Microbial Synthesis of <i>Neo-Allo</i>-Ocimene by Celery-Derived <i>Neo-Allo</i>-Ocimene Synthase
<i>Neo-allo</i>-ocimene is a monoterpene which could be applied in pesticides, fragrances, and sustainable polymers. In this study, we mined a terpene synthase, AgTPS40, from the transcriptome of celery leaf tissues. Through sequence and phylogenetic analysis, AgTPS40 was characterized a...
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MDPI AG
2025-03-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/11/3/153 |
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| author | Zheng Liu Ting Gao Shaoheng Bao Penggang Han Ge Yao Tianyu Song Longbao Zhu Chang Chen Hui Jiang |
| author_facet | Zheng Liu Ting Gao Shaoheng Bao Penggang Han Ge Yao Tianyu Song Longbao Zhu Chang Chen Hui Jiang |
| author_sort | Zheng Liu |
| collection | DOAJ |
| description | <i>Neo-allo</i>-ocimene is a monoterpene which could be applied in pesticides, fragrances, and sustainable polymers. In this study, we mined a terpene synthase, AgTPS40, from the transcriptome of celery leaf tissues. Through sequence and phylogenetic analysis, AgTPS40 was characterized as a monoterpene synthase. The <i>AgTPS40</i> gene was introduced into a heterologous mevalonate pathway hosted in <i>Escherichia coli</i> to enable terpene production. Gas chromatography–mass spectrometry analysis confirmed that AgTPS40 catalyzes the formation of <i>neo-allo</i>-ocimene, marking the first reported identification of a <i>neo-allo</i>-ocimene synthase. Subsequently, we optimized the fermentation conditions and achieved a yield of 933.35 mg/L in a 1 L shake flask, which represents the highest reported titer of <i>neo-allo</i>-ocimene to date. These results reveal the molecular basis of <i>neo-allo</i>-ocimene synthesis in celery and provide a sustainable way to obtain this compound. |
| format | Article |
| id | doaj-art-06e70df8ce984ff99bcabe6ee29c2613 |
| institution | DOAJ |
| issn | 2311-5637 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-06e70df8ce984ff99bcabe6ee29c26132025-08-20T02:42:29ZengMDPI AGFermentation2311-56372025-03-0111315310.3390/fermentation11030153Microbial Synthesis of <i>Neo-Allo</i>-Ocimene by Celery-Derived <i>Neo-Allo</i>-Ocimene SynthaseZheng Liu0Ting Gao1Shaoheng Bao2Penggang Han3Ge Yao4Tianyu Song5Longbao Zhu6Chang Chen7Hui Jiang8School of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, ChinaState Key Laboratory of NBC Protection for Civilian, Beijing 102205, ChinaState Key Laboratory of NBC Protection for Civilian, Beijing 102205, ChinaState Key Laboratory of NBC Protection for Civilian, Beijing 102205, ChinaState Key Laboratory of NBC Protection for Civilian, Beijing 102205, ChinaState Key Laboratory of NBC Protection for Civilian, Beijing 102205, ChinaSchool of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, ChinaState Key Laboratory of NBC Protection for Civilian, Beijing 102205, ChinaState Key Laboratory of NBC Protection for Civilian, Beijing 102205, China<i>Neo-allo</i>-ocimene is a monoterpene which could be applied in pesticides, fragrances, and sustainable polymers. In this study, we mined a terpene synthase, AgTPS40, from the transcriptome of celery leaf tissues. Through sequence and phylogenetic analysis, AgTPS40 was characterized as a monoterpene synthase. The <i>AgTPS40</i> gene was introduced into a heterologous mevalonate pathway hosted in <i>Escherichia coli</i> to enable terpene production. Gas chromatography–mass spectrometry analysis confirmed that AgTPS40 catalyzes the formation of <i>neo-allo</i>-ocimene, marking the first reported identification of a <i>neo-allo</i>-ocimene synthase. Subsequently, we optimized the fermentation conditions and achieved a yield of 933.35 mg/L in a 1 L shake flask, which represents the highest reported titer of <i>neo-allo</i>-ocimene to date. These results reveal the molecular basis of <i>neo-allo</i>-ocimene synthesis in celery and provide a sustainable way to obtain this compound.https://www.mdpi.com/2311-5637/11/3/153celery<i>neo-allo</i>-ocimenemonoterpene synthasebiosynthesis |
| spellingShingle | Zheng Liu Ting Gao Shaoheng Bao Penggang Han Ge Yao Tianyu Song Longbao Zhu Chang Chen Hui Jiang Microbial Synthesis of <i>Neo-Allo</i>-Ocimene by Celery-Derived <i>Neo-Allo</i>-Ocimene Synthase Fermentation celery <i>neo-allo</i>-ocimene monoterpene synthase biosynthesis |
| title | Microbial Synthesis of <i>Neo-Allo</i>-Ocimene by Celery-Derived <i>Neo-Allo</i>-Ocimene Synthase |
| title_full | Microbial Synthesis of <i>Neo-Allo</i>-Ocimene by Celery-Derived <i>Neo-Allo</i>-Ocimene Synthase |
| title_fullStr | Microbial Synthesis of <i>Neo-Allo</i>-Ocimene by Celery-Derived <i>Neo-Allo</i>-Ocimene Synthase |
| title_full_unstemmed | Microbial Synthesis of <i>Neo-Allo</i>-Ocimene by Celery-Derived <i>Neo-Allo</i>-Ocimene Synthase |
| title_short | Microbial Synthesis of <i>Neo-Allo</i>-Ocimene by Celery-Derived <i>Neo-Allo</i>-Ocimene Synthase |
| title_sort | microbial synthesis of i neo allo i ocimene by celery derived i neo allo i ocimene synthase |
| topic | celery <i>neo-allo</i>-ocimene monoterpene synthase biosynthesis |
| url | https://www.mdpi.com/2311-5637/11/3/153 |
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