Urinary NGAL as an Early Marker of Renal Dysfunction in Dogs with Heartworm Disease and Pulmonary Hypertension

Heartworm disease, caused by <i>Dirofilaria immitis</i>, often leads to pulmonary hypertension (PH), a serious cardiovascular complication in infected dogs. PH may impair renal function through hemodynamic and inflammatory mechanisms, even when traditional biomarkers such as serum creati...

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Main Authors: Noelia Costa-Rodríguez, Daniel Julio Vera-Rodríguez, Soraya Falcón-Cordón, Beatriz Regina Morales, Rodrigo Morchón, José Alberto Montoya-Alonso, Elena Carretón
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/14/2003
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author Noelia Costa-Rodríguez
Daniel Julio Vera-Rodríguez
Soraya Falcón-Cordón
Beatriz Regina Morales
Rodrigo Morchón
José Alberto Montoya-Alonso
Elena Carretón
author_facet Noelia Costa-Rodríguez
Daniel Julio Vera-Rodríguez
Soraya Falcón-Cordón
Beatriz Regina Morales
Rodrigo Morchón
José Alberto Montoya-Alonso
Elena Carretón
author_sort Noelia Costa-Rodríguez
collection DOAJ
description Heartworm disease, caused by <i>Dirofilaria immitis</i>, often leads to pulmonary hypertension (PH), a serious cardiovascular complication in infected dogs. PH may impair renal function through hemodynamic and inflammatory mechanisms, even when traditional biomarkers such as serum creatinine and blood urea nitrogen (BUN) remain within normal ranges. This study aimed to assess urinary neutrophil gelatinase-associated lipocalin (uNGAL) levels in dogs naturally infected with <i>D. immitis</i>, with and without PH, to evaluate its potential as an early biomarker of renal dysfunction. Forty-two infected dogs were included and divided into two groups based on the presence (n = 14) or absence (n = 28) of PH, diagnosed via echocardiography. uNGAL concentrations were significantly higher in dogs with PH (mean 66.49 ± 6.67 ng/mL) compared to those without PH (mean 49.01 ± 14.48 ng/mL; <i>p</i> < 0.0001), despite normal creatinine and BUN values. No significant associations were found between uNGAL and sex, age, breed, or clinical signs. These findings suggest that uNGAL may serve as a sensitive biomarker of early renal impairment in dogs with heartworm disease and PH, even in the absence of overt azotemia, supporting its use in clinical evaluation and the monitoring of disease progression.
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spelling doaj-art-06e704fb8d434b2ca9322553a70c97692025-08-20T02:48:19ZengMDPI AGAnimals2076-26152025-07-011514200310.3390/ani15142003Urinary NGAL as an Early Marker of Renal Dysfunction in Dogs with Heartworm Disease and Pulmonary HypertensionNoelia Costa-Rodríguez0Daniel Julio Vera-Rodríguez1Soraya Falcón-Cordón2Beatriz Regina Morales3Rodrigo Morchón4José Alberto Montoya-Alonso5Elena Carretón6Internal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, SpainInternal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, SpainInternal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, SpainInternal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, SpainZoonotic Diseases and One Health Group, Laboratory of Parasitology, Faculty of Pharmacy, University of Salamanca, 37007 Salamanca, SpainInternal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, SpainInternal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, SpainHeartworm disease, caused by <i>Dirofilaria immitis</i>, often leads to pulmonary hypertension (PH), a serious cardiovascular complication in infected dogs. PH may impair renal function through hemodynamic and inflammatory mechanisms, even when traditional biomarkers such as serum creatinine and blood urea nitrogen (BUN) remain within normal ranges. This study aimed to assess urinary neutrophil gelatinase-associated lipocalin (uNGAL) levels in dogs naturally infected with <i>D. immitis</i>, with and without PH, to evaluate its potential as an early biomarker of renal dysfunction. Forty-two infected dogs were included and divided into two groups based on the presence (n = 14) or absence (n = 28) of PH, diagnosed via echocardiography. uNGAL concentrations were significantly higher in dogs with PH (mean 66.49 ± 6.67 ng/mL) compared to those without PH (mean 49.01 ± 14.48 ng/mL; <i>p</i> < 0.0001), despite normal creatinine and BUN values. No significant associations were found between uNGAL and sex, age, breed, or clinical signs. These findings suggest that uNGAL may serve as a sensitive biomarker of early renal impairment in dogs with heartworm disease and PH, even in the absence of overt azotemia, supporting its use in clinical evaluation and the monitoring of disease progression.https://www.mdpi.com/2076-2615/15/14/2003<i>Dirofilaria immitis</i>heartworm diseasecanineurinary NGALechocardiographypulmonary hypertension
spellingShingle Noelia Costa-Rodríguez
Daniel Julio Vera-Rodríguez
Soraya Falcón-Cordón
Beatriz Regina Morales
Rodrigo Morchón
José Alberto Montoya-Alonso
Elena Carretón
Urinary NGAL as an Early Marker of Renal Dysfunction in Dogs with Heartworm Disease and Pulmonary Hypertension
Animals
<i>Dirofilaria immitis</i>
heartworm disease
canine
urinary NGAL
echocardiography
pulmonary hypertension
title Urinary NGAL as an Early Marker of Renal Dysfunction in Dogs with Heartworm Disease and Pulmonary Hypertension
title_full Urinary NGAL as an Early Marker of Renal Dysfunction in Dogs with Heartworm Disease and Pulmonary Hypertension
title_fullStr Urinary NGAL as an Early Marker of Renal Dysfunction in Dogs with Heartworm Disease and Pulmonary Hypertension
title_full_unstemmed Urinary NGAL as an Early Marker of Renal Dysfunction in Dogs with Heartworm Disease and Pulmonary Hypertension
title_short Urinary NGAL as an Early Marker of Renal Dysfunction in Dogs with Heartworm Disease and Pulmonary Hypertension
title_sort urinary ngal as an early marker of renal dysfunction in dogs with heartworm disease and pulmonary hypertension
topic <i>Dirofilaria immitis</i>
heartworm disease
canine
urinary NGAL
echocardiography
pulmonary hypertension
url https://www.mdpi.com/2076-2615/15/14/2003
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