Reprogramming of Mouse Calvarial Osteoblasts into Induced Pluripotent Stem Cells
Previous studies have demonstrated the ability of reprogramming endochondral bone into induced pluripotent stem (iPS) cells, but whether similar phenomenon occurs in intramembranous bone remains to be determined. Here we adopted fluorescence-activated cell sorting-based strategy to isolate homogenou...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/5280793 |
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| _version_ | 1832556587505418240 |
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| author | Yinxiang Wang Jessica Aijia Liu Keith K. H. Leung Mai Har Sham Danny Chan Kathryn S. E. Cheah Martin Cheung |
| author_facet | Yinxiang Wang Jessica Aijia Liu Keith K. H. Leung Mai Har Sham Danny Chan Kathryn S. E. Cheah Martin Cheung |
| author_sort | Yinxiang Wang |
| collection | DOAJ |
| description | Previous studies have demonstrated the ability of reprogramming endochondral bone into induced pluripotent stem (iPS) cells, but whether similar phenomenon occurs in intramembranous bone remains to be determined. Here we adopted fluorescence-activated cell sorting-based strategy to isolate homogenous population of intramembranous calvarial osteoblasts from newborn transgenic mice carrying both Osx1-GFP::Cre and Oct4-EGFP transgenes. Following retroviral transduction of Yamanaka factors (Oct4, Sox2, Klf4, and c-Myc), enriched population of osteoblasts underwent silencing of Osx1-GFP::Cre expression at early stage of reprogramming followed by late activation of Oct4-EGFP expression in the resulting iPS cells. These osteoblast-derived iPS cells exhibited gene expression profiles akin to embryonic stem cells and were pluripotent as demonstrated by their ability to form teratomas comprising tissues from all germ layers and also contribute to tail tissue in chimera embryos. These data demonstrate that iPS cells can be generated from intramembranous osteoblasts. |
| format | Article |
| id | doaj-art-0710d492b9114c74aeb813b278b0addc |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-0710d492b9114c74aeb813b278b0addc2025-02-03T05:44:48ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/52807935280793Reprogramming of Mouse Calvarial Osteoblasts into Induced Pluripotent Stem CellsYinxiang Wang0Jessica Aijia Liu1Keith K. H. Leung2Mai Har Sham3Danny Chan4Kathryn S. E. Cheah5Martin Cheung6Department of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong KongDepartment of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong KongDepartment of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong KongDepartment of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong KongDepartment of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong KongDepartment of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong KongDepartment of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong KongPrevious studies have demonstrated the ability of reprogramming endochondral bone into induced pluripotent stem (iPS) cells, but whether similar phenomenon occurs in intramembranous bone remains to be determined. Here we adopted fluorescence-activated cell sorting-based strategy to isolate homogenous population of intramembranous calvarial osteoblasts from newborn transgenic mice carrying both Osx1-GFP::Cre and Oct4-EGFP transgenes. Following retroviral transduction of Yamanaka factors (Oct4, Sox2, Klf4, and c-Myc), enriched population of osteoblasts underwent silencing of Osx1-GFP::Cre expression at early stage of reprogramming followed by late activation of Oct4-EGFP expression in the resulting iPS cells. These osteoblast-derived iPS cells exhibited gene expression profiles akin to embryonic stem cells and were pluripotent as demonstrated by their ability to form teratomas comprising tissues from all germ layers and also contribute to tail tissue in chimera embryos. These data demonstrate that iPS cells can be generated from intramembranous osteoblasts.http://dx.doi.org/10.1155/2018/5280793 |
| spellingShingle | Yinxiang Wang Jessica Aijia Liu Keith K. H. Leung Mai Har Sham Danny Chan Kathryn S. E. Cheah Martin Cheung Reprogramming of Mouse Calvarial Osteoblasts into Induced Pluripotent Stem Cells Stem Cells International |
| title | Reprogramming of Mouse Calvarial Osteoblasts into Induced Pluripotent Stem Cells |
| title_full | Reprogramming of Mouse Calvarial Osteoblasts into Induced Pluripotent Stem Cells |
| title_fullStr | Reprogramming of Mouse Calvarial Osteoblasts into Induced Pluripotent Stem Cells |
| title_full_unstemmed | Reprogramming of Mouse Calvarial Osteoblasts into Induced Pluripotent Stem Cells |
| title_short | Reprogramming of Mouse Calvarial Osteoblasts into Induced Pluripotent Stem Cells |
| title_sort | reprogramming of mouse calvarial osteoblasts into induced pluripotent stem cells |
| url | http://dx.doi.org/10.1155/2018/5280793 |
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