determined in 78 non-obese chil-dren with long bone fractures (group 1), 10 children with fin-ger fractures (group 2), and 10 healthy controls (group 3). Blood samples were taken immediately after fracture upon hospital admission for groups 1 and 2. Differences in cyto-kine concentrations were analyzed among groups and catego-rized according to fracture configuration and callus for-mation. Results. IL-1β and TNF-α levels were lower in pa-tients that went on to produce incomplete callus compared with patients that formed complete callus. Surprisingly, the average IL-1β concentration was highest in the healthy con-trol group. The only significant correlation between IL-1β and TNF-α was in the group with moderate callus formation. MCP-1 level was slightly increased in all patient groups com-pared to controls, with no mutual difference. An average IL-8 level showed a clear decrease tendency from the group with incompletely formed callus toward the group with completely formed callus compared to controls, without significant dif-ference. Children with epiphysiolysis had the lowest concen-trations of cytokines compared with all other fracture types including transverse, oblique, and spiral. There were signifi-cantly lower concentrations of IL-1β and MCP-1 in patients with less fragment displacement compared with patients with greater fragment displacement. Conclusion. The systemic in-flammatory response is important in physiological bone heal-ing. High early production of IL-1β, TNF-α, and MCP-1 is associated with greater callus formation and better healing outcome, while increased IL-8 level is associated with poor callus formation and worse healing outcome. Our results in-dicate that epiphysiolysis and larger fragment displacement are associated with delayed fracture healing. |
| format | Article |
| id | doaj-art-0727ffd5214b4bf698f1bfd64e12f196 |
| institution | OA Journals |
| issn | 0042-8450 2406-0720 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-0727ffd5214b4bf698f1bfd64e12f1962025-08-20T01:59:10ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502406-07202021-01-0178439740210.2298/VSP190526078P0042-84501900078PAssociation of fracture configuration and callus formation with a concentration of proinflammatory cytokines in children with long bone fracturesPaunović Zoran0Milutinović Sanja1https://orcid.org/0000-0003-3225-3793Stanković Nikola2Abazović Džihan3Stanojević Ivan4Rakić Mia5https://orcid.org/0000-0001-7093-7956Đukić Mirjana6Šupić Gordana7https://orcid.org/0000-0003-0350-4733Vojvodić Danilo8https://orcid.org/0000-0001-9308-3076Marić Dušan9Khan Wasim S.10Starčević Srđan11Institute for Health Protection of Mother and Child of Serbia “Dr. Vukan Čupić“, Belgrade, SerbiaUniversity of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, SerbiaInstitute for Health Protection of Mother and Child of Serbia “Dr. Vukan Čupić“, Belgrade, SerbiaEmergency Medical Center of Montenegro, Podgorica, MontenegroUniversity of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia + Military Medical Academy, Institute for Medical Research, Belgrade, SerbiaUniversity of Nantes, Faculty of Dental Surgery, Nantes, France + University of Belgrade, Institute for Biological Research “Siniša Stanković“, Belgrade, Serbia University of Belgrade, Faculty of Pharmacy, Belgrade, SerbiaUniversity of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia + Military Medical Academy, Institute for Medical Research, Belgrade, SerbiaUniversity of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia + Military Medical Academy, Institute for Medical Research, Belgrade, SerbiaUniversity of Novi Sad, Faculty of Medicine, Novi Sad, SerbianemaUniversity of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia + Military Medical Academy,Clinic for Orthopedics and Traumatology, Belgrade, SerbiaBackground/Aim. The inflammatory response is of utmost importance in bone healing, but the precise role of cells and cytokines remains unclear. In our study, we examined the as-sociation between interleukin-1β (IL-1β), tumor necrosis fac-tors alpha (TNF-α), monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) concentrations, fracture configuration, and callus formation. Methods. Serum cyto-kine concentrations were determined in 78 non-obese chil-dren with long bone fractures (group 1), 10 children with fin-ger fractures (group 2), and 10 healthy controls (group 3). Blood samples were taken immediately after fracture upon hospital admission for groups 1 and 2. Differences in cyto-kine concentrations were analyzed among groups and catego-rized according to fracture configuration and callus for-mation. Results. IL-1β and TNF-α levels were lower in pa-tients that went on to produce incomplete callus compared with patients that formed complete callus. Surprisingly, the average IL-1β concentration was highest in the healthy con-trol group. The only significant correlation between IL-1β and TNF-α was in the group with moderate callus formation. MCP-1 level was slightly increased in all patient groups com-pared to controls, with no mutual difference. An average IL-8 level showed a clear decrease tendency from the group with incompletely formed callus toward the group with completely formed callus compared to controls, without significant dif-ference. Children with epiphysiolysis had the lowest concen-trations of cytokines compared with all other fracture types including transverse, oblique, and spiral. There were signifi-cantly lower concentrations of IL-1β and MCP-1 in patients with less fragment displacement compared with patients with greater fragment displacement. Conclusion. The systemic in-flammatory response is important in physiological bone heal-ing. High early production of IL-1β, TNF-α, and MCP-1 is associated with greater callus formation and better healing outcome, while increased IL-8 level is associated with poor callus formation and worse healing outcome. Our results in-dicate that epiphysiolysis and larger fragment displacement are associated with delayed fracture healing.http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900078P.pdfprognosisfractures, bonefracture healingchildadolescentbony callusinterleukin-1betatumor necrosis factor-alphamonocyte chemoattractant protein-1interleukin-8prognosis |
| spellingShingle | Paunović Zoran Milutinović Sanja Stanković Nikola Abazović Džihan Stanojević Ivan Rakić Mia Đukić Mirjana Šupić Gordana Vojvodić Danilo Marić Dušan Khan Wasim S. Starčević Srđan Association of fracture configuration and callus formation with a concentration of proinflammatory cytokines in children with long bone fractures Vojnosanitetski Pregled prognosis fractures, bone fracture healing child adolescent bony callus interleukin-1beta tumor necrosis factor-alpha monocyte chemoattractant protein-1 interleukin-8 prognosis |
| title | Association of fracture configuration and callus formation with a concentration of proinflammatory cytokines in children with long bone fractures |
| title_full | Association of fracture configuration and callus formation with a concentration of proinflammatory cytokines in children with long bone fractures |
| title_fullStr | Association of fracture configuration and callus formation with a concentration of proinflammatory cytokines in children with long bone fractures |
| title_full_unstemmed | Association of fracture configuration and callus formation with a concentration of proinflammatory cytokines in children with long bone fractures |
| title_short | Association of fracture configuration and callus formation with a concentration of proinflammatory cytokines in children with long bone fractures |
| title_sort | association of fracture configuration and callus formation with a concentration of proinflammatory cytokines in children with long bone fractures |
| topic | prognosis fractures, bone fracture healing child adolescent bony callus interleukin-1beta tumor necrosis factor-alpha monocyte chemoattractant protein-1 interleukin-8 prognosis |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900078P.pdf |
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