SEX DETERMINATION OF FETUS PRENATAL FROM MATERNAL PLASMA BY USING DUPLEX PCR TECHNIQUE IN GOAT
The present study included determinationof fetal sex prenatal by analyzing free fetal DNA cells in maternal plasma by using dupplex PCR . At the first amplifying the DNA which extracted from male and non-pregnant female goat plasma which used as control to confirm the accuracy of this technique....
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| Format: | Article |
| Language: | English |
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College of Veterinary Medicine, University of Basrah, Iraq
2014-06-01
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| Series: | Basrah Journal of Veterinary Research |
| Subjects: | |
| Online Access: | https://bjvr.uobasrah.edu.iq/article_88125_65d52bcaaab68337aeaf8849033a86f6.pdf |
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| author | NisreenYasirJasim Tahir A. Fahid Talib Ahmed Jaayid |
| author_facet | NisreenYasirJasim Tahir A. Fahid Talib Ahmed Jaayid |
| author_sort | NisreenYasirJasim |
| collection | DOAJ |
| description | The present study included determinationof fetal sex prenatal by analyzing free fetal DNA cells in maternal plasma by using dupplex PCR . At the first amplifying the DNA which extracted from male and non-pregnant female goat plasma which used as control to confirm the accuracy of this technique. After thatblood were collected from 5 pregnant females goat prepared the plasma and extract the DNA by using heat-based direct method for plasma and then investigate the sequences GAPDH and DYS14 through the use of dupplex polymerase chain reaction technique and the results were 2 (40%) males show a double bands one at 97 bp representing GAPDH and the second at 198 bp representing DYS14 and 3 (60%) females show a single band at 97 bp representing GAPDH. This technique of sexing is applied for the first time on animals especially in Iraq. |
| format | Article |
| id | doaj-art-06eee8fac1414744842cd4787ccc1929 |
| institution | OA Journals |
| issn | 1813-8497 2410-8456 |
| language | English |
| publishDate | 2014-06-01 |
| publisher | College of Veterinary Medicine, University of Basrah, Iraq |
| record_format | Article |
| series | Basrah Journal of Veterinary Research |
| spelling | doaj-art-06eee8fac1414744842cd4787ccc19292025-08-20T02:31:33ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562014-06-01131505910.33762/bvetr.2014.8812588125SEX DETERMINATION OF FETUS PRENATAL FROM MATERNAL PLASMA BY USING DUPLEX PCR TECHNIQUE IN GOATNisreenYasirJasimTahir A. FahidTalib Ahmed JaayidThe present study included determinationof fetal sex prenatal by analyzing free fetal DNA cells in maternal plasma by using dupplex PCR . At the first amplifying the DNA which extracted from male and non-pregnant female goat plasma which used as control to confirm the accuracy of this technique. After thatblood were collected from 5 pregnant females goat prepared the plasma and extract the DNA by using heat-based direct method for plasma and then investigate the sequences GAPDH and DYS14 through the use of dupplex polymerase chain reaction technique and the results were 2 (40%) males show a double bands one at 97 bp representing GAPDH and the second at 198 bp representing DYS14 and 3 (60%) females show a single band at 97 bp representing GAPDH. This technique of sexing is applied for the first time on animals especially in Iraq.https://bjvr.uobasrah.edu.iq/article_88125_65d52bcaaab68337aeaf8849033a86f6.pdffetal sexgoatplasmaduplex pcr |
| spellingShingle | NisreenYasirJasim Tahir A. Fahid Talib Ahmed Jaayid SEX DETERMINATION OF FETUS PRENATAL FROM MATERNAL PLASMA BY USING DUPLEX PCR TECHNIQUE IN GOAT Basrah Journal of Veterinary Research fetal sex goat plasma duplex pcr |
| title | SEX DETERMINATION OF FETUS PRENATAL FROM MATERNAL PLASMA BY USING DUPLEX PCR TECHNIQUE IN GOAT |
| title_full | SEX DETERMINATION OF FETUS PRENATAL FROM MATERNAL PLASMA BY USING DUPLEX PCR TECHNIQUE IN GOAT |
| title_fullStr | SEX DETERMINATION OF FETUS PRENATAL FROM MATERNAL PLASMA BY USING DUPLEX PCR TECHNIQUE IN GOAT |
| title_full_unstemmed | SEX DETERMINATION OF FETUS PRENATAL FROM MATERNAL PLASMA BY USING DUPLEX PCR TECHNIQUE IN GOAT |
| title_short | SEX DETERMINATION OF FETUS PRENATAL FROM MATERNAL PLASMA BY USING DUPLEX PCR TECHNIQUE IN GOAT |
| title_sort | sex determination of fetus prenatal from maternal plasma by using duplex pcr technique in goat |
| topic | fetal sex goat plasma duplex pcr |
| url | https://bjvr.uobasrah.edu.iq/article_88125_65d52bcaaab68337aeaf8849033a86f6.pdf |
| work_keys_str_mv | AT nisreenyasirjasim sexdeterminationoffetusprenatalfrommaternalplasmabyusingduplexpcrtechniqueingoat AT tahirafahid sexdeterminationoffetusprenatalfrommaternalplasmabyusingduplexpcrtechniqueingoat AT talibahmedjaayid sexdeterminationoffetusprenatalfrommaternalplasmabyusingduplexpcrtechniqueingoat |