SEX DETERMINATION OF FETUS PRENATAL FROM MATERNAL PLASMA BY USING DUPLEX PCR TECHNIQUE IN GOAT
The present study included determinationof fetal sex prenatal by analyzing free fetal DNA cells in maternal plasma by using dupplex PCR . At the first amplifying the DNA which extracted from male and non-pregnant female goat plasma which used as control to confirm the accuracy of this technique....
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
College of Veterinary Medicine, University of Basrah, Iraq
2014-06-01
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| Series: | Basrah Journal of Veterinary Research |
| Subjects: | |
| Online Access: | https://bjvr.uobasrah.edu.iq/article_88125_65d52bcaaab68337aeaf8849033a86f6.pdf |
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| Summary: | The present study included determinationof fetal sex prenatal by analyzing free fetal DNA cells in maternal plasma by using dupplex PCR . At the first amplifying the DNA which extracted from male and non-pregnant female goat plasma which used as control to confirm the accuracy of this technique. After thatblood were collected from 5 pregnant females goat prepared the plasma and extract the DNA by using heat-based direct method for plasma and then investigate the sequences GAPDH and DYS14 through the use of dupplex polymerase chain reaction technique and the results were 2 (40%) males show a double bands one at 97 bp representing GAPDH and the second at 198 bp representing DYS14 and 3 (60%) females show a single band at 97 bp representing GAPDH. This technique of sexing is applied for the first time on animals especially in Iraq. |
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| ISSN: | 1813-8497 2410-8456 |