Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60
Protective action of melatonin on human peripheral blood mononuclear cells exposed to gamma radiation Co60. Objective: toevaluate the protective effect of melatonin on peripheral blood mononuclear cells (PBMCs) exposed in vitro to ionizing radiation. Materialsand methods: PBMCs drawn from healthy vo...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pontificia Universidad Javeriana
2009-04-01
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| Series: | Universitas Scientiarum |
| Subjects: | |
| Online Access: | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1398/860 |
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| Summary: | Protective action of melatonin on human peripheral blood mononuclear cells exposed to gamma radiation Co60. Objective: toevaluate the protective effect of melatonin on peripheral blood mononuclear cells (PBMCs) exposed in vitro to ionizing radiation. Materialsand methods: PBMCs drawn from healthy volunteers were incubated with 0, 1x10-5, 1x10-6 y 1x10-7 M melatonin for 10 minutes beforebeing exposed to gamma radiation (300 cGy; Co60 source). Afterwards, DNA damage was evaluated with the comet assay. Results:PBMCs pretreated with melatonin showed comet tails shorter than those without the hormone treatment, as well as a lower percentage ofcells with severe DNA damage. Conclusion: melatonin doses of 1x10-5, 1x10-6 and 1x10-7 M provide in vitro protection to PBMCs fromDNA damage (single strand breaks and alkali-labile sites) induced by gamma radiation (300 cGy; Co60 source). |
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| ISSN: | 0122-7483 2027-1352 |