Microbial Synthesis of <i>Neo-Allo</i>-Ocimene by Celery-Derived <i>Neo-Allo</i>-Ocimene Synthase
<i>Neo-allo</i>-ocimene is a monoterpene which could be applied in pesticides, fragrances, and sustainable polymers. In this study, we mined a terpene synthase, AgTPS40, from the transcriptome of celery leaf tissues. Through sequence and phylogenetic analysis, AgTPS40 was characterized a...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
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| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/11/3/153 |
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| Summary: | <i>Neo-allo</i>-ocimene is a monoterpene which could be applied in pesticides, fragrances, and sustainable polymers. In this study, we mined a terpene synthase, AgTPS40, from the transcriptome of celery leaf tissues. Through sequence and phylogenetic analysis, AgTPS40 was characterized as a monoterpene synthase. The <i>AgTPS40</i> gene was introduced into a heterologous mevalonate pathway hosted in <i>Escherichia coli</i> to enable terpene production. Gas chromatography–mass spectrometry analysis confirmed that AgTPS40 catalyzes the formation of <i>neo-allo</i>-ocimene, marking the first reported identification of a <i>neo-allo</i>-ocimene synthase. Subsequently, we optimized the fermentation conditions and achieved a yield of 933.35 mg/L in a 1 L shake flask, which represents the highest reported titer of <i>neo-allo</i>-ocimene to date. These results reveal the molecular basis of <i>neo-allo</i>-ocimene synthesis in celery and provide a sustainable way to obtain this compound. |
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| ISSN: | 2311-5637 |