Isolation and characterization of hexavalent chromium-tolerant endophytic bacteria inhabiting Solanum virginicum L. roots: A study on potential for chromium bioremediation and plant growth promotion
Present study was performed with the aim to isolate Heavy metal Tolerant- PGPB (HMT-PGPB) from metal-contaminated site and use them for Cr bioremediation. Six different bacterial strains were obtained from the endosphere of Solanum virginicum L. roots and cultured using nutrient agar media amended w...
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Elsevier
2024-11-01
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| Series: | Journal of Hazardous Materials Letters |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666911024000133 |
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| author | Akanksha Gupta Surendra Kumar Gond Virendra Kumar Mishra |
| author_facet | Akanksha Gupta Surendra Kumar Gond Virendra Kumar Mishra |
| author_sort | Akanksha Gupta |
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| description | Present study was performed with the aim to isolate Heavy metal Tolerant- PGPB (HMT-PGPB) from metal-contaminated site and use them for Cr bioremediation. Six different bacterial strains were obtained from the endosphere of Solanum virginicum L. roots and cultured using nutrient agar media amended with 20 mg/L of Cr(VI). The ability of these Cr(VI) tolerant bacterial isolates were assessed for PGP traits like producing siderophores, indole-3-acetic acid (IAA), and phosphate solubilization. The findings indicated that all of the isolates could produce exopolymeric substances and IAA, five of them could produce siderophores, and three could solubilize phosphate. Furthermore, the minimum inhibitory concentrations of these strains werealso determined. These strains were identified as Bacillus licheniformis SxR1, B. tequilensis SxR2, B. subtilis SxR3, B. velezensis SxR4, B. amyloliquefaciens SxR6, and B. stercoris SxR8. To validate the findings, it is crucial to comprehend how Cr(VI) affects Bacillus sp. SxR1 cells to determine the course of uptake and bacterial cell alteration, which was assessed via Fourier Transform-Infrared Spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM). |
| format | Article |
| id | doaj-art-0714c6c21f084e23ae6f2d50cc7a0313 |
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| issn | 2666-9110 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
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| series | Journal of Hazardous Materials Letters |
| spelling | doaj-art-0714c6c21f084e23ae6f2d50cc7a03132025-08-20T02:32:41ZengElsevierJournal of Hazardous Materials Letters2666-91102024-11-01510011410.1016/j.hazl.2024.100114Isolation and characterization of hexavalent chromium-tolerant endophytic bacteria inhabiting Solanum virginicum L. roots: A study on potential for chromium bioremediation and plant growth promotionAkanksha Gupta0Surendra Kumar Gond1Virendra Kumar Mishra2Institute of Environment and Sustainable Development, Banaras Hindu University, Varanasi 221005, IndiaDepartment of Botany, Mahila Maha Vidhyalaya, Banaras Hindu University, Varanasi 221005, India; Correspondence to: Institute of Environment & Sustainable Development, Banaras Hindu University, 221005, India.Institute of Environment and Sustainable Development, Banaras Hindu University, Varanasi 221005, India; Correspondence to: Department of Botany, Mahila Maha Vidhyalaya, Banaras Hindu University, 221005, India.Present study was performed with the aim to isolate Heavy metal Tolerant- PGPB (HMT-PGPB) from metal-contaminated site and use them for Cr bioremediation. Six different bacterial strains were obtained from the endosphere of Solanum virginicum L. roots and cultured using nutrient agar media amended with 20 mg/L of Cr(VI). The ability of these Cr(VI) tolerant bacterial isolates were assessed for PGP traits like producing siderophores, indole-3-acetic acid (IAA), and phosphate solubilization. The findings indicated that all of the isolates could produce exopolymeric substances and IAA, five of them could produce siderophores, and three could solubilize phosphate. Furthermore, the minimum inhibitory concentrations of these strains werealso determined. These strains were identified as Bacillus licheniformis SxR1, B. tequilensis SxR2, B. subtilis SxR3, B. velezensis SxR4, B. amyloliquefaciens SxR6, and B. stercoris SxR8. To validate the findings, it is crucial to comprehend how Cr(VI) affects Bacillus sp. SxR1 cells to determine the course of uptake and bacterial cell alteration, which was assessed via Fourier Transform-Infrared Spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM).http://www.sciencedirect.com/science/article/pii/S2666911024000133EndophyteCr(VI)PGPBMetal resistanceBioremediation |
| spellingShingle | Akanksha Gupta Surendra Kumar Gond Virendra Kumar Mishra Isolation and characterization of hexavalent chromium-tolerant endophytic bacteria inhabiting Solanum virginicum L. roots: A study on potential for chromium bioremediation and plant growth promotion Journal of Hazardous Materials Letters Endophyte Cr(VI) PGPB Metal resistance Bioremediation |
| title | Isolation and characterization of hexavalent chromium-tolerant endophytic bacteria inhabiting Solanum virginicum L. roots: A study on potential for chromium bioremediation and plant growth promotion |
| title_full | Isolation and characterization of hexavalent chromium-tolerant endophytic bacteria inhabiting Solanum virginicum L. roots: A study on potential for chromium bioremediation and plant growth promotion |
| title_fullStr | Isolation and characterization of hexavalent chromium-tolerant endophytic bacteria inhabiting Solanum virginicum L. roots: A study on potential for chromium bioremediation and plant growth promotion |
| title_full_unstemmed | Isolation and characterization of hexavalent chromium-tolerant endophytic bacteria inhabiting Solanum virginicum L. roots: A study on potential for chromium bioremediation and plant growth promotion |
| title_short | Isolation and characterization of hexavalent chromium-tolerant endophytic bacteria inhabiting Solanum virginicum L. roots: A study on potential for chromium bioremediation and plant growth promotion |
| title_sort | isolation and characterization of hexavalent chromium tolerant endophytic bacteria inhabiting solanum virginicum l roots a study on potential for chromium bioremediation and plant growth promotion |
| topic | Endophyte Cr(VI) PGPB Metal resistance Bioremediation |
| url | http://www.sciencedirect.com/science/article/pii/S2666911024000133 |
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