Optimization of Extraction Process of Total Flavonoids from Loquat Leaves and Its Protective Effect on H9C2 Cells in Hypoxia Injury
To study the reflux extraction process of total flavonoids from loquat leaves and to investigate its protective effect on H9C2 cells in hypoxia injury. An orthogonal test was utilized to optimize the reflux extraction process parameters, Na2S2O4 was used to induce myocardial cell hypoxia injury, MTT...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-10-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
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| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_951_24 |
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| author | Zhongcheng Ke Xiaoling Cheng Manting Zhao Xu Cheng Ting Ye Ziyang Cheng |
| author_facet | Zhongcheng Ke Xiaoling Cheng Manting Zhao Xu Cheng Ting Ye Ziyang Cheng |
| author_sort | Zhongcheng Ke |
| collection | DOAJ |
| description | To study the reflux extraction process of total flavonoids from loquat leaves and to investigate its protective effect on H9C2 cells in hypoxia injury. An orthogonal test was utilized to optimize the reflux extraction process parameters, Na2S2O4 was used to induce myocardial cell hypoxia injury, MTT assay was used to evaluate cell survival rate, CK, LDH, SOD, MDA were used to evaluate the hypoxia injury of H9C2 cells. The optimized process condition for reflux extraction was 10 times the amount of 70% ethanol for 3 times, each time for 2 h. The extracted content of TFL was 37.12 mg/g under this condition, and the TFL was found to increase cell survival rate, decrease the levels of CK, LDH, MDA, and improve SOD levels. The favored reflux extraction process of loquat leaves is stable and feasible, and the TFL have a good protective effect on H9C2 cells in hypoxia injury. |
| format | Article |
| id | doaj-art-06f6c03e2e014266bf37da6aa79984a9 |
| institution | DOAJ |
| issn | 0976-4879 0975-7406 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj-art-06f6c03e2e014266bf37da6aa79984a92025-08-20T03:08:32ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062025-10-0116414915310.4103/jpbs.jpbs_951_24Optimization of Extraction Process of Total Flavonoids from Loquat Leaves and Its Protective Effect on H9C2 Cells in Hypoxia InjuryZhongcheng KeXiaoling ChengManting ZhaoXu ChengTing YeZiyang ChengTo study the reflux extraction process of total flavonoids from loquat leaves and to investigate its protective effect on H9C2 cells in hypoxia injury. An orthogonal test was utilized to optimize the reflux extraction process parameters, Na2S2O4 was used to induce myocardial cell hypoxia injury, MTT assay was used to evaluate cell survival rate, CK, LDH, SOD, MDA were used to evaluate the hypoxia injury of H9C2 cells. The optimized process condition for reflux extraction was 10 times the amount of 70% ethanol for 3 times, each time for 2 h. The extracted content of TFL was 37.12 mg/g under this condition, and the TFL was found to increase cell survival rate, decrease the levels of CK, LDH, MDA, and improve SOD levels. The favored reflux extraction process of loquat leaves is stable and feasible, and the TFL have a good protective effect on H9C2 cells in hypoxia injury.https://journals.lww.com/10.4103/jpbs.jpbs_951_24extractionhypoxia injuryloquat leafprotective effecttotal flavonoids |
| spellingShingle | Zhongcheng Ke Xiaoling Cheng Manting Zhao Xu Cheng Ting Ye Ziyang Cheng Optimization of Extraction Process of Total Flavonoids from Loquat Leaves and Its Protective Effect on H9C2 Cells in Hypoxia Injury Journal of Pharmacy and Bioallied Sciences extraction hypoxia injury loquat leaf protective effect total flavonoids |
| title | Optimization of Extraction Process of Total Flavonoids from Loquat Leaves and Its Protective Effect on H9C2 Cells in Hypoxia Injury |
| title_full | Optimization of Extraction Process of Total Flavonoids from Loquat Leaves and Its Protective Effect on H9C2 Cells in Hypoxia Injury |
| title_fullStr | Optimization of Extraction Process of Total Flavonoids from Loquat Leaves and Its Protective Effect on H9C2 Cells in Hypoxia Injury |
| title_full_unstemmed | Optimization of Extraction Process of Total Flavonoids from Loquat Leaves and Its Protective Effect on H9C2 Cells in Hypoxia Injury |
| title_short | Optimization of Extraction Process of Total Flavonoids from Loquat Leaves and Its Protective Effect on H9C2 Cells in Hypoxia Injury |
| title_sort | optimization of extraction process of total flavonoids from loquat leaves and its protective effect on h9c2 cells in hypoxia injury |
| topic | extraction hypoxia injury loquat leaf protective effect total flavonoids |
| url | https://journals.lww.com/10.4103/jpbs.jpbs_951_24 |
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